Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Physiol
2009 Mar 01;587Pt 5:929-52. doi: 10.1113/jphysiol.2008.164756.
Show Gene links
Show Anatomy links
Intracellular traffic of the K+ channels TASK-1 and TASK-3: role of N- and C-terminal sorting signals and interaction with 14-3-3 proteins.
Zuzarte M
,
Heusser K
,
Renigunta V
,
Schlichthörl G
,
Rinné S
,
Wischmeyer E
,
Daut J
,
Schwappach B
,
Preisig-Müller R
.
???displayArticle.abstract???
The two-pore-domain potassium channels TASK-1 (KCNK3) and TASK-3 (KCNK9) modulate the electrical activity of neurons and many other cell types. We expressed TASK-1, TASK-3 and related reporter constructs in Xenopus oocytes, mammalian cell lines and various yeast strains to study the mechanisms controlling their transport to the surface membrane and the role of 14-3-3 proteins. We measured potassium currents with the voltage-clamp technique and fused N- and C-terminal fragments of the channels to various reporter proteins to study changes in subcellular localisation and surface expression. Mutational analysis showed that binding of 14-3-3 proteins to the extreme C-terminus of TASK-1 and TASK-3 masks a tri-basic motif, KRR, which differs in several important aspects from canonical arginine-based (RxR) or lysine-based (KKxx) retention signals. Pulldown experiments with GST fusion proteins showed that the KRR motif in the C-terminus of TASK-3 channels was able to bind to COPI coatomer. Disabling the binding of 14-3-3, which exposes the KRR motif, caused localisation of the GFP-tagged channel protein mainly to the Golgi complex. TASK-1 and TASK-3 also possess a di-basic N-terminal retention signal, KR, whose function was found to be independent of the binding of 14-3-3. Suppression of channel surface expression with dominant-negative channel mutants revealed that interaction with 14-3-3 has no significant effect on the dimeric assembly of the channels. Our results give a comprehensive description of the mechanisms by which 14-3-3 proteins, together with N- and C-terminal sorting signals, control the intracellular traffic of TASK-1 and TASK-3.
Aitken,
14-3-3 proteins: a historic overview.
2006, Pubmed
Aitken,
14-3-3 proteins: a historic overview.
2006,
Pubmed
Allouis,
14-3-3 is a regulator of the cardiac voltage-gated sodium channel Nav1.5.
2006,
Pubmed
Barr,
GRASP65, a protein involved in the stacking of Golgi cisternae.
1997,
Pubmed
Bayliss,
Emerging roles for two-pore-domain potassium channels and their potential therapeutic impact.
2008,
Pubmed
Boyle,
Selective export of HLA-F by its cytoplasmic tail.
2006,
Pubmed
Brickley,
TASK-3 two-pore domain potassium channels enable sustained high-frequency firing in cerebellar granule neurons.
2007,
Pubmed
Buckler,
TASK-like potassium channels and oxygen sensing in the carotid body.
2007,
Pubmed
Catterall,
Regulation of sodium and calcium channels by signaling complexes.
2006,
Pubmed
Coblitz,
C-terminal recognition by 14-3-3 proteins for surface expression of membrane receptors.
2005,
Pubmed
Cooper,
Characterization and function of TWIK-related acid sensing K+ channels in a rat nociceptive cell.
2004,
Pubmed
Cui,
Deafness associated changes in two-pore domain potassium channels in the rat inferior colliculus.
2007,
Pubmed
Czirják,
TASK-3 dominates the background potassium conductance in rat adrenal glomerulosa cells.
2002,
Pubmed
,
Xenbase
Czirják,
Phosphorylation-dependent binding of 14-3-3 proteins controls TRESK regulation.
2008,
Pubmed
,
Xenbase
Davies,
TASK channel deletion in mice causes primary hyperaldosteronism.
2008,
Pubmed
Decressac,
ARF6-dependent interaction of the TWIK1 K+ channel with EFA6, a GDP/GTP exchange factor for ARF6.
2004,
Pubmed
Dougherty,
Unlocking the code of 14-3-3.
2004,
Pubmed
Duprat,
TASK, a human background K+ channel to sense external pH variations near physiological pH.
1997,
Pubmed
,
Xenbase
Eugster,
The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs.
2004,
Pubmed
Girard,
p11, an annexin II subunit, an auxiliary protein associated with the background K+ channel, TASK-1.
2002,
Pubmed
Gu,
The microtubule plus-end tracking protein EB1 is required for Kv1 voltage-gated K+ channel axonal targeting.
2006,
Pubmed
Heitzmann,
Invalidation of TASK1 potassium channels disrupts adrenal gland zonation and mineralocorticoid homeostasis.
2008,
Pubmed
Heusser,
Scavenging of 14-3-3 proteins reveals their involvement in the cell-surface transport of ATP-sensitive K+ channels.
2006,
Pubmed
,
Xenbase
Jackson,
Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
1990,
Pubmed
Kagan,
14-3-3 amplifies and prolongs adrenergic stimulation of HERG K+ channel activity.
2002,
Pubmed
Keith,
Bidirectional modulation of transmitter release by calcium channel/syntaxin interactions in vivo.
2007,
Pubmed
,
Xenbase
Kim,
Up-regulated astroglial TWIK-related acid-sensitive K+ channel-1 (TASK-1) in the hippocampus of seizure-sensitive gerbils: a target of anti-epileptic drugs.
