Vernon AE and Philpott A (2003), The developmental expression of cell cycle regu...

XB-ART-5390

Gene Expr Patterns 2003 May 01;32:179-92. doi: 10.1016/s1567-133x(03)00006-1.

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The developmental expression of cell cycle regulators in Xenopus laevis.

Vernon AE , Philpott A .

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Several cyclins and cdks have been cloned in Xenopus, but their developmental expression has not been thoroughly examined. We have analyzed the temporal and spatial expression of cdk1, cdk2, cdk4 and cyclins D1, D2, E, A1, A2 and B1 by in situ hybridization. The transcripts of these cyclins and cdks exhibit striking tissue-restricted expression patterns very early in development that cannot be strictly correlated with proliferation. While the cdks and their activating cyclins are expressed in somewhat overlapping patterns, they are not precisely coincident. Additionally, maternal and zygotic cyclin forms demonstrate markedly different expression patterns.

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Species referenced: Xenopus laevis
Genes referenced: ccna1 ccna2 ccnb1 ccnd1 ccnd2 ccne1 cdk1 cdk2 cdk4

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	Fig. 1. Cyclin D1 expression. Whole-mount in situ hybridization for cyclin D1 expression at the indicated stages. (A) Lateral view with animal pole up. (B) View from dorsal side with blastopore down. (C,I) Dorsal views with anterior down. (D) Random orientation indicating dorsal cyclin D1 enrichment. (Eâ€“H,K,M) Anterior views. (J,N,O) Lateral views with anterior left. (L) Dorsal view with anterior left.
	Fig. 2. Cyclin D2 expression. Whole-mount in situ hybridization for cyclin D2 expression at the indicated stages. (A,B) Lateral views with animal pole up. (C,D,G,K,N) Anterior views. (E,H,I,L,M) Lateral views with anterior left. (F,J) Dorsal views with anterior left. (O) Ventral view with anterior left.
	Fig. 3. Cdk4 expression. Whole-mount in situ hybridization of cdk4 expression at the indicated stages. (A,B) Lateral view with animal pole up. (C,E,H) Dorsal views with anterior down. (D,G,J,L) Anterior views. (F,I) Posterior views. (K,N,O) Side view with anterior left. (M) Dorsal view with anterior left.
	Fig. 4. Cyclin E expression. Cyclin E expression demonstrated by whole-mount in situ hybridization at the indicated stages. (A) Lateral view with animal pole up. (B) Comparison of cyclin E expression levels at stages 8 and 10. (C,F) Dorsal views with anterior down. (D,E,G,I) Anterior views. (H,K,L) Lateral views with anterior left. (J) Dorsal view with anterior left.
	Fig. 5. Cdk2 expression. Analysis of cdk2 expression by whole-mount in situ hybridization at the indicated stages. (A,C) Random orientations demonstrating cdk2 expression in the animal pole. (B) Lateral view with animal pole up. (D,H) Dorsal views with anterior down. (E,F,G,J,L) Anterior views. (I,K,N,O) Lateral views with anterior left. (M) Dorsal view with anterior left.
	Fig. 6. Cyclin A1 expression. Analysis of cyclin A1 expression by whole-mount in situ hybridization at the indicated stages. (A,C) Lateral view with animal pole up. (B) Random orientation demonstrating cyclin A1 expression in the animal pole. (D) Dorsal view with anterior down. (E) Anterior view. (Fâ€“H) Lateral views with anterior to left.
	Fig. 7. Cyclin A2 expression. Cyclin A2 expression analyzed by whole-mount in situ hybridization at the indicated stages. (A,C) Lateral views with animal pole up. (B) Comparison of cyclin A2 expression levels at stages 8 and 10. (D,F,H,J) Dorsal views with anterior down. (E,G,I,K) Anterior views. (L,N) Dorsal views with anterior left. (M,O) Lateral views with anterior left.
	Fig. 8. Cyclin B1 expression. Cyclin B1 expression demonstrated by whole-mount in situ hybridization at the indicated stages. (A,B) Lateral views with animal pole up. (C,E,G,J) Dorsal views with anterior down. (D,F,H,I,K) Anterior views. (Lâ€“O) Lateral views with anterior left.
	Fig. 9. Cdk1 expression. Cdk1 expression by whole-mount in situ hybridization at the indicated stages. (A,B) Lateral views with animal pole up. (C) Dorsal view with anterior down. (Dâ€“F,H,K) Anterior views. (G,J,M,N) Lateral views with anterior left. (I,L) Dorsal views with anterior left.