Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.
Reichelt R
,
Holzenburg A
,
Buhle EL
,
Jarnik M
,
Engel A
,
Aebi U
.
???displayArticle.abstract???
Nuclear pore complexes (NPCs) prepared from Xenopus laevis oocyte nuclear envelopes were studied in "intact" form (i.e., unexposed to detergent) and after detergent treatment by a combination of conventional transmission electron microscopy (CTEM) and quantitative scanning transmission electron microscopy (STEM). In correlation-averaged CTEM pictures of negatively stained intact NPCs and of distinct NPC components (i.e., "rings," "spoke" complexes, and "plug-spoke" complexes), several fine structural features arranged with octagonal symmetry about a central axis could reproducibly be identified. STEM micrographs of unstained/freeze-dried intact NPCs as well as of their components yielded comparable but less distinct features. Mass determination by STEM revealed the following molecular masses: intact NPC with plug, 124 +/- 11 MD; intact NPC without plug, 112 +/- 11 MD; heavy ring, 32 +/- 5 MD; light ring, 21 +/- 4 MD; plug-spoke complex, 66 +/- 8 MD; and spoke complex, 52 +/- 3 MD. Based on these combined CTEM and STEM data, a three-dimensional model of the NPC exhibiting eightfold centrosymmetry about an axis perpendicular to the plane of the nuclear envelope but asymmetric along this axis is proposed. This structural polarity of the NPC across the nuclear envelope is in accord with its well-documented functional polarity facilitating mediated nucleocytoplasmic exchange of molecules and particles.
Abelson,
Nuclear pores: the pore-annulus relationship in thin section.
1970, Pubmed
Abelson,
Nuclear pores: the pore-annulus relationship in thin section.
1970,
Pubmed
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase
Akey,
Interactions and structure of the nuclear pore complex revealed by cryo-electron microscopy.
1989,
Pubmed
,
Xenbase
Akey,
Protein import through the nuclear pore complex is a multistep process.
1989,
Pubmed
,
Xenbase
Blobel,
Gene gating: a hypothesis.
1985,
Pubmed
Bonner,
Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.
1975,
Pubmed
,
Xenbase
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Buhle,
Correlation of surface topography of metal-shadowed specimens with their negatively stained reconstructions.
1985,
Pubmed
,
Xenbase
Buhle,
The structure of the Ca2+ ATPase as revealed by electron microscopy and image processing of ordered arrays.
1983,
Pubmed
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase
Dwyer,
A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.
1976,
Pubmed
Engel,
Mass mapping of a protein complex with the scanning transmission electron microscope.
1982,
Pubmed
Engel,
Processing of quantitative scanning transmission electron micrographs.
1988,
Pubmed
Engel,
Molecular weight determination by scanning transmission electron microscopy.
1978,
Pubmed
Engel,
Preparation of unstained protein structures for mass determination by electron scattering.
1980,
Pubmed
Feldherr,
The uptake of endogenous proteins by oocyte nuclei.
1975,
Pubmed
,
Xenbase
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed
Franke,
Structure, biochemistry, and functions of the nuclear envelope.
1974,
Pubmed
Franke,
The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte.
1970,
Pubmed
Gall,
Octagonal nuclear pores.
1967,
Pubmed
Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed
Harris,
The biochemistry and ultrastructure of the nuclear envelope.
1978,
Pubmed
Harris,
Some electron microscopic studies on intact nuclear 'ghosts' and nuclear membrane fragments.
1974,
Pubmed
Hoeijmakers,
Structure of the nuclear pore complex in mammalian cells. Two annular components.
1974,
Pubmed
Kartenbeck,
The nuclear envelope in freeze-etching.
1971,
Pubmed
,
Xenbase
Krohne,
The major polypeptides of the nuclear pore complex.
1978,
Pubmed
,
Xenbase
Leapman,
Quantitative electron energy loss spectroscopy in biology.
1988,
Pubmed
Maul,
The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.
1977,
Pubmed
Newport,
The nucleus: structure, function, and dynamics.
1987,
Pubmed
Paine,
Nuclear envelope permeability.
1975,
Pubmed
Unwin,
A large particle associated with the perimeter of the nuclear pore complex.
1982,
Pubmed
,
Xenbase
Unwin,
Three-dimensional model of membrane-bound ribosomes obtained by electron microscopy.
1977,
Pubmed
Wrigley,
The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy.
1968,
Pubmed
Zimmer,
The function of the nuclear envelope in nuclear protein accumulation.
1988,
Pubmed
,
Xenbase
