Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cleavage of cohesin rings coordinates the separation of centrioles and chromatids.
Schöckel L
,
Möckel M
,
Mayer B
,
Boos D
,
Stemmann O
.
???displayArticle.abstract???
Cohesin pairs sister chromatids by forming a tripartite Scc1-Smc1-Smc3 ring around them. In mitosis, cohesin is removed from chromosome arms by the phosphorylation-dependent prophase pathway. Centromeric cohesin is protected by shugoshin 1 and protein phosphatase 2A (Sgo1-PP2A) and opened only in anaphase by separase-dependent cleavage of Scc1 (refs 4-6). Following chromosome segregation, centrioles loosen their tight orthogonal arrangement, which licenses later centrosome duplication in S phase. Although a role of separase in centriole disengagement has been reported, the molecular details of this process remain enigmatic. Here, we identify cohesin as a centriole-engagement factor. Both premature sister-chromatid separation and centriole disengagement are induced by ectopic activation of separase or depletion of Sgo1. These unscheduled events are suppressed by expression of non-cleavable Scc1 or inhibition of the prophase pathway. When endogenous Scc1 is replaced by artificially cleavable Scc1, the corresponding site-specific protease triggers centriole disengagement. Separation of centrioles can alternatively be induced by ectopic cleavage of an engineered Smc3. Thus, the chromosome and centrosome cycles exhibit extensive parallels and are coordinated with each other by dual use of the cohesin ring complex.
Beauchene,
Rad21 is required for centrosome integrity in human cells independently of its role in chromosome cohesion.
2010, Pubmed
Beauchene,
Rad21 is required for centrosome integrity in human cells independently of its role in chromosome cohesion.
2010,
Pubmed
Boos,
Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase.
2008,
Pubmed
Bornens,
Studying the composition and function of centrosomes in vertebrates.
1999,
Pubmed
Bornkamm,
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system.
2005,
Pubmed
Cordingley,
Substrate requirements of human rhinovirus 3C protease for peptide cleavage in vitro.
1990,
Pubmed
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Faragher,
Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles.
2003,
Pubmed
Gandhi,
Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.
2006,
Pubmed
Ganem,
A mechanism linking extra centrosomes to chromosomal instability.
2009,
Pubmed
Giménez-Abián,
Regulation of sister chromatid cohesion between chromosome arms.
2004,
Pubmed
Gorr,
Mutual inhibition of separase and Cdk1 by two-step complex formation.
2005,
Pubmed
,
Xenbase
Gregson,
A potential role for human cohesin in mitotic spindle aster assembly.
2001,
Pubmed
Gruber,
Chromosomal cohesin forms a ring.
2003,
Pubmed
Guan,
Cohesin protein SMC1 is a centrosomal protein.
2008,
Pubmed
Haering,
The cohesin ring concatenates sister DNA molecules.
2008,
Pubmed
Hauf,
Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2.
2005,
Pubmed
Holland,
Cyclin-B1-mediated inhibition of excess separase is required for timely chromosome disjunction.
2006,
Pubmed
,
Xenbase
Kitajima,
Shugoshin collaborates with protein phosphatase 2A to protect cohesin.
2006,
Pubmed
Kong,
Cohesin associates with spindle poles in a mitosis-specific manner and functions in spindle assembly in vertebrate cells.
2009,
Pubmed
Kueng,
Wapl controls the dynamic association of cohesin with chromatin.
2006,
Pubmed
Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed
,
Xenbase
Lénárt,
The small-molecule inhibitor BI 2536 reveals novel insights into mitotic roles of polo-like kinase 1.
2007,
Pubmed
Loncarek,
Centriole reduplication during prolonged interphase requires procentriole maturation governed by Plk1.
2010,
Pubmed
Losada,
Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
McGuinness,
Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.
2005,
Pubmed
Nakamura,
Centrosomal Aki1 and cohesin function in separase-regulated centriole disengagement.
2009,
Pubmed
Riedel,
Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.
2006,
Pubmed
Salic,
Vertebrate shugoshin links sister centromere cohesion and kinetochore microtubule stability in mitosis.
2004,
Pubmed
,
Xenbase
Stemmann,
Dual inhibition of sister chromatid separation at metaphase.
2001,
Pubmed
,
Xenbase
Sumara,
The dissociation of cohesin from chromosomes in prophase is regulated by Polo-like kinase.
2002,
Pubmed
,
Xenbase
Tang,
Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.
2004,
Pubmed
Thein,
Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity.
2007,
Pubmed
Tsou,
Mechanism limiting centrosome duplication to once per cell cycle.
2006,
Pubmed
,
Xenbase
Tsou,
Polo kinase and separase regulate the mitotic licensing of centriole duplication in human cells.
2009,
Pubmed
Uhlmann,
Cleavage of cohesin by the CD clan protease separin triggers anaphase in yeast.
2000,
Pubmed
Uhlmann,
Cohesion between sister chromatids must be established during DNA replication.
1998,
Pubmed
Wang,
sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1.
2008,
Pubmed
Wong,
Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole.
2008,
Pubmed
Wong,
Centrosome number is controlled by a centrosome-intrinsic block to reduplication.
2003,
Pubmed
Zou,
Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.
1999,
Pubmed
,
Xenbase
