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J Biol Chem
2015 Nov 06;29045:27176-27187. doi: 10.1074/jbc.M115.666305.
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Retinal Attachment Instability Is Diversified among Mammalian Melanopsins.
Tsukamoto H
,
Kubo Y
,
Farrens DL
,
Koyanagi M
,
Terakita A
,
Furutani Y
.
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Melanopsins play a key role in non-visual photoreception in mammals. Their close phylogenetic relationship to the photopigments in invertebrate visual cells suggests they have evolved to acquire molecular characteristics that are more suited for their non-visual functions. Here we set out to identify such characteristics by comparing the molecular properties of mammalian melanopsin to those of invertebrate melanopsin and visual pigment. Our data show that the Schiff base linking the chromophore retinal to the protein is more susceptive to spontaneous cleavage in mammalian melanopsins. We also find this stability is highly diversified between mammalian species, being particularly unstable for human melanopsin. Through mutagenesis analyses, we find that this diversified stability is mainly due to parallel amino acid substitutions in extracellular regions. We propose that the different stability of the retinal attachment in melanopsins may contribute to functional tuning of non-visual photoreception in mammals.
Ala-Laurila,
The action of 11-cis-retinol on cone opsins and intact cone photoreceptors.
2009, Pubmed
Ala-Laurila,
The action of 11-cis-retinol on cone opsins and intact cone photoreceptors.
2009,
Pubmed
Bailes,
Human melanopsin forms a pigment maximally sensitive to blue light (λmax ≈ 479 nm) supporting activation of G(q/11) and G(i/o) signalling cascades.
2013,
Pubmed
Berson,
Phototransduction by retinal ganglion cells that set the circadian clock.
2002,
Pubmed
Cameron,
β-Arrestin-dependent deactivation of mouse melanopsin.
2014,
Pubmed
Chew,
Loss of gq/11 genes does not abolish melanopsin phototransduction.
2014,
Pubmed
Cohen,
Constitutive activation of opsin: influence of charge at position 134 and size at position 296.
1993,
Pubmed
Dacey,
Melanopsin-expressing ganglion cells in primate retina signal colour and irradiance and project to the LGN.
2005,
Pubmed
Davies,
Functional diversity of melanopsins and their global expression in the teleost retina.
2011,
Pubmed
Do,
Intrinsically photosensitive retinal ganglion cells.
2010,
Pubmed
Do,
Photon capture and signalling by melanopsin retinal ganglion cells.
2009,
Pubmed
Doyle,
Nonvisual light responses in the Rpe65 knockout mouse: rod loss restores sensitivity to the melanopsin system.
2006,
Pubmed
Emanuel,
Melanopsin tristability for sustained and broadband phototransduction.
2015,
Pubmed
Fahrenkrug,
Phosphorylation of rat melanopsin at Ser-381 and Ser-398 by light/dark and its importance for intrinsically photosensitive ganglion cells (ipRGCs) cellular Ca2+ signaling.
2014,
Pubmed
Ferrer,
Dissecting the determinants of light sensitivity in amphioxus microvillar photoreceptors: possible evolutionary implications for melanopsin signaling.
2012,
Pubmed
Fu,
Intrinsically photosensitive retinal ganglion cells detect light with a vitamin A-based photopigment, melanopsin.
2005,
Pubmed
Fujiwara,
Ser165 in the second transmembrane region of the Kir2.1 channel determines its susceptibility to blockade by intracellular Mg2+.
2002,
Pubmed
,
Xenbase
Gamlin,
Human and macaque pupil responses driven by melanopsin-containing retinal ganglion cells.
2007,
Pubmed
Graham,
Melanopsin ganglion cells use a membrane-associated rhabdomeric phototransduction cascade.
2008,
Pubmed
Gross,
Characterization of rhodopsin congenital night blindness mutant T94I.
2003,
Pubmed
Hattar,
Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity.
2002,
Pubmed
Hughes,
Using siRNA to define functional interactions between melanopsin and multiple G Protein partners.
2015,
Pubmed
Isoldi,
Rhabdomeric phototransduction initiated by the vertebrate photopigment melanopsin.
2005,
Pubmed
,
Xenbase
Jacobs,
Losses of functional opsin genes, short-wavelength cone photopigments, and color vision--a significant trend in the evolution of mammalian vision.
2013,
Pubmed
Janz,
Role of the retinal hydrogen bond network in rhodopsin Schiff base stability and hydrolysis.
2004,
Pubmed
Janz,
Stability of dark state rhodopsin is mediated by a conserved ion pair in intradiscal loop E-2.
2003,
Pubmed
Jin,
Opsin activation as a cause of congenital night blindness.
2003,
Pubmed
,
Xenbase
Jones,
Small-molecule antagonists of melanopsin-mediated phototransduction.
