Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Housing and husbandry of Xenopus laevis affect the quality of oocytes for heterologous expression studies.
Delpire E
,
Gagnon KB
,
Ledford JJ
,
Wallace JM
.
???displayArticle.abstract???
To assess the effect of Xenopus husbandry on oocyte quality for membrane transport physiology experiments, we compared a recirculating-water housing system with a static-water system in a 23-mo study. Two groups of frogs (n = 8) were maintained separately for the entire study: one group was housed in a multiinvestigator centrally managed Xenopus facility, which consists of 33 tanks placed on a shared and recirculating water system; the other group was housed in a satellite facility used by a single investigator and consisting of static tanks placed in a dedicated cold-room. The activity of a heterologously expressed membrane transporter was assessed every 4 to 5 wk for a total of 23 mo. Activity of the mouse cotransporter NKCC1 was assessed through isotopic (86) Rb influx measurements under 2 experimental conditions: stimulation of cotransporter by coinjection of regulatory kinases and by exposure to a hypertonic solution. The results showed a significant difference in the level of ion fluxes under these 2 experimental conditions between the 2 groups of oocytes. During the entire period, oocytes isolated from frogs maintained in the static facility demonstrated consistently robust NKCC1 function, whereas oocytes isolated from frogs maintained in the recirculating facility showed inconsistent and weaker cotransporter function. Furthermore, the oocytes isolated from frogs maintained in the recirculating facility showed significant deterioration during the summer months (April to August), a seasonal variation that was muted in frog oocytes maintained in the static facility.
Brown,
Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.
1993, Pubmed,
Xenbase
Brown,
Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.
1993,
Pubmed
,
Xenbase
Brown,
A tribute to the Xenopus laevis oocyte and egg.
2004,
Pubmed
,
Xenbase
Delpire,
Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells.
1994,
Pubmed
Gagnon,
A single binding motif is required for SPAK activation of the Na-K-2Cl cotransporter.
2007,
Pubmed
,
Xenbase
Gagnon,
Volume sensitivity of cation-Cl- cotransporters is modulated by the interaction of two kinases: Ste20-related proline-alanine-rich kinase and WNK4.
2006,
Pubmed
,
Xenbase
Gagnon,
Multiple pathways for protein phosphatase 1 (PP1) regulation of Na-K-2Cl cotransporter (NKCC1) function: the N-terminal tail of the Na-K-2Cl cotransporter serves as a regulatory scaffold for Ste20-related proline/alanine-rich kinase (SPAK) AND PP1.
2010,
Pubmed
,
Xenbase
Gamba,
Primary structure and functional expression of a cDNA encoding the thiazide-sensitive, electroneutral sodium-chloride cotransporter.
1993,
Pubmed
,
Xenbase
Geck,
The Na-K-2Cl cotransport system.
1986,
Pubmed
Godfrey,
Effect of water hardness on oocyte quality and embryo development in the African clawed frog (Xenopus laevis).
2004,
Pubmed
,
Xenbase
Green,
Factors affecting oogenesis in the South African clawed frog (Xenopus laevis).
2002,
Pubmed
,
Xenbase
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Hilken,
Growth of Xenopus laevis under different laboratory rearing conditions.
1995,
Pubmed
,
Xenbase
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Jentsch,
Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Piechotta,
Cation chloride cotransporters interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1).
2002,
Pubmed
Piechotta,
Characterization of the interaction of the stress kinase SPAK with the Na+-K+-2Cl- cotransporter in the nervous system: evidence for a scaffolding role of the kinase.
2003,
Pubmed
,
Xenbase
Russell,
Sodium-potassium-chloride cotransport.
2000,
Pubmed
Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase
Strange,
Dependence of KCC2 K-Cl cotransporter activity on a conserved carboxy terminus tyrosine residue.
2000,
Pubmed
,
Xenbase
Straub,
Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.
1990,
Pubmed
,
Xenbase
Tanaka,
Expression cloning of a rat glutamate transporter.
1993,
Pubmed
,
Xenbase
Wu,
Raising Xenopus in the laboratory.
1991,
Pubmed
,
Xenbase
Yamashita,
Expression cloning of a cDNA encoding the bovine histamine H1 receptor.
1991,
Pubmed
,
Xenbase
