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XB-ART-8584
Biochem Biophys Res Commun 2001 Aug 17;2862:376-80. doi: 10.1006/bbrc.2001.5392.
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Inactivating properties of recombinant ROMK2 channels expressed in mammalian cells.

Riochet DF , Mohammad-Panah R , Hebert SC , MacGregor GG , Baró I , Guihard G , Escande D .


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Biophysical properties of ROMK2 channel were investigated at physiological temperature, after reexpression of the recombinant ROMK2 protein in a mammalian cell expression system (COS-7). We observed that ROMK2 induced an inwardly rectifying K(+) current whether polyvalent cations were present or not. Above +10 mV, ROMK2-induced current exhibited a voltage- and time-dependent decay, consistent with an inactivation process. Inactivation of ROMK2-induced current was also seen in inside out patch from ROMK2-expressing Xenopus oocyte. In COS-7 cells, inactivation was found to account for most of the inward rectification. Mg(2+) and spermine modulated rectification by accelerating inactivation kinetics independently of membrane potential. These results establish for the first time ROMK2 properties in a mammalian cell expression system.

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