Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2008 Jun 13;28324:16928-39. doi: 10.1074/jbc.M801454200.
Show Gene links
Show Anatomy links
PATE gene clusters code for multiple, secreted TFP/Ly-6/uPAR proteins that are expressed in reproductive and neuron-rich tissues and possess neuromodulatory activity.
Levitin F
,
Weiss M
,
Hahn Y
,
Stern O
,
Papke RL
,
Matusik R
,
Nandana SR
,
Ziv R
,
Pichinuk E
,
Salame S
,
Bera T
,
Vincent J
,
Lee B
,
Pastan I
,
Wreschner DH
.
???displayArticle.abstract???
We report here syntenic loci in humans and mice incorporating gene clusters coding for secreted proteins each comprising 10 cysteine residues. These conform to three-fingered protein/Ly-6/urokinase-type plasminogen activator receptor (uPAR) domains that shape three-fingered proteins (TFPs). The founding gene is PATE, expressed primarily in prostate and less in testis. We have identified additional human PATE-like genes (PATE-M, PATE-DJ, and PATE-B) that co-localize with the PATE locus, code for novel secreted PATE-like proteins, and show selective expression in prostate and/or testis. Anti-PATE-B-specific antibodies demonstrated the presence of PATE-B in the region of the sperm acrosome and at high levels on malignant prostatic epithelial cells. The syntenic mouse Pate-like locus encompasses 14 active genes coding for secreted proteins, which are all, except for Pate-P and Pate-Q, expressed primarily in prostate and/or testis. Pate-P and Pate-Q are expressed solely in placental tissue. Castration up-regulates prostate expression of mouse Pate-B and Pate-E, whereas testosterone ablates this induced expression. The sequence similarity between TFP/Ly-6/uPAR proteins that modulate activity of nicotinic acetylcholine receptors and the PATE (Pate)-like proteins stimulated us to see whether these proteins possess analogous activity. Pharmacological studies showed significant modulation of the nicotinic acetylcholines by the PATE-B, Pate-C, and Pate-P proteins. In concert with these findings, certain PATE (Pate)-like genes were extensively expressed in neuron-rich tissues. Taken together, our findings indicate that in addition to participation of the PATE (Pate)-like genes in functions related to fertility and reproduction, some of them likely act as important modulators of neural transmission.
Adermann,
Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein superfamily.
1999, Pubmed
Adermann,
Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein superfamily.
1999,
Pubmed
Arredondo,
SLURP-2: A novel cholinergic signaling peptide in human mucocutaneous epithelium.
2006,
Pubmed
Bera,
PATE, a gene expressed in prostate cancer, normal prostate, and testis, identified by a functional genomic approach.
2002,
Pubmed
Bray,
Acetylcholine causes an increase of intracellular calcium in human sperm.
2005,
Pubmed
Bray,
Mice deficient in CHRNA7, a subunit of the nicotinic acetylcholine receptor, produce sperm with impaired motility.
2005,
Pubmed
Bray,
A nicotinic acetylcholine receptor is involved in the arosome reaction of human sperm initiated by recombinant human ZP3.
2002,
Pubmed
Chimienti,
Identification of SLURP-1 as an epidermal neuromodulator explains the clinical phenotype of Mal de Meleda.
2003,
Pubmed
,
Xenbase
Coronel,
Purification, structure, and characterization of caltrin proteins from seminal vesicle of the rat and mouse.
1992,
Pubmed
Foster,
Human SP-10: acrosomal distribution, processing, and fate after the acrosome reaction.
1994,
Pubmed
Freemerman,
Characterization of alternatively spliced human SP-10 mRNAs.
1995,
Pubmed
Fry,
Molecular evolution and phylogeny of elapid snake venom three-finger toxins.
2003,
Pubmed
Herr,
Identification of human acrosomal antigen SP-10 in primates and pigs.
1990,
Pubmed
Ibañez-Tallon,
Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1.
2002,
Pubmed
,
Xenbase
Kolbe,
Xenoxins, a family of peptides from dorsal gland secretion of Xenopus laevis related to snake venom cytotoxins and neurotoxins.
1993,
Pubmed
,
Xenbase
Kumar,
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment.
2004,
Pubmed
Kumar,
Nicotinic acetylcholine receptor subunits and associated proteins in human sperm.
2005,
Pubmed
Levitin,
The MUC1 SEA module is a self-cleaving domain.
2005,
Pubmed
Luo,
A novel heat-labile phospholipid-binding protein, SVS VII, in mouse seminal vesicle as a sperm motility enhancer.
2001,
Pubmed
Meizel,
The sperm, a neuron with a tail: 'neuronal' receptors in mammalian sperm.
2004,
Pubmed
Ménez,
Functional architectures of animal toxins: a clue to drug design?
1998,
Pubmed
Nirthanan,
Three-finger alpha-neurotoxins and the nicotinic acetylcholine receptor, forty years on.
2004,
Pubmed
Notredame,
T-Coffee: A novel method for fast and accurate multiple sequence alignment.
2000,
Pubmed
Papke,
Comparative pharmacology of rat and human alpha7 nAChR conducted with net charge analysis.
2002,
Pubmed
,
Xenbase
Ploug,
Structure-function relationships in the receptor for urokinase-type plasminogen activator. Comparison to other members of the Ly-6 family and snake venom alpha-neurotoxins.
1994,
Pubmed
Ploug,
Structure-function relationships in the interaction between the urokinase-type plasminogen activator and its receptor.
2003,
Pubmed
Satomi,
N-glycosylation at Asn(491) in the Asn-Xaa-Cys motif of human transferrin.
2004,
Pubmed
Soler-García,
The PATE gene is expressed in the accessory tissues of the human male genital tract and encodes a secreted sperm-associated protein.
2005,
Pubmed
Son,
Evidence suggesting that the mouse sperm acrosome reaction initiated by the zona pellucida involves an alpha7 nicotinic acetylcholine receptor.
2003,
Pubmed
Southan,
The characterisation of novel secreted Ly-6 proteins from rat urine by the combined use of two-dimensional gel electrophoresis, microbore high performance liquid chromatography and expressed sequence tag data.
2002,
Pubmed
Stokes,
The structural basis for GTS-21 selectivity between human and rat nicotinic alpha7 receptors.
2004,
Pubmed
,
Xenbase
Trémeau,
Genetic engineering of snake toxins. The functional site of Erabutoxin a, as delineated by site-directed mutagenesis, includes variant residues.
1995,
Pubmed
Tsetlin,
Snake venom alpha-neurotoxins and other 'three-finger' proteins.
1999,
Pubmed
Tsetlin,
Snake and snail toxins acting on nicotinic acetylcholine receptors: fundamental aspects and medical applications.
2004,
Pubmed
Tsuji,
SLURP-2, a novel member of the human Ly-6 superfamily that is up-regulated in psoriasis vulgaris.
2003,
Pubmed
