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Dynamic regulation of histone modifications in Xenopus oocytes through histone exchange.
Stewart MD
,
Sommerville J
,
Wong J
.
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Histone H3 lysine 9 (H3K9) methylation has broad roles in transcriptional repression, gene silencing, maintenance of heterochromatin, and epigenetic inheritance of heterochromatin. Using Xenopus laevis oocytes, we have previously shown that targeting G9a, an H3K9 histone methyltransferase, to chromatin increases H3K9 methylation and consequently represses transcription. Here we report that treatment with trichostatin A induces histone acetylation and is sufficient to activate transcription repressed by G9a, and this activation is accompanied by a reduction in dimethyl H3K9 (H3K9me2). We tested the possibility that the reduction in H3K9me2 was due to the replacement of methylated H3 with unmethylated H3.3. Surprisingly, we found that both free H3 and H3.3 are continually exchanged with chromatin-associated histones. This dynamic exchange of chromatin-associated H3 with free H3/H3.3 was not affected by alterations in transcriptional activity, elongation, acetylation, H3K9 methylation, or DNA replication. In support of this continual histone exchange model, we show that maintenance of H3K9 methylation at a specific site requires the continual presence of an H3K9 histone methyltransferase. Upon dissociation of the methyltransferase, H3K9 methylation decreases. Taken together, our data suggest that chromatin-associated and non-chromatin-associated histones are continually exchanged in the Xenopus oocyte, creating a highly dynamic chromatin environment.
Ahmad,
The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
2002, Pubmed
Ahmad,
The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly.
2002,
Pubmed
Ahmad,
Epigenetic consequences of nucleosome dynamics.
2002,
Pubmed
Ahmad,
Histone H3 variants specify modes of chromatin assembly.
2002,
Pubmed
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Bannister,
Reversing histone methylation.
2005,
Pubmed
Bannister,
Histone methylation: recognizing the methyl mark.
2004,
Pubmed
Bannister,
Histone methylation: dynamic or static?
2002,
Pubmed
Becker,
Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila embryos.
1992,
Pubmed
Berger,
Histone modifications in transcriptional regulation.
2002,
Pubmed
Boeger,
Removal of promoter nucleosomes by disassembly rather than sliding in vivo.
2004,
Pubmed
Boeger,
Nucleosomes unfold completely at a transcriptionally active promoter.
2003,
Pubmed
Bulger,
Assembly of regularly spaced nucleosome arrays by Drosophila chromatin assembly factor 1 and a 56-kDa histone-binding protein.
1995,
Pubmed
Byvoet,
The distribution and turnover of labeled methyl groups in histone fractions of cultured mammalian cells.
1972,
Pubmed
Cuthbert,
Histone deimination antagonizes arginine methylation.
2004,
Pubmed
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Ehrenhofer-Murray,
Chromatin dynamics at DNA replication, transcription and repair.
2004,
Pubmed
Ercan,
Global nucleosome distribution and the regulation of transcription in yeast.
2004,
Pubmed
Gelius,
Characterization of a chromatin remodelling activity in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Groth,
Human Asf1 regulates the flow of S phase histones during replicational stress.
2005,
Pubmed
Guschin,
Multiple ISWI ATPase complexes from xenopus laevis. Functional conservation of an ACF/CHRAC homolog.
2000,
Pubmed
,
Xenbase
Honda,
Histone methylation. Its occurrence in different cell types and relation to histone H4 metabolism in developing trout testis.
1975,
Pubmed
Janicki,
From silencing to gene expression: real-time analysis in single cells.
2004,
Pubmed
Jenuwein,
Translating the histone code.
2001,
Pubmed
Jin,
In and out: histone variant exchange in chromatin.
2005,
Pubmed
Khorasanizadeh,
The nucleosome: from genomic organization to genomic regulation.
2004,
Pubmed
Kikyo,
Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI.
2000,
Pubmed
,
Xenbase
Kimura,
Histone dynamics in living cells revealed by photobleaching.
2005,
Pubmed
Kimura,
Kinetics of core histones in living human cells: little exchange of H3 and H4 and some rapid exchange of H2B.
2001,
Pubmed
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Korber,
Evidence for histone eviction in trans upon induction of the yeast PHO5 promoter.
2004,
Pubmed
Lachner,
An epigenetic road map for histone lysine methylation.
2003,
Pubmed
Lee,
Evidence for nucleosome depletion at active regulatory regions genome-wide.
2004,
Pubmed
Li,
Involvement of histone methylation and phosphorylation in regulation of transcription by thyroid hormone receptor.
2002,
Pubmed
,
Xenbase
Maison,
Higher-order structure in pericentric heterochromatin involves a distinct pattern of histone modification and an RNA component.
2002,
Pubmed
Martin,
The diverse functions of histone lysine methylation.
2005,
Pubmed
Metzger,
LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
2005,
Pubmed
Mito,
Genome-scale profiling of histone H3.3 replacement patterns.
2005,
Pubmed
Mizuguchi,
ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
2004,
Pubmed
Peters,
Histone H3 lysine 9 methylation is an epigenetic imprint of facultative heterochromatin.
2002,
Pubmed
Peters,
Partitioning and plasticity of repressive histone methylation states in mammalian chromatin.
2003,
Pubmed
Peterson,
Histones and histone modifications.
2004,
Pubmed
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Reinke,
Histones are first hyperacetylated and then lose contact with the activated PHO5 promoter.
2003,
Pubmed
Richardson,
Characterization of the histone H1-binding protein, NASP, as a cell cycle-regulated somatic protein.
2000,
Pubmed
,
Xenbase
Saccani,
Dynamic changes in histone H3 Lys 9 methylation occurring at tightly regulated inducible inflammatory genes.
2002,
Pubmed
Santos-Rosa,
Active genes are tri-methylated at K4 of histone H3.
2002,
Pubmed
Schneider,
Histone H3 lysine 4 methylation patterns in higher eukaryotic genes.
2004,
Pubmed
Schwabish,
Evidence for eviction and rapid deposition of histones upon transcriptional elongation by RNA polymerase II.
2004,
Pubmed
Schwartz,
Transcriptional activation triggers deposition and removal of the histone variant H3.3.
2005,
Pubmed
Shi,
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
2004,
Pubmed
Sittman,
Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.
1983,
Pubmed
Smillie,
Nuclear import and activity of histone deacetylase in Xenopus oocytes is regulated by phosphorylation.
2004,
Pubmed
,
Xenbase
Sommerville,
Signal recognition particle assembly in relation to the function of amplified nucleoli of Xenopus oocytes.
2005,
Pubmed
,
Xenbase
Stewart,
Relationship between histone H3 lysine 9 methylation, transcription repression, and heterochromatin protein 1 recruitment.
2005,
Pubmed
Tagami,
Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis.
2004,
Pubmed
Thiriet,
Replication-independent core histone dynamics at transcriptionally active loci in vivo.
2005,
Pubmed
,
Xenbase
Tsukada,
Histone demethylation by a family of JmjC domain-containing proteins.
2006,
Pubmed
Vidanes,
Complicated tails: histone modifications and the DNA damage response.
2005,
Pubmed
Wells,
Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.
1985,
Pubmed
Whetstine,
Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases.
2006,
Pubmed
Wong,
Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase.
1998,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Wu,
Patterns of histone variant synthesis can distinguish G0 from G1 cells.
1982,
Pubmed
Yamane,
JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor.
2006,
Pubmed
