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Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.
Johnson JD
,
Ogden R
,
Johnson P
,
Abelson J
,
Dembeck P
,
Itakura K
.
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The tRNA(3) (Leu) gene from yeast contains an intervening sequence of 32 nucleotides not present in mature tRNA. This sequence is transcribed and subsequently removed during the maturation of the RNA. To probe the involvement of this region of the gene in transcription and processing of the pre-tRNA(3) (Leu), the yeast DNA was cloned in plasmid pBR322 and a 21-base-pair DNA fragment corresponding to the lac operator was inserted into the intervening sequence. Insertion was done at a cleavage site for the restriction endonuclease Hpa I that occurs 19/20 base pairs from the 5' end of the intervening sequence. The parent and modified plasmids were then transcribed in a Xenopus germinal vesicle extract. RNA-fingerprint analysis of the transcription products revealed that both the tRNA(3) (Leu) gene and its modified counterpart were accurately transcribed. Transcription products corresponding to mature tRNA(3) (Leu) and pre-RNA(3) (Leu) with the normal and lac-containing intervening sequence were identified. Precursors extended at their 5' and 3' ends were also present. Both parent and modified genes were transcribed efficiently, and the various products accumulated in similar amounts, indicating that no deleterious effects on transcriptional competence, stability of the transcripts, or processing result from insertion of the 21-base-pair lac operator DNA. Incubation of pre-tRNA molecules that contained intervening sequences but were 5' and 3' mature with a yeast ribosomal wash fraction resulted in excision of the intervening sequence and, in the presence of ATP, ligation of the resulting half-tRNA molecules. The presence of RNA complementary to lac operator DNA neither inhibited the excision and splicing activities nor altered the site of the junction.
Abelson,
RNA processing and the intervening sequence problem.
1979, Pubmed
Abelson,
RNA processing and the intervening sequence problem.
1979,
Pubmed
Bahl,
Chemical and enzymatic synthesis of lactose operator of Escherichia coli and its binding to lactose repressor.
1976,
Pubmed
Beckmann,
Cloning of yeast transfer RNA genes in Escherichia coli.
1977,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
De Robertis,
Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.
1979,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Garber,
Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.
1979,
Pubmed
Goodman,
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
1977,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Hagenbüchle,
The primary transcription product of a silkworm alanine tRNA gene: identification of in vitro sites of initiation, termination and processing.
1979,
Pubmed
,
Xenbase
Knapp,
Splicing of yeast tRNA precursors: structure of the reaction intermediates.
1979,
Pubmed
Knapp,
Transcription and processing of intervening sequences in yeast tRNA genes.
1978,
Pubmed
Kressmann,
A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
1979,
Pubmed
,
Xenbase
Mattoccia,
Separation of RNA transcription and processing activities from X. laevis germinal vesicles.
1979,
Pubmed
,
Xenbase
Ng,
Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
1979,
Pubmed
,
Xenbase
Ogden,
In vitro transcription and processing of a yeast tRNA gene containing an intervening sequence.
1979,
Pubmed
,
Xenbase
Peebles,
Splicing of yeast tRNA precursors: a two-stage reaction.
1979,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Schmidt,
Specific transcription of eukaryotic tRNA genes in Xenopus germinal vesicle extracts.
1978,
Pubmed
,
Xenbase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Struhl,
Functional genetic expression of eukaryotic DNA in Escherichia coli.
1976,
Pubmed
Valenzuela,
Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.
1978,
Pubmed
