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The diversity of thyroid hormone T(3) effects in vivo makes their molecular analysis particularly challenging. Indeed, the current model of the action of T(3) and its receptors on transcription does not reflect this diversity. Here, T(3)-dependent amphibian metamorphosis was exploited to investigate, in an in vivo developmental context, how T(3) directly regulates gene expression. Two, direct positively regulated T(3)-response genes encoding transcription factors were analyzed: thyroid hormone receptor β (TRβ) and TH/bZIP. Reverse transcription-real-time quantitative PCR analysis on Xenopus tropicalis tadpolebrain and tailfin showed differences in expression levels in premetamorphic tadpoles (lower for TH/bZIP than for TRβ) and differences in induction after T(3) treatment (lower for TRβ than for TH/bZIP). To dissect the mechanisms underlying these differences, chromatin immunoprecipitation was used. T(3) differentially induced RNA polymerase II and histone tail acetylation as a function of transcriptional level. Gene-specific patterns of TR binding were found on the different T(3) -responsive elements (higher for TRβ than for TH/bZIP), correlated with gene-specific modifications of H3K4 methylation (higher for TRβ than for TH/bZIP). Moreover, tissue-specific modifications of H3K27 were found (lower in brain than in tailfin). This first in vivo analysis of the association of histone modifications and TR binding/gene activation during vertebrate development for any nuclear receptor indicate that chromatin context of thyroid-responsive elements loci controls the capacity to bind TR through variations in histone H3K4 methylation, and that the histone code, notably H3, contributes to the fine tuning of gene expression that underlies complex physiological T(3) responses.
Akkers,
A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
2009, Pubmed,
Xenbase
Akkers,
A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
2009,
Pubmed
,
Xenbase
Andersen,
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.
2004,
Pubmed
Bannister,
Spatial distribution of di- and tri-methyl lysine 36 of histone H3 at active genes.
2005,
Pubmed
Bernstein,
A bivalent chromatin structure marks key developmental genes in embryonic stem cells.
2006,
Pubmed
Brown,
Amphibian metamorphosis.
2007,
Pubmed
,
Xenbase
Buchholz,
Gene-specific changes in promoter occupancy by thyroid hormone receptor during frog metamorphosis. Implications for developmental gene regulation.
2005,
Pubmed
,
Xenbase
Cao,
Role of histone H3 lysine 27 methylation in Polycomb-group silencing.
2002,
Pubmed
Cloos,
Erasing the methyl mark: histone demethylases at the center of cellular differentiation and disease.
2008,
Pubmed
Coen,
Xenopus Bcl-X(L) selectively protects Rohon-Beard neurons from metamorphic degeneration.
2001,
Pubmed
,
Xenbase
Das,
Identification of direct thyroid hormone response genes reveals the earliest gene regulation programs during frog metamorphosis.
2009,
Pubmed
,
Xenbase
de Luze,
Thyroid hormone-dependent transcriptional regulation of exogenous genes transferred into Xenopus tadpole muscle in vivo.
1993,
Pubmed
,
Xenbase
Forneris,
A highly specific mechanism of histone H3-K4 recognition by histone demethylase LSD1.
2006,
Pubmed
Froidevaux,
The co-chaperone XAP2 is required for activation of hypothalamic thyrotropin-releasing hormone transcription in vivo.
2006,
Pubmed
Furlow,
In vitro and in vivo analysis of the regulation of a transcription factor gene by thyroid hormone during Xenopus laevis metamorphosis.
1999,
Pubmed
,
Xenbase
Garcia-Bassets,
Histone methylation-dependent mechanisms impose ligand dependency for gene activation by nuclear receptors.
2007,
Pubmed
Gillespie,
Retinoid regulated association of transcriptional co-regulators and the polycomb group protein SUZ12 with the retinoic acid response elements of Hoxa1, RARbeta(2), and Cyp26A1 in F9 embryonal carcinoma cells.
2007,
Pubmed
Guenther,
A chromatin landmark and transcription initiation at most promoters in human cells.
2007,
Pubmed
Hartman,
The histone-binding code of nuclear receptor co-repressors matches the substrate specificity of histone deacetylase 3.
2005,
Pubmed
Havis,
Metamorphic T3-response genes have specific co-regulator requirements.
2003,
Pubmed
,
Xenbase
Havis,
Unliganded thyroid hormone receptor is essential for Xenopus laevis eye development.
2006,
Pubmed
,
Xenbase
He,
Nucleosome dynamics define transcriptional enhancers.
2010,
Pubmed
Heintzman,
Histone modifications at human enhancers reflect global cell-type-specific gene expression.
2009,
Pubmed
Huang,
A role for cofactor-cofactor and cofactor-histone interactions in targeting p300, SWI/SNF and Mediator for transcription.
2003,
Pubmed
,
Xenbase
Hublitz,
Mechanisms of transcriptional repression by histone lysine methylation.
2009,
Pubmed
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Mangelsdorf,
The nuclear receptor superfamily: the second decade.
1995,
Pubmed
Martin,
The diverse functions of histone lysine methylation.
2005,
Pubmed
Metzger,
LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
2005,
Pubmed
Okitsu,
DNA methylation dictates histone H3K4 methylation.
2007,
Pubmed
Orford,
Differential H3K4 methylation identifies developmentally poised hematopoietic genes.
2008,
Pubmed
Pinskaya,
H3 lysine 4 di- and tri-methylation deposited by cryptic transcription attenuates promoter activation.
2009,
Pubmed
Ranjan,
Transcriptional repression of Xenopus TR beta gene is mediated by a thyroid hormone response element located near the start site.
1994,
Pubmed
,
Xenbase
Ruthenburg,
Methylation of lysine 4 on histone H3: intricacy of writing and reading a single epigenetic mark.
2007,
Pubmed
Sachs,
An essential role of histone deacetylases in postembryonic organ transformations in Xenopus laevis.
2001,
Pubmed
,
Xenbase
Sachs,
Targeted chromatin binding and histone acetylation in vivo by thyroid hormone receptor during amphibian development.
2000,
Pubmed
,
Xenbase
Schreiber,
Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor.
2001,
Pubmed
,
Xenbase
Shi,
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
2004,
Pubmed
Stock,
Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells.
2007,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Swigut,
H3K27 demethylases, at long last.
2007,
Pubmed
Wang,
Developmental regulation and function of thyroid hormone receptors and 9-cis retinoic acid receptors during Xenopus tropicalis metamorphosis.
2008,
Pubmed
,
Xenbase
Wolffe,
Transcriptional control. Sinful repression.
1997,
Pubmed
Yoon,
Reading and function of a histone code involved in targeting corepressor complexes for repression.
2005,
Pubmed
Yu,
Inferring causal relationships among different histone modifications and gene expression.
2008,
Pubmed
Yu,
A SANT motif in the SMRT corepressor interprets the histone code and promotes histone deacetylation.
2003,
Pubmed