2013,
Pubmed
Kawamura,
Ancestral loss of short wave-sensitive cone visual pigment in lorisiform prosimians, contrasting with its strict conservation in other prosimians.
2004,
Pubmed
Kawate,
Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins.
2006,
Pubmed
Kefalov,
Breaking the covalent bond--a pigment property that contributes to desensitization in cones.
2005,
Pubmed
Kono,
Constitutive activity of a UV cone opsin.
2006,
Pubmed
Koyanagi,
Jellyfish vision starts with cAMP signaling mediated by opsin-G(s) cascade.
2008,
Pubmed
Koyanagi,
Cephalochordate melanopsin: evolutionary linkage between invertebrate visual cells and vertebrate photosensitive retinal ganglion cells.
2005,
Pubmed
Koyanagi,
Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis- and all-trans-retinals as their chromophores.
2002,
Pubmed
Koyanagi,
Gq-coupled rhodopsin subfamily composed of invertebrate visual pigment and melanopsin.
2008,
Pubmed
Koyanagi,
Homologs of vertebrate Opn3 potentially serve as a light sensor in nonphotoreceptive tissue.
2013,
Pubmed
Kubo,
Structural basis for a Ca2+-sensing function of the metabotropic glutamate receptors.
1998,
Pubmed
,
Xenbase
Lucas,
Chromophore regeneration: melanopsin does its own thing.
2006,
Pubmed
Matsuyama,
Photochemical properties of mammalian melanopsin.
2012,
Pubmed
Mawad,
Absence of long-wavelength photic potentiation of murine intrinsically photosensitive retinal ganglion cell firing in vitro.
2008,
Pubmed
Melia,
A comparison of the efficiency of G protein activation by ligand-free and light-activated forms of rhodopsin.
1997,
Pubmed
Melyan,
Addition of human melanopsin renders mammalian cells photoresponsive.
2005,
Pubmed
Murakami,
Crystal structure of squid rhodopsin.
2008,
Pubmed
Mure,
Melanopsin-dependent nonvisual responses: evidence for photopigment bistability in vivo.
2007,
Pubmed
Nagata,
Depth perception from image defocus in a jumping spider.
2012,
Pubmed
Nakane,
A mammalian neural tissue opsin (Opsin 5) is a deep brain photoreceptor in birds.
2010,
Pubmed
,
Xenbase
Newman,
Melanopsin forms a functional short-wavelength photopigment.
2003,
Pubmed
Panda,
Illumination of the melanopsin signaling pathway.
2005,
Pubmed
,
Xenbase
Pires,
Differential expression of two distinct functional isoforms of melanopsin (Opn4) in the mammalian retina.
2009,
Pubmed
Provencio,
Melanopsin: An opsin in melanophores, brain, and eye.
1998,
Pubmed
,
Xenbase
Provencio,
A novel human opsin in the inner retina.
2000,
Pubmed
Qiu,
Induction of photosensitivity by heterologous expression of melanopsin.
2005,
Pubmed
Ramon,
Studies on the stability of the human cone visual pigments.
2009,
Pubmed
Ramon,
Unusual thermal and conformational properties of the rhodopsin congenital night blindness mutant Thr-94 --> Ile.
2003,
Pubmed
Rasmussen,
Crystal structure of the β2 adrenergic receptor-Gs protein complex.
2011,
Pubmed
Sakmar,
Glutamic acid-113 serves as the retinylidene Schiff base counterion in bovine rhodopsin.
1989,
Pubmed
Schmidt,
Intrinsically photosensitive retinal ganglion cells: many subtypes, diverse functions.
2011,
Pubmed
Schmidt,
Functional and morphological differences among intrinsically photosensitive retinal ganglion cells.
2009,
Pubmed
Tamura,
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
2013,
Pubmed
Terakita,
The opsins.
2005,
Pubmed
Terakita,
Expression and comparative characterization of Gq-coupled invertebrate visual pigments and melanopsin.
2008,
Pubmed
Torii,
Two isoforms of chicken melanopsins show blue light sensitivity.
2007,
Pubmed
Travis,
DISCO! Dissociation of cone opsins: the fast and noisy life of cones explained.
2005,
Pubmed
Tsukamoto,
A constitutively activating mutation alters the dynamics and energetics of a key conformational change in a ligand-free G protein-coupled receptor.
2013,
Pubmed
Tsukamoto,
The magnitude of the light-induced conformational change in different rhodopsins correlates with their ability to activate G proteins.
2009,
Pubmed
Tu,
Physiologic diversity and development of intrinsically photosensitive retinal ganglion cells.
2005,
Pubmed
Tu,
Inner retinal photoreception independent of the visual retinoid cycle.
2006,
Pubmed
Walker,
Photochemistry of retinal chromophore in mouse melanopsin.
2008,
Pubmed