2007,
Pubmed
Kim,
TASK-3, a new member of the tandem pore K(+) channel family.
2000,
Pubmed
Li,
Voltage-gated potassium channels: regulation by accessory subunits.
2006,
Pubmed
Li,
Modulation of inactivation properties of CaV2.2 channels by 14-3-3 proteins.
2006,
Pubmed
Linden,
TASK-3 knockout mice exhibit exaggerated nocturnal activity, impairments in cognitive functions, and reduced sensitivity to inhalation anesthetics.
2007,
Pubmed
Lopes,
Block of Kcnk3 by protons. Evidence that 2-P-domain potassium channel subunits function as homodimers.
2001,
Pubmed
,
Xenbase
Lopes,
Proton block and voltage gating are potassium-dependent in the cardiac leak channel Kcnk3.
2000,
Pubmed
,
Xenbase
Mackintosh,
Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes.
2004,
Pubmed
Margeta-Mitrovic,
A trafficking checkpoint controls GABA(B) receptor heterodimerization.
2000,
Pubmed
,
Xenbase
Mathie,
Neuronal two-pore-domain potassium channels and their regulation by G protein-coupled receptors.
2007,
Pubmed
Meuth,
Contribution of TWIK-related acid-sensitive K+ channel 1 (TASK1) and TASK3 channels to the control of activity modes in thalamocortical neurons.
2003,
Pubmed
Michelsen,
A multimeric membrane protein reveals 14-3-3 isoform specificity in forward transport in yeast.
2006,
Pubmed
Michelsen,
Hide and run. Arginine-based endoplasmic-reticulum-sorting motifs in the assembly of heteromultimeric membrane proteins.
2005,
Pubmed
Michelsen,
Novel cargo-binding site in the beta and delta subunits of coatomer.
2007,
Pubmed
Mrowiec,
14-3-3 proteins in membrane protein transport.
2006,
Pubmed
Mulkey,
TASK channels determine pH sensitivity in select respiratory neurons but do not contribute to central respiratory chemosensitivity.
2007,
Pubmed
Nilsson,
Short cytoplasmic sequences serve as retention signals for transmembrane proteins in the endoplasmic reticulum.
1989,
Pubmed
Nufer,
Role of cytoplasmic C-terminal amino acids of membrane proteins in ER export.
2002,
Pubmed
O'Kelly,
Forward transport. 14-3-3 binding overcomes retention in endoplasmic reticulum by dibasic signals.
2002,
Pubmed
O'Kelly,
Forward Transport of K2p3.1: mediation by 14-3-3 and COPI, modulation by p11.
2008,
Pubmed
,
Xenbase
Olschewski,
Impact of TASK-1 in human pulmonary artery smooth muscle cells.
2006,
Pubmed
Patel,
2P domain K+ channels: novel pharmacological targets for volatile general anesthetics.
2003,
Pubmed
Paulhe,
A specific endoplasmic reticulum export signal drives transport of stem cell factor (Kitl) to the cell surface.
2004,
Pubmed
Porter,
Dynamic 14-3-3/client protein interactions integrate survival and apoptotic pathways.
2006,
Pubmed
Putzke,
The acid-sensitive potassium channel TASK-1 in rat cardiac muscle.
2007,
Pubmed
,
Xenbase
Rajan,
TASK-3, a novel tandem pore domain acid-sensitive K+ channel. An extracellular histiding as pH sensor.
2000,
Pubmed
,
Xenbase
Rajan,
Interaction with 14-3-3 proteins promotes functional expression of the potassium channels TASK-1 and TASK-3.
2002,
Pubmed
,
Xenbase
Rau,
Expression of TWIK-related acid sensitive K+ channels in capsaicin sensitive and insensitive cells of rat dorsal root ganglia.
2006,
Pubmed
Renigunta,
The retention factor p11 confers an endoplasmic reticulum-localization signal to the potassium channel TASK-1.
2006,
Pubmed
,
Xenbase
Rescher,
S100A10/p11: family, friends and functions.
2008,
Pubmed
Sandoz,
Mtap2 is a constituent of the protein network that regulates twik-related K+ channel expression and trafficking.
2008,
Pubmed
,
Xenbase
Schutze,
An N-terminal double-arginine motif maintains type II membrane proteins in the endoplasmic reticulum.
1994,
Pubmed
Shikano,
Genetic isolation of transport signals directing cell surface expression.
2005,
Pubmed
Singer-Lahat,
Direct interaction of endogenous Kv channels with syntaxin enhances exocytosis by neuroendocrine cells.
2008,
Pubmed
Vacher,
Localization and targeting of voltage-dependent ion channels in mammalian central neurons.
2008,
Pubmed
Yuan,
14-3-3 dimers probe the assembly status of multimeric membrane proteins.
2003,
Pubmed
Zanzouri,
The background K(+) channel TASK-3 is regulated at both the transcriptional and post-transcriptional levels.
2006,
Pubmed
Zanzouri,
Membrane potential-regulated transcription of the resting K+ conductance TASK-3 via the calcineurin pathway.
2006,
Pubmed
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
Zuzarte,
A di-acidic sequence motif enhances the surface expression of the potassium channel TASK-3.
2007,
Pubmed
,
Xenbase
