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Proc Natl Acad Sci U S A
1996 Jun 25;9313:6343-8. doi: 10.1073/pnas.93.13.6343.
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Cystic fibrosis gene encodes a cAMP-dependent chloride channel in heart.
Hart P
,
Warth JD
,
Levesque PC
,
Collier ML
,
Geary Y
,
Horowitz B
,
Hume JR
.
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cAMP-dependent chloride channels in heart contribute to autonomic regulation of action potential duration and membrane potential and have been inferred to be due to cardiac expression of the epithelial cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. In this report, a cDNA from rabbit ventricle was isolated and sequenced, which encodes an exon 5 splice variant (exon 5-) of CFTR, with >90% identity to human CFTR cDNA present in epithelial cells. Expression of this cDNA in Xenopus oocytes gave rise to robust cAMP-activated chloride currents that were absent in control water-injected oocytes. Antisense oligodeoxynucleotides directed against CFTR significantly reduced the density of cAMP-dependent chloride currents in acutely cultured myocytes, thereby establishing a direct functional link between cardiac expression of CFTR protein and an endogenous chloride channel in native cardiac myocytes.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
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Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Bahinski,
Chloride conductance regulated by cyclic AMP-dependent protein kinase in cardiac myocytes.
1989,
Pubmed
Baukrowitz,
Coupling of CFTR Cl- channel gating to an ATP hydrolysis cycle.
1994,
Pubmed
Collier,
Unitary chloride channels activated by protein kinase C in guinea pig ventricular myocytes.
1995,
Pubmed
Delaney,
Cystic fibrosis transmembrane conductance regulator splice variants are not conserved and fail to produce chloride channels.
1993,
Pubmed
Duan,
Sustained outward current observed after I(to1) inactivation in rabbit atrial myocytes is a novel Cl- current.
1992,
Pubmed
Ehara,
Anion channels activated by adrenaline in cardiac myocytes.
1990,
Pubmed
Gadsby,
The CFTR chloride channel of mammalian heart.
1995,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Harvey,
Chloride current in mammalian cardiac myocytes. Novel mechanism for autonomic regulation of action potential duration and resting membrane potential.
1990,
Pubmed
Harvey,
Autonomic regulation of a chloride current in heart.
1989,
Pubmed
Horowitz,
Alternative splicing of CFTR Cl- channels in heart.
1993,
Pubmed
Hume,
A plethora of cardiac chloride conductances: molecular diversity or a related gene family.
1995,
Pubmed
Hume,
Ionic basis of the different action potential configurations of single guinea-pig atrial and ventricular myocytes.
1985,
Pubmed
Hwang,
Regulation of the gating of cystic fibrosis transmembrane conductance regulator C1 channels by phosphorylation and ATP hydrolysis.
1994,
Pubmed
Kopelman,
Antisense oligodeoxynucleotide to the cystic fibrosis transmembrane conductance regulator inhibits cyclic AMP-activated but not calcium-activated cell volume reduction in a human pancreatic duct cell line.
1993,
Pubmed
Kowdley,
Hyperpolarization-activated chloride currents in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Krauss,
Antisense oligonucleotides to CFTR confer a cystic fibrosis phenotype on B lymphocytes.
1992,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Levesque,
Expression of cystic fibrosis transmembrane regulator Cl- channels in heart.
1992,
Pubmed
,
Xenbase
Levesque,
Anion and cation modulation of the guinea-pig ventricular action potential during beta-adrenoceptor stimulation.
1993,
Pubmed
Levesque,
ATPo but not cAMPi activates a chloride conductance in mouse ventricular myocytes.
1995,
Pubmed
Matsuura,
Activation of chloride current by purinergic stimulation in guinea pig heart cells.
1992,
Pubmed
Moorman,
Phospholemman expression induces a hyperpolarization-activated chloride current in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Nagel,
The protein kinase A-regulated cardiac Cl- channel resembles the cystic fibrosis transmembrane conductance regulator.
1992,
Pubmed
Overholt,
On the mechanism of rectification of the isoproterenol-activated chloride current in guinea-pig ventricular myocytes.
1993,
Pubmed
Paulmichl,
New mammalian chloride channel identified by expression cloning.
1992,
Pubmed
,
Xenbase
Rommens,
Identification of the cystic fibrosis gene: chromosome walking and jumping.
1989,
Pubmed
Sipido,
[Ca2+]i transients and [Ca2+]i-dependent chloride current in single Purkinje cells from rabbit heart.
1993,
Pubmed
Sorota,
Swelling-induced chloride-sensitive current in canine atrial cells revealed by whole-cell patch-clamp method.
1992,
Pubmed
Sorscher,
Antisense oligodeoxynucleotide to the cystic fibrosis gene inhibits anion transport in normal cultured sweat duct cells.
1991,
Pubmed
Thiemann,
A chloride channel widely expressed in epithelial and non-epithelial cells.
1992,
Pubmed
,
Xenbase
Tseng,
Cell swelling increases membrane conductance of canine cardiac cells: evidence for a volume-sensitive Cl channel.
1992,
Pubmed
Walsh,
Activation of a heart chloride current during stimulation of protein kinase C.
1991,
Pubmed
Warth,
CFTR chloride channels in human and simian heart.
1996,
Pubmed
Zhang,
Protein kinase-dependent Cl- currents in feline ventricular myocytes.
1994,
Pubmed
Zygmunt,
Calcium-activated chloride current in rabbit ventricular myocytes.
1991,
Pubmed