Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Molecular cloning and characterization of novel ficolins from Xenopus laevis.
Kakinuma Y
,
Endo Y
,
Takahashi M
,
Nakata M
,
Matsushita M
,
Takenoshita S
,
Fujita T
.
???displayArticle.abstract???
Ficolins are proteins characterized by the presence of collagen- and fibrinogen-like domains. Two of three human ficolins, L-ficolin and H-ficolin, are serum lectins and are thought to play crucial roles in host defense through opsonization and complement activation. To elucidate the evolution of ficolins and the primordial complement lectin pathway, we cloned four ficolin cDNAs from Xenopus laevis, termed Xenopus ficolin (XeFCN) 1, 2, 3 and 4. The deduced amino acid sequences of the four ficolins revealed the conserved collagen- and fibrinogen-like domains. The full sequences of the four ficolins showed a 42-56% identity to human ficolins, and 60-83% between one another. Northern blots showed that XeFCN1 was expressed mainly in liver, spleen and heart, and XeFCN2 and XeFCN4 mainly in peripheral blood leukocytes, lung and spleen. We isolated ficolin proteins from Xenopus serum by affinity chromatography on N-acetylglucosamine-agarose, followed by ion-exchange chromatography. The final eluate showed polymeric bands composed of two components of 37 and 40 kDa. The N-terminal amino acid sequences and treatment with endoglycosidase F showed that the two bands are the same XeFCN1 protein with different masses of N-linked sugar. The polymeric form of the two types of XeFCN1 specifically recognized GlcNAc and GalNAc residues. These results suggest that like human L-ficolin, XeFCN1 functions in the circulation through its lectin activity.
Akaiwa,
Hakata antigen, a new member of the ficolin/opsonin p35 family, is a novel human lectin secreted into bronchus/alveolus and bile.
1999, Pubmed
Akaiwa,
Hakata antigen, a new member of the ficolin/opsonin p35 family, is a novel human lectin secreted into bronchus/alveolus and bile.
1999,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Endo,
Two lineages of mannose-binding lectin-associated serine protease (MASP) in vertebrates.
1998,
Pubmed
,
Xenbase
Endo,
Cloning and characterization of the human lectin P35 gene and its related gene.
1996,
Pubmed
Epstein,
The collectins in innate immunity.
1996,
Pubmed
Fujimori,
Molecular cloning and characterization of mouse ficolin-A.
1998,
Pubmed
Gokudan,
Horseshoe crab acetyl group-recognizing lectins involved in innate immunity are structurally related to fibrinogen.
1999,
Pubmed
Ichijo,
Molecular cloning and characterization of ficolin, a multimeric protein with fibrinogen- and collagen-like domains.
1993,
Pubmed
Ichijo,
Purification of transforming growth factor-beta 1 binding proteins from porcine uterus membranes.
1991,
Pubmed
Kairies,
The 2.0-A crystal structure of tachylectin 5A provides evidence for the common origin of the innate immunity and the blood coagulation systems.
2001,
Pubmed
Kenjo,
Cloning and characterization of novel ficolins from the solitary ascidian, Halocynthia roretzi.
2001,
Pubmed
Le,
Human L-ficolin: plasma levels, sugar specificity, and assignment of its lectin activity to the fibrinogen-like (FBG) domain.
1998,
Pubmed
Lu,
Biosynthesis of human ficolin, an Escherichia coli-binding protein, by monocytes: comparison with the synthesis of two macrophage-specific proteins, C1q and the mannose receptor.
1996,
Pubmed
Lu,
Human ficolin: cDNA cloning, demonstration of peripheral blood leucocytes as the major site of synthesis and assignment of the gene to chromosome 9.
1996,
Pubmed
Matsushita,
Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease.
1992,
Pubmed
Matsushita,
A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin.
1996,
Pubmed
Matsushita,
Cutting edge: complement-activating complex of ficolin and mannose-binding lectin-associated serine protease.
2000,
Pubmed
Matsushita,
Activation of the lectin complement pathway by H-ficolin (Hakata antigen).
2002,
Pubmed
Ohashi,
Oligomeric structure and tissue distribution of ficolins from mouse, pig and human.
1998,
Pubmed
Okada,
A novel membrane glycoprotein capable of inhibiting membrane attack by homologous complement.
1989,
Pubmed
Omori-Satoh,
The antihemorrhagic factor, erinacin, from the European hedgehog (Erinaceus europaeus), a metalloprotease inhibitor of large molecular size possessing ficolin/opsonin P35 lectin domains.
2000,
Pubmed
Saitou,
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
1987,
Pubmed
Sato,
Molecular characterization of a novel serine protease involved in activation of the complement system by mannose-binding protein.
1994,
Pubmed
Sugimoto,
Cloning and characterization of the Hakata antigen, a member of the ficolin/opsonin p35 lectin family.
1998,
Pubmed
Takahashi,
A truncated form of mannose-binding lectin-associated serine protease (MASP)-2 expressed by alternative polyadenylation is a component of the lectin complement pathway.
1999,
Pubmed
Teh,
M-ficolin is expressed on monocytes and is a lectin binding to N-acetyl-D-glucosamine and mediates monocyte adhesion and phagocytosis of Escherichia coli.
2000,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Turner,
Mannose-binding lectin: the pluripotent molecule of the innate immune system.
1996,
Pubmed
Yae,
Isolation and characterization of a thermolabile beta-2 macroglycoprotein ('thermolabile substance' or 'Hakata antigen') detected by precipitating (auto) antibody in sera of patients with systemic lupus erythematosus.
1991,
Pubmed
