Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell
2007 Mar 09;1285:1003-12. doi: 10.1016/j.cell.2006.12.041.
Show Gene links
Show Anatomy links
The site-specific installation of methyl-lysine analogs into recombinant histones.
Simon MD
,
Chu F
,
Racki LR
,
de la Cruz CC
,
Burlingame AL
,
Panning B
,
Narlikar GJ
,
Shokat KM
.
???displayArticle.abstract???
Histone lysine residues can be mono-, di-, or trimethylated. These posttranslational modifications regulate the affinity of effector proteins and may also impact chromatin structure independent of their role as adaptors. In order to study histone lysine methylation, particularly in the context of chromatin, we have developed a chemical approach to install analogs of methyl lysine into recombinant proteins. This approach allows for the rapid generation of large quantities of histones in which the site and degree of methylation can be specified. We demonstrate that these methyl-lysine analogs (MLAs) are functionally similar to their natural counterparts. These methylated histones were used to examine the influence of specific lysine methylation on the binding of effecter proteins and the rates of nucleosome remodeling. This simple method of introducing site-specific and degree-specific methylation into recombinant histones provides a powerful tool to investigate the biochemical mechanisms by which lysine methylation influences chromatin structure and function.
Chu,
Mapping post-translational modifications of the histone variant MacroH2A1 using tandem mass spectrometry.
2006, Pubmed
Chu,
Mapping post-translational modifications of the histone variant MacroH2A1 using tandem mass spectrometry.
2006,
Pubmed
Clapier,
Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI.
2001,
Pubmed
,
Xenbase
Clapier,
A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWI.
2002,
Pubmed
Cosgrove,
Regulated nucleosome mobility and the histone code.
2004,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Eberharter,
Acf1, the largest subunit of CHRAC, regulates ISWI-induced nucleosome remodelling.
2001,
Pubmed
Fischle,
Molecular basis for the discrimination of repressive methyl-lysine marks in histone H3 by Polycomb and HP1 chromodomains.
2003,
Pubmed
Gloss,
Decreasing the basicity of the active site base, Lys-258, of Escherichia coli aspartate aminotransferase by replacement with gamma-thialysine.
1995,
Pubmed
Grewal,
Heterochromatin and epigenetic control of gene expression.
2003,
Pubmed
Hamiche,
Histone tails modulate nucleosome mobility and regulate ATP-dependent nucleosome sliding by NURF.
2001,
Pubmed
He,
Facile synthesis of site-specifically acetylated and methylated histone proteins: reagents for evaluation of the histone code hypothesis.
2003,
Pubmed
,
Xenbase
He,
Human ACF1 alters the remodeling strategy of SNF2h.
2006,
Pubmed
Hopkins,
Aminoethylation in model peptides reveals conditions for maximizing thiol specificity.
2005,
Pubmed
Itano,
4-Thialaminine, a strongly basic chemical modification of cysteine.
1972,
Pubmed
Ivanova,
The chromo and SET domains of the Clr4 protein are essential for silencing in fission yeast.
1998,
Pubmed
Jacobs,
Structure of HP1 chromodomain bound to a lysine 9-methylated histone H3 tail.
2002,
Pubmed
Kenyon,
Novel sulfhydryl reagents.
1977,
Pubmed
Lachner,
An epigenetic road map for histone lysine methylation.
2003,
Pubmed
Lee,
Role of protein methylation in regulation of transcription.
2005,
Pubmed
Li,
Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF.
2006,
Pubmed
Lindroth,
Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3.
2004,
Pubmed
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Martin,
The diverse functions of histone lysine methylation.
2005,
Pubmed
Means,
Reductive alkylation of amino groups in proteins.
1968,
Pubmed
Min,
Structural basis for specific binding of Polycomb chromodomain to histone H3 methylated at Lys 27.
2003,
Pubmed
Muralidharan,
Protein ligation: an enabling technology for the biophysical analysis of proteins.
2006,
Pubmed
Nakayama,
Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly.
2001,
Pubmed
Nekrasov,
Nucleosome binding and histone methyltransferase activity of Drosophila PRC2.
2005,
Pubmed
,
Xenbase
Peña,
Molecular mechanism of histone H3K4me3 recognition by plant homeodomain of ING2.
2006,
Pubmed
Raftery,
On the aminoethylation of proteins.
1966,
Pubmed
Rea,
Regulation of chromatin structure by site-specific histone H3 methyltransferases.
2000,
Pubmed
Santos-Rosa,
Active genes are tri-methylated at K4 of histone H3.
2002,
Pubmed
Shi,
ING2 PHD domain links histone H3 lysine 4 methylation to active gene repression.
2006,
Pubmed
Shogren-Knaak,
A native peptide ligation strategy for deciphering nucleosomal histone modifications.
2003,
Pubmed
,
Xenbase
Shogren-Knaak,
Histone H4-K16 acetylation controls chromatin structure and protein interactions.
2006,
Pubmed
Shogren-Knaak,
Creating designer histones by native chemical ligation.
2004,
Pubmed
,
Xenbase
Sims,
Histone lysine methylation: a signature for chromatin function.
2003,
Pubmed
Strohner,
A 'loop recapture' mechanism for ACF-dependent nucleosome remodeling.
2005,
Pubmed
Tamaru,
Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa.
2003,
Pubmed
Turner,
Reading signals on the nucleosome with a new nomenclature for modified histones.
2005,
Pubmed
Wysocka,
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling.
2006,
Pubmed
,
Xenbase
Yang,
The chromatin-remodeling enzyme ACF is an ATP-dependent DNA length sensor that regulates nucleosome spacing.
2006,
Pubmed
