Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Planta
2005 Jun 01;2213:328-38. doi: 10.1007/s00425-004-1446-8.
Show Gene links
Show Anatomy links
Amino acid contents and transport in oilseed rape (Brassica napus L.) under different nitrogen conditions.
Tilsner J
,
Kassner N
,
Struck C
,
Lohaus G
.
???displayArticle.abstract???
Oilseed rape (Brassica napus L.) needs very high nitrogen fertilizer inputs. Significant amounts of this nitrogen are lost during early leaf shedding and are a source of environmental and economic concern. The objective of this study was to investigate whether the remobilization of leaf amino acids could be limiting for nitrogen use efficiency. Therefore, amino acid concentrations were analyzed in subcellular compartments of leaf mesophyll cells of plants grown under low (0.5 mM NO3-) and high (4 mM NO3-) nitrogen supply. With high nitrogen supply, young leaves showed an elevated amino acid content, mainly in vacuoles. In old leaves, however, subcellular concentrations were similar under high and low nitrogen conditions, showing that the excess nitrogen had been exported during leaf development. The phloem sap contained up to 650 mM amino acids, more than four times as much than the cytosol of mesophyll cells, indicating a very efficient phloem-loading process. Three amino acid permeases, BnAAP1, BnAAP2, and BnAAP6, were identified and characterized. BnAAP1 and BnAAP6 mediated uptake of neutral and acidic amino acids into Xenopus laevis oocytes at the actual apoplastic substrate concentrations. All three transporters were expressed in leaves and the expression was still detectable during leaf senescence, with BnAAP1 and BnAAP2 mRNA levels increasing from mature to old leaves. We conclude that phloem loading of amino acids is not limiting for nitrogen remobilization from senescing leaves in oilseed rape.
Bhalerao,
Gene expression in autumn leaves.
2003, Pubmed
Bhalerao,
Gene expression in autumn leaves.
2003,
Pubmed
Boorer,
Specificity and stoichiometry of the Arabidopsis H+/amino acid transporter AAP5.
1997,
Pubmed
,
Xenbase
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Dietrich,
AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis.
2004,
Pubmed
,
Xenbase
Fischer,
Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids.
2002,
Pubmed
,
Xenbase
Fischer,
Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis.
1995,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Frommer,
Expression cloning in yeast of a cDNA encoding a broad specificity amino acid permease from Arabidopsis thaliana.
1993,
Pubmed
Gan,
Inhibition of leaf senescence by autoregulated production of cytokinin.
1995,
Pubmed
Gepstein,
Large-scale identification of leaf senescence-associated genes.
2003,
Pubmed
Gerhardt,
Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media.
1984,
Pubmed
Hanahan,
Studies on transformation of Escherichia coli with plasmids.
1983,
Pubmed
Handa,
The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana.
2003,
Pubmed
Haritatos,
Minor vein structure and sugar transport in Arabidopsis thaliana.
2000,
Pubmed
Hirner,
Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.
1998,
Pubmed
Jefferson,
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
1987,
Pubmed
Kwart,
Differential expression of two related amino acid transporters with differing substrate specificity in Arabidopsis thaliana.
1993,
Pubmed
Lohaus,
Is the infiltration-centrifugation technique appropriate for the isolation of apoplastic fluid? A critical evaluation with different plant species.
2001,
Pubmed
Lohaus,
Phloem transport of amino acids in two Brassica napus L. genotypes and one B. carinata genotype in relation to their seed protein content.
2000,
Pubmed
Miller,
Xenopus oocytes as an expression system for plant transporters.
2000,
Pubmed
,
Xenbase
Miranda,
Amino acid permeases in developing seeds of Vicia faba L.: expression precedes storage protein synthesis and is regulated by amino acid supply.
2001,
Pubmed
Noh,
Regulation of developmental senescence is conserved between Arabidopsis and Brassica napus.
1999,
Pubmed
Okumoto,
High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis.
2002,
Pubmed
Ortiz-Lopez,
Amino acid transporters in plants.
2000,
Pubmed
Rathgeber,
Nucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba).
1990,
Pubmed
Rentsch,
Salt stress-induced proline transporters and salt stress-repressed broad specificity amino acid permeases identified by suppression of a yeast amino acid permease-targeting mutant.
1996,
Pubmed
Riens,
Amino Acid and sucrose content determined in the cytosolic, chloroplastic, and vacuolar compartments and in the Phloem sap of spinach leaves.
1991,
Pubmed
Rossato,
Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns.
2001,
Pubmed
Stacey,
Peptide transport in plants.
2002,
Pubmed
Struck,
The Uromyces fabae UfAAT3 gene encodes a general amino acid permease that prefers uptake of in planta scarce amino acids.
2004,
Pubmed
,
Xenbase
Tegeder,
Amino acid transporters are localized to transfer cells of developing pea seeds.
2000,
Pubmed
Unfried,
Nucleotide sequence of the 18S rRNA gene from Arabidopsis thaliana Co10.
1989,
Pubmed
Wipf,
Conservation of amino acid transporters in fungi, plants and animals.
2002,
Pubmed
