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We have isolated a Xenopus homologue of the zinc finger/homeodomain-containing transcriptional repressor Smad-interacting protein-1 (SIP1) from mouse. XSIP1 is activated at the early gastrula stage and transcription occurs throughout embryogenesis. At the beginning of gastrulation, XSIP1 is strongly expressed in prospective neurectoderm. At the neurula stage, XSIP1 is highly expressed within the neural plate but weakly in the dorsal midline. At later stages of development transcripts are detected primarily within the neural tube and neural crest. In the adult, XSIP1 expression is detected at variable levels in several organs.
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10842070
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Fig. 2. Expression of XSIP1 during Xenopus development. (Top panels) RNase protection analysis of XSIP1 transcripts during embryogenesis and in dissected regions of stage 10.5 embryos compared with the expression of other genes. Abbreviations: E, eggs; A, animal pole; V, vegetal pole; M, marginal zone; DMZ, dorsal marginal zone; VMZ, ventral marginal zone. ODC (ornithine decarboxylase) is used as an internal control. (Bottom panels) Whole-mount in situ hybridization using XSIP1 antisense RNA. Nieuwkoop–Faber stages of embryogenesis are indicated. All embryos are shown with anterior region to the right. (A,C,E,G) Dorsal view; (D,H,I) lateral view. (B) Sagittal section of the embryo show in (A). (F) Transversal section of the embryo shown in (E). (J–L) Transversal sections of the embryo shown in I at the level of the trunk (J,K, high magnification) or hindbrain (L). (M) Transversal section at the level of the eyes of a stage 37 embryo. The arrowheads show the dorsal lip in (A) and the closing blastopore in (C–E). Abbreviations: bcs, branchial crest segment; cmz, ciliary marginal zone; dbl, dorsal blastopore lip; eln, epithelial layer of neurectoderm; hcs, hyoid crest segment; idm, involuting dorsal mesoderm; inl, inner nuclear layer; lpm, lateral plate mesoderm; mcs, mandibular crest segment; n, notochord; nc, neural crest; nt, neural tube; sc, spinal cord; sln, sensorial layer of neurectoderm.
