Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Mol Neurosci
1996 Jan 01;74:269-76. doi: 10.1007/BF02737064.
Show Gene links
Show Anatomy links
Deletion of the N-terminus of a K+ channel brings about short-term modulation by cAMP and beta 1-adrenergic receptor activation.
Levin G
,
Peretz T
,
Chikvashvilli D
,
Jing J
,
Lotan I
.
???displayArticle.abstract???
On deletion of the N-terminus of RCK1 K+ channel, acute modulation of the channel by cAMP-elevating treatments is revealed. This modulation is studied in Xenopus oocytes using two-electrode voltage-clamp, site-directed mutagenesis, and SDS-PAGE analyses. Treatments by Sp-8-Br-cAMPS, a membrane-permeant cAMP analog, and by isoproterenol, a beta 1-adrenergic receptor (beta 1R) agonist, both increased the current amplitudes with no effect on the voltage dependency of activation. The effect of isoproterenol was blocked by coexpression of either G alpha S or G alpha i3 proteins. The channel protein is phosphorylated on the Sp-8-Br-cAMPS treatment at Ser446; however, a phosphorylation-deficient variant in which this site has been altered is still modulated by Sp-8-Br-cAMPS and isoproterenol. Expression of the full-length channel with Kv beta 1.1 auxiliary subunit renders the channel at the same modulation as that of the truncated one. Taken together, the RCK1 channel can be acutely modulated by cAMP and beta 1R activation possibly through protein kinase A (PKA) activation, but not through direct channel phosphorylation; the involvement of the N-terminus in this modulation is discussed.
Blumenthal,
Modulation by cAMP of a slowly activating potassium channel expressed in Xenopus oocytes.
1992, Pubmed,
Xenbase
Blumenthal,
Modulation by cAMP of a slowly activating potassium channel expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Blumenthal,
The minK potassium channel exists in functional and nonfunctional forms when expressed in the plasma membrane of Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Busch,
An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C.
1992,
Pubmed
,
Xenbase
Covarrubias,
Elimination of rapid potassium channel inactivation by phosphorylation of the inactivation gate.
1994,
Pubmed
,
Xenbase
Crow,
Cellular and molecular analysis of associative learning and memory in Hermissenda.
1988,
Pubmed
Drain,
Regulation of Shaker K+ channel inactivation gating by the cAMP-dependent protein kinase.
1994,
Pubmed
,
Xenbase
Gilman,
G proteins: transducers of receptor-generated signals.
1987,
Pubmed
Holmes,
Tyrosine phosphorylation of the Kv1.3 potassium channel.
1996,
Pubmed
Huang,
Molecular basis of cardiac potassium channel stimulation by protein kinase A.
1994,
Pubmed
,
Xenbase
Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed
Ivanina,
Phosphorylation by protein kinase A of RCK1 K+ channels expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Levin,
Regulation of RCK1 currents with a cAMP analog via enhanced protein synthesis and direct channel phosphorylation.
1995,
Pubmed
,
Xenbase
Lim,
A G protein-gated K channel is activated via beta 2-adrenergic receptors and G beta gamma subunits in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Peretz,
Modulation by protein kinase C activation of rat brain delayed-rectifier K+ channel expressed in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Rehm,
Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I.
1988,
Pubmed
Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase
Rudy,
Diversity and ubiquity of K channels.
1988,
Pubmed
Scott,
Antibodies specific for distinct Kv subunits unveil a heterooligomeric basis for subtypes of alpha-dendrotoxin-sensitive K+ channels in bovine brain.
1994,
Pubmed
Sewing,
Kv beta 1 subunit binding specific for shaker-related potassium channel alpha subunits.
1996,
Pubmed
Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase
Stühmer,
Structure-function studies of voltage-gated ion channels.
1991,
Pubmed
Yu,
NAB domain is essential for the subunit assembly of both alpha-alpha and alpha-beta complexes of shaker-like potassium channels.
1996,
Pubmed
