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Mol Cell Biol
2004 Mar 01;246:2373-84. doi: 10.1128/MCB.24.6.2373-2384.2004.
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Nup358/RanBP2 attaches to the nuclear pore complex via association with Nup88 and Nup214/CAN and plays a supporting role in CRM1-mediated nuclear protein export.
Bernad R
,
van der Velde H
,
Fornerod M
,
Pickersgill H
.
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Nuclear pore complexes (NPCs) traverse the nuclear envelope (NE), providing a channel through which nucleocytoplasmic transport occurs. Nup358/RanBP2, Nup214/CAN, and Nup88 are components of the cytoplasmic face of the NPC. Here we show that Nup88 localizes midway between Nup358 and Nup214 and physically interacts with them. RNA interference of either Nup88 or Nup214 in human cells caused a strong reduction of Nup358 at the NE. Nup88 and Nup214 showed an interdependence at the NPC and were not affected by the absence of Nup358. These data indicate that Nup88 and Nup214 mediate the attachment of Nup358 to the NPC. We show that localization of the export receptor CRM1 at the cytoplasmic face of the NE is Nup358 dependent and represents its empty state. Also, removal of Nup358 causes a distinct reduction in nuclear export signal-dependent nuclear export. We propose that Nup358 provides both a platform for rapid disassembly of CRM1 export complexes and a binding site for empty CRM1 recycling into the nucleus.
Adachi,
Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery.
1989, Pubmed
Adachi,
Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery.
1989,
Pubmed
Agami,
Distinct initiation and maintenance mechanisms cooperate to induce G1 cell cycle arrest in response to DNA damage.
2000,
Pubmed
Arts,
The role of exportin-t in selective nuclear export of mature tRNAs.
1998,
Pubmed
,
Xenbase
Askjaer,
RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase.
1999,
Pubmed
,
Xenbase
Bastos,
Targeting and function in mRNA export of nuclear pore complex protein Nup153.
1996,
Pubmed
Bastos,
Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex.
1997,
Pubmed
Becskei,
The strategy for coupling the RanGTP gradient to nuclear protein export.
2003,
Pubmed
,
Xenbase
Ben-Efraim,
Gradient of increasing affinity of importin beta for nucleoporins along the pathway of nuclear import.
2001,
Pubmed
Brummelkamp,
A system for stable expression of short interfering RNAs in mammalian cells.
2002,
Pubmed
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Davis,
Nuclear pore complex contains a family of glycoproteins that includes p62: glycosylation through a previously unidentified cellular pathway.
1987,
Pubmed
Delphin,
RanGTP targets p97 to RanBP2, a filamentous protein localized at the cytoplasmic periphery of the nuclear pore complex.
1997,
Pubmed
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Englmeier,
Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis.
1999,
Pubmed
Favreau,
Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210.
1996,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fornerod,
Relocation of the carboxyterminal part of CAN from the nuclear envelope to the nucleus as a result of leukemia-specific chromosome rearrangements.
1995,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Fornerod,
Interaction of cellular proteins with the leukemia specific fusion proteins DEK-CAN and SET-CAN and their normal counterpart, the nucleoporin CAN.
1996,
Pubmed
Fukuda,
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.
1997,
Pubmed
,
Xenbase
Goldberg,
The nuclear pore complex: three-dimensional surface structure revealed by field emission, in-lens scanning electron microscopy, with underlying structure uncovered by proteolysis.
1993,
Pubmed
,
Xenbase
Görlich,
Transport into and out of the cell nucleus.
1998,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Griffis,
Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes.
2003,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Hase,
Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex.
2003,
Pubmed
Henderson,
A comparison of the activity, sequence specificity, and CRM1-dependence of different nuclear export signals.
2000,
Pubmed
Hurwitz,
Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex.
1998,
Pubmed
Jarnik,
Toward a more complete 3-D structure of the nuclear pore complex.
1991,
Pubmed
,
Xenbase
Kaffman,
The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus.
1998,
Pubmed
Kasper,
CREB binding protein interacts with nucleoporin-specific FG repeats that activate transcription and mediate NUP98-HOXA9 oncogenicity.
1999,
Pubmed
Kehlenbach,
A role for RanBP1 in the release of CRM1 from the nuclear pore complex in a terminal step of nuclear export.
1999,
Pubmed
Kraemer,
The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.
1994,
Pubmed
Kudo,
Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.
1998,
Pubmed
Kudo,
Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins.
1997,
Pubmed
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Lipowsky,
Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes.
2000,
Pubmed
Macaulay,
Differential mitotic phosphorylation of proteins of the nuclear pore complex.
1995,
Pubmed
,
Xenbase
Mahajan,
A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2.
1997,
Pubmed
Matsuoka,
Identification and characterization of nuclear pore subcomplexes in mitotic extract of human somatic cells.
1999,
Pubmed
Matunis,
SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex.
1998,
Pubmed
Matunis,
A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex.
1996,
Pubmed
,
Xenbase
Miller,
Phosphorylation and glycosylation of nucleoporins.
1999,
Pubmed
,
Xenbase
Moroianu,
Previously identified protein of uncertain function is karyopherin alpha and together with karyopherin beta docks import substrate at nuclear pore complexes.
1995,
Pubmed
Nakielny,
Transport of proteins and RNAs in and out of the nucleus.
1999,
Pubmed
Neville,
The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae.
1999,
Pubmed
Ossareh-Nazari,
Evidence for a role of CRM1 in signal-mediated nuclear protein export.
1997,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Reichelt,
Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.
1990,
Pubmed
,
Xenbase
Ribbeck,
The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy.
1999,
Pubmed
Ribbeck,
Kinetic analysis of translocation through nuclear pore complexes.
2001,
Pubmed
Ris,
High-resolution field emission scanning electron microscope imaging of internal cell structures after Epon extraction from sections: a new approach to correlative ultrastructural and immunocytochemical studies.
1993,
Pubmed
,
Xenbase
Rout,
The nuclear pore complex as a transport machine.
2001,
Pubmed
Singh,
The zinc finger cluster domain of RanBP2 is a specific docking site for the nuclear export factor, exportin-1.
1999,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Ullman,
Nuclear export receptors: from importin to exportin.
1997,
Pubmed
Ullman,
The nucleoporin nup153 plays a critical role in multiple types of nuclear export.
1999,
Pubmed
,
Xenbase
van Deursen,
G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214.
1996,
Pubmed
Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Walther,
The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.
2002,
Pubmed
,
Xenbase
Walther,
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.
2001,
Pubmed
,
Xenbase
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
Wen,
Heat-stable inhibitors of cAMP-dependent protein kinase carry a nuclear export signal.
1994,
Pubmed
Wu,
Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region.
1995,
Pubmed
Yaseen,
Cloning and characterization of human karyopherin beta3.
1997,
Pubmed
Yokoyama,
A giant nucleopore protein that binds Ran/TC4.
1995,
Pubmed