Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Rouxs Arch Dev Biol
1989 May 01;1981:8-13. doi: 10.1007/BF00376364.
Show Gene links
Show Anatomy links
A mesoderm-inducing factor from a Xenopus laevis cell line : Chemical properties and relation to the vegetalizing factor from chicken embryos.
Grunz H
,
Born J
,
Davids M
,
Hoppe P
,
Loppnow-Blinde B
,
Tacke L
,
Tiedemann H
,
Tiedemann H
.
???displayArticle.abstract???
We have compared the chemical properties and biological activities of the mesoderm-inducing factor that is secreted by the Xenopus XTC cell line with the vegetalizing factor from chicken embryos. The inducing activity of the factors was tested in different concentrations on totipotent ectoderm either by implantation into early gastrulae of Triturm alpestris or by application of solutions to isolated ectoderm of early gastrulae of Xenopus laevis. Both factors have similar properties. They are not irreversibly inactivated after treatment with 6 M urea or with phenol at 60° C. Reduction with thioglycolic acid inactivates the factors completely. The inducing activity of XTC-conditioned medium decreases only slightly after treatment with 50% formic acid. The apparent molecular mass and the isoelectric point of the factors are similar. The XTC factor was partially purified by size-exclusion and reversed-phase high-pressure liquid chromatography and by isoelectric focusing. The possible relationship of these factors to transforming growth factor β is discussed.
Asahi,
Formation of mesodermal pattern by secondary inducing interactions.
1979, Pubmed
Asahi,
Formation of mesodermal pattern by secondary inducing interactions.
1979,
Pubmed
Asashima,
Inducing effects of the presumptive endoderm of successive stages inTriturus alpestris.
1975,
Pubmed
BECKER,
[Experiments on the determination of embryonal amphibian tissue by induction substances in solution].
1959,
Pubmed
Born,
Affinity chromatography of embryonic inducing factors on heparin-Sepharose.
1987,
Pubmed
Born,
An embryonic inducing factor: isolation by high performance liquid chromatography and chemical properties.
1985,
Pubmed
Born,
Isolation of a vegetalizing inducing factor.
1972,
Pubmed
Boterenbrood,
The formation of the mesoderm in urodelean amphibians : V. Its regional induction by the endoderm.
1973,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Geithe,
A vegetalizing inducing factor. Isolation and chemical properties.
1981,
Pubmed
Geithe,
Electrofocusing of the vegetalizing inducing factor.
1970,
Pubmed
Godsave,
Mesoderm-inducing factors: a small class of molecules.
1988,
Pubmed
,
Xenbase
Grunz,
The inducing capacity of the presumptive endoderm of Xenopus laevis studied by transfilter experiments.
1986,
Pubmed
,
Xenbase
Grunz,
Induction of mesodermal tissues by acidic and basic heparin binding growth factors.
1988,
Pubmed
,
Xenbase
Janeczek,
Inducing activity of subcellular fractions from amphibian embryos.
1984,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Knöchel,
Mesoderm-inducing factors. Their possible relationship to heparin-binding growth factors and transforming growth factor-beta.
1987,
Pubmed
KOCHER-BECKER,
EXOVAGINATION OF NEWT ENDODERM: CELL AFFINITIES ALTERED BY THE MESODERMAL INDUCING FACTOR.
1965,
Pubmed
Landon,
Cleavage at aspartyl-prolyl bonds.
1977,
Pubmed
Lobb,
Purification of heparin-binding growth factors.
1986,
Pubmed
Minuth,
The formation of mesodermal derivatives after induction with vegetalizing factor depends on secondary cell interactions.
1980,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Pudney,
Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.
1973,
Pubmed
,
Xenbase
Roberts,
Purification and properties of a type beta transforming growth factor from bovine kidney.
1983,
Pubmed
Rosa,
Mesoderm induction in amphibians: the role of TGF-beta 2-like factors.
1988,
Pubmed
,
Xenbase
Schwarz,
High performance gel permeation chromatography of proteins. Application to embryonic inducing factors.
1981,
Pubmed
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Smith,
A mesoderm-inducing factor is produced by Xenopus cell line.
1987,
Pubmed
,
Xenbase
Smith,
Purification, partial characterization and biological effects of the XTC mesoderm-inducing factor.
1988,
Pubmed
,
Xenbase
Tiedemann,
Test of embryonic inducing factors: advantages and disadvantages of different procedures.
1986,
Pubmed
Tiedemann,
[Action of sulfhydryl compounds on embryonic inducing factors].
1969,
Pubmed
TIEDEMANN,
[Isolation of ribonucleic acids and nucleotides from embryonal extracts and liver, and their inductor behavior].
1956,
Pubmed
TIEDEMANN,
[Experiments on the chemical identification of embryonal induction substances].
1956,
Pubmed
TOIVONEN,
The simultaneous inducing action of liver and bone-marrow of the guinea-pig in implantation and explantation experiments with embryos of Triturus.
1955,
Pubmed
YAMADA,
A technique for testing macromolecular samples in solution for morphogenetic effects on the isolated ectoderm of the amphibian gastrula.
1961,
Pubmed
