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In mammals, resistance to primary and secondary viral infections critically involves major histocompatibility complex class I-restricted cytotoxic CD8+ T lymphocytes (CTLs). Although many gene homologues involved in CTL function have been identified in all vertebrate classes, antiviral CTL responses have been poorly characterized for ectothermic vertebrates. Because of the threat of emerging wildlife viral diseases to global biodiversity, fundamental research on comparative viral immunity has become crucial. Ranaviruses (family Iridoviridae) are double-stranded DNA viruses possibly implicated in the worldwide decline of amphibian populations. We used the frog Xenopus laevis as a model to evaluate adaptive immune responses to the ranavirus frog virus 3 (FV3). FV3 infects the kidneys of adults but is cleared within 4 weeks, with faster clearance upon secondary infections. In vivo depletion of CD8+ T cells markedly decreases the survival of adults after viral infection. To further investigate the involvement of anti-FV3 CD8+ T-cell effectors in host resistance in vivo, we determined the proliferation kinetics of CD8+ T cells in the spleen by bromodeoxyuridine incorporation and their infiltration of kidneys by immunohistology. Upon primary infection, CD8+ T cells significantly proliferate in the spleen and accumulate in infected kidneys from day 6 onward, in parallel with virus clearance. Earlier proliferation and infiltration associated with faster viral clearance were observed during a secondary infection. These results provide in vivo evidence of protective antigen-dependent CD8+ T-cell proliferation, recognition, and memory in fighting a natural pathogen in Xenopus.
Adler,
Cellular recognition by mouse lymphocytes in vitro. I. Definition of a new technique and results of stimulation by phytohemagglutinin and specific antigens.
1970, Pubmed
Adler,
Cellular recognition by mouse lymphocytes in vitro. I. Definition of a new technique and results of stimulation by phytohemagglutinin and specific antigens.
1970,
Pubmed
Alvarez,
Removal of PHA from supernatants containing T-cell growth factor.
1981,
Pubmed
Badovinac,
Programmed contraction of CD8(+) T cells after infection.
2002,
Pubmed
Baggiolini,
Chemokines and leukocyte traffic.
1998,
Pubmed
Bleicher,
Monoclonal anti-IgM can separate T cell from B cell proliferative responses in the frog, Xenopus laevis.
1981,
Pubmed
,
Xenbase
Buller,
Poxvirus pathogenesis.
1991,
Pubmed
Butcher,
Lymphocyte homing and homeostasis.
1996,
Pubmed
Cauley,
Renewal of peripheral CD8+ memory T cells during secondary viral infection of antibody-sufficient mice.
2003,
Pubmed
Chinchar,
Ranaviruses (family Iridoviridae): emerging cold-blooded killers.
2002,
Pubmed
Daszak,
Emerging infectious diseases and amphibian population declines.
1999,
Pubmed
Doherty,
Accessing complexity: the dynamics of virus-specific T cell responses.
2000,
Pubmed
Du Pasquier,
The immune system of Xenopus.
1989,
Pubmed
,
Xenbase
Du Pasquier,
B-cell development in the amphibian Xenopus.
2000,
Pubmed
,
Xenbase
Gantress,
Development and characterization of a model system to study amphibian immune responses to iridoviruses.
2003,
Pubmed
,
Xenbase
Garcia-Camacho,
Early cell-mediated immune responses to Marek's disease virus in two chicken lines with defined major histocompatibility complex antigens.
2003,
Pubmed
Hangartner,
Antiviral antibody responses: the two extremes of a wide spectrum.
2006,
Pubmed
Hashimoto,
Isolation of carp genes encoding major histocompatibility complex antigens.
1990,
Pubmed
Jürgens,
Identification of a candidate CD5 homologue in the amphibian Xenopus laevis.
1995,
Pubmed
,
Xenbase
Lallone,
In vitro correlates of in vivo skin graft rejection in Xenopus.
1985,
Pubmed
,
Xenbase
Lanzavecchia,
Understanding the generation and function of memory T cell subsets.
2005,
Pubmed
Litman,
Evolution of antigen binding receptors.
1999,
Pubmed
Maniero,
Generation of a long-lasting, protective, and neutralizing antibody response to the ranavirus FV3 by the frog Xenopus.
2006,
Pubmed
,
Xenbase
Maniero,
Phylogenetic conservation of gp96-mediated antigen-specific cellular immunity: new evidence from adoptive cell transfer in xenopus.
2004,
Pubmed
,
Xenbase
McCullough,
Basic concepts of immune response and defense development.
2005,
Pubmed
Panchanathan,
Interferon function is not required for recovery from a secondary poxvirus infection.
2005,
Pubmed
Rau,
Identification and characterization of Xenopus CD8+ T cells expressing an NK cell-associated molecule.
2002,
Pubmed
,
Xenbase
Robert,
Adaptive immunity and histopathology in frog virus 3-infected Xenopus.
2005,
Pubmed
,
Xenbase
Robert,
Minor histocompatibility antigen-specific MHC-restricted CD8 T cell responses elicited by heat shock proteins.
2002,
Pubmed
,
Xenbase
Somamoto,
Role of specific cell-mediated cytotoxicity in protecting fish from viral infections.
2002,
Pubmed
Takeuchi,
Cloning and characterization of class I Mhc genes of the zebrafish, Brachydanio rerio.
1995,
Pubmed
Watkins,
In vitro proliferative and cytotoxic responses against Xenopus minor histocompatibility antigens.
1988,
Pubmed
,
Xenbase
Wilson,
T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes.
1998,
Pubmed
Zhou,
Heterogeneity of channel catfish CTL with respect to target recognition and cytotoxic mechanisms employed.
2001,
Pubmed
Zinkernagel,
MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
1979,
Pubmed
