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Protein kinase C inhibition of cloned inward rectifier (HRK1/KIR2.3) K+ channels expressed in Xenopus oocytes.
Henry P
,
Pearson WL
,
Nichols CG
.
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1. The effect of protein kinase activators on cloned inward rectifier channels expressed in Xenopus oocytes was examined using a two-electrode voltage clamp. PKA activators caused no change in KIR1.1, KIR2.1, or KIR2.3 current. The PKC activators phorbol 12-myristate 14-acetate (PMA) and phorbol 12, 13-dibutyrate (PDBu) inhibited KIR2.3 currents, but not KIR2.1 or KIR1.1 current. This inhibition was blocked by staurosporine. An inactive phorbol ester, 4 alpha-phorbol 12, 13-didecanoate (4 alpha-PDD), had no effect on KIR2.3. 2. Upon changing solution from 2 to 98 microM K+, KIR2.3 but not KIR1.1 or KIR2.1 currents typically 'ran down' over 5 min to 60-80% of maximum amplitude. Rundown occurred even if PMA was applied before changing to high [K+] solution, indicating that rundown was independent of PKC activity. Rundown was evoked by substituting NMG+ for Na+, showing that it results from low [Na+] and not from high [K+]. 3. These results suggest that KIR2.3, but not KIR1.1 or KIR2.1, is subject to regulation, both by PKC activation and as a consequence of low [Na+]o. The difference in secondary regulation may account for specific responses to PKC stimulation of tissues expressing otherwise nearly identical KIR channels.
Armstrong,
Preconditioning of isolated rabbit cardiomyocytes: induction by metabolic stress and blockade by the adenosine antagonist SPT and calphostin C, a protein kinase C inhibitor.
1994, Pubmed
Armstrong,
Preconditioning of isolated rabbit cardiomyocytes: induction by metabolic stress and blockade by the adenosine antagonist SPT and calphostin C, a protein kinase C inhibitor.
1994,
Pubmed
Billman,
Role of ATP sensitive potassium channel in extracellular potassium accumulation and cardiac arrhythmias during myocardial ischaemia.
1994,
Pubmed
Burckhardt,
Proton transport mechanism in the cell membrane of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Cartaud,
Calcium sequestering activities of reticulum vesicles from Xenopus laevis oocytes.
1984,
Pubmed
,
Xenbase
Doupnik,
The inward rectifier potassium channel family.
1995,
Pubmed
Fakler,
Kir2.1 inward rectifier K+ channels are regulated independently by protein kinases and ATP hydrolysis.
1994,
Pubmed
,
Xenbase
Ficker,
Spermine and spermidine as gating molecules for inward rectifier K+ channels.
1994,
Pubmed
,
Xenbase
Hagiwara,
Potassium current and the effect of cesium on this current during anomalous rectification of the egg cell membrane of a starfish.
1976,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a mouse inward rectifier potassium channel.
1993,
Pubmed
,
Xenbase
Kubo,
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.
1993,
Pubmed
,
Xenbase
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lopatin,
Potassium channel block by cytoplasmic polyamines as the mechanism of intrinsic rectification.
1994,
Pubmed
,
Xenbase
Makhina,
Cloning and expression of a novel human brain inward rectifier potassium channel.
1994,
Pubmed
,
Xenbase
Matsuda,
Ohmic conductance through the inwardly rectifying K channel and blocking by internal Mg2+.
,
Pubmed
Miyake,
Membrane phospholipid turnover in signal transduction; protein kinase C and mechanism of action of tumor promoters.
1983,
Pubmed
Périer,
Primary structure and characterization of a small-conductance inwardly rectifying potassium channel from human hippocampus.
1994,
Pubmed
,
Xenbase
Speechly-Dick,
Protein kinase C. Its role in ischemic preconditioning in the rat.
1994,
Pubmed
Tabcharani,
Regulation of an inwardly rectifying K channel in the T84 epithelial cell line by calcium, nucleotides and kinases.
1994,
Pubmed
Takano,
Protein kinase C-mediated inhibition of an inward rectifier potassium channel by substance P in nucleus basalis neurons.
1995,
Pubmed
Tamaoki,
Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.
1986,
Pubmed
Vandenberg,
Inward rectification of a potassium channel in cardiac ventricular cells depends on internal magnesium ions.
1987,
Pubmed
Wu,
Protein kinase C mediates neurotensin inhibition of inwardly rectifying potassium currents in rat substantia nigra dopaminergic neurons.
1995,
Pubmed
