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XB-ART-22319
Cell 1993 Aug 13;743:555-63.
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Protein kinase C zeta isoform is critical for mitogenic signal transduction.

Berra E , Diaz-Meco MT , Dominguez I , Municio MM , Sanz L , Lozano J , Chapkin RS , Moscat J .


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The requirement of protein kinase C zeta (zeta PKC) for maturation of X. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase C has recently been shown. Here we present experimental evidence demonstrating that activation of zeta PKC is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. Furthermore, by using a dominant kinase-defective mutant of zeta PKC, we confirm that this kinase is required for mitogenic activation in oocytes and fibroblasts. These results permit us to propose zeta PKC as a critical step downstream of p21ras in mitogenic signal transduction.

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Species referenced: Xenopus laevis
Genes referenced: ins