Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. The xCR1 protein accumulates specifically in the animal cells of Xenopus embryos, but maternal xCR1 mRNA is distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repression of xCR1 mRNA contributes to this spatially restricted accumulation of the xCR1 protein in Xenopus embryos. xCR1 mRNA was associated with polyribosomes in animal cells but not vegetal cells. A 351-nucleotide region of xCR1 mRNA's 3' untranslated region was sufficient to confer a spatially restricted pattern of translation to a luciferase reporter mRNA by repressing translation in vegetal cells. Repression depended upon the mRNA's 5' cap but not its 3' poly(A) tail. Furthermore, the region of xCR1 mRNA sufficient to confer vegetal cell-specific repression contained both Pumilio binding elements (PBEs) and binding sites for the CUG-BP1 protein. The PBEs and the CUG-BP1 sites were necessary but not sufficient for translation repression. Our studies of xCR1 mRNA document the first example of spatially regulated translation in controlling the asymmetric distribution of a maternal determinant in vertebrates.
Cao,
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation.
2002, Pubmed,
Xenbase
Cao,
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation.
2002,
Pubmed
,
Xenbase
Chagnovich,
Poly(A)-independent regulation of maternal hunchback translation in the Drosophila embryo.
2001,
Pubmed
Charlesworth,
Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes.
2004,
Pubmed
,
Xenbase
Chekulaeva,
Bruno acts as a dual repressor of oskar translation, promoting mRNA oligomerization and formation of silencing particles.
2006,
Pubmed
Colegrove-Otero,
RNA-binding proteins in early development.
2005,
Pubmed
de Moor,
Mechanisms of translational control by the 3' UTR in development and differentiation.
2005,
Pubmed
de Moor,
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
1999,
Pubmed
,
Xenbase
Deng,
Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.
2008,
Pubmed
Dorey,
A novel Cripto-related protein reveals an essential role for EGF-CFCs in Nodal signalling in Xenopus embryos.
2006,
Pubmed
,
Xenbase
Fritz,
Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development.
2001,
Pubmed
,
Xenbase
Gallie,
The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency.
1991,
Pubmed
Gavis,
Translational regulation of nanos by RNA localization.
1994,
Pubmed
Goldstrohm,
PUF proteins bind Pop2p to regulate messenger RNAs.
2006,
Pubmed
Goldstrohm,
Multifunctional deadenylase complexes diversify mRNA control.
2008,
Pubmed
Goldstrohm,
PUF protein-mediated deadenylation is catalyzed by Ccr4p.
2007,
Pubmed
Graindorge,
Identification of CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis.
2008,
Pubmed
,
Xenbase
Gu,
A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization.
2004,
Pubmed
Heasman,
Maternal determinants of embryonic cell fate.
2006,
Pubmed
,
Xenbase
Ho,
Transgenic mice expressing CUG-BP1 reproduce splicing mis-regulation observed in myotonic dystrophy.
2005,
Pubmed
Hook,
Two yeast PUF proteins negatively regulate a single mRNA.
2007,
Pubmed
Irish,
The Drosophila posterior-group gene nanos functions by repressing hunchback activity.
1989,
Pubmed
Kim-Ha,
Translational regulation of oskar mRNA by bruno, an ovarian RNA-binding protein, is essential.
1995,
Pubmed
King,
Putting RNAs in the right place at the right time: RNA localization in the frog oocyte.
2005,
Pubmed
,
Xenbase
Kuersten,
The power of the 3' UTR: translational control and development.
2003,
Pubmed
Ladd,
The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.
2001,
Pubmed
Lehmann,
hunchback, a gene required for segmentation of an anterior and posterior region of the Drosophila embryo.
1987,
Pubmed
Marquis,
CUG-BP1/CELF1 requires UGU-rich sequences for high-affinity binding.
2006,
Pubmed
,
Xenbase
Meijer,
Translational control of maskin mRNA by its 3' untranslated region.
2007,
Pubmed
,
Xenbase
Moraes,
CUG-BP binds to RNA substrates and recruits PARN deadenylase.
2006,
Pubmed
,
Xenbase
Mosquera,
A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain.
1993,
Pubmed
,
Xenbase
Murata,
Binding of pumilio to maternal hunchback mRNA is required for posterior patterning in Drosophila embryos.
1995,
Pubmed
Nakahata,
Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation.
2003,
Pubmed
,
Xenbase
Nakahata,
Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein.
2001,
Pubmed
,
Xenbase
Nakamura,
Drosophila cup is an eIF4E binding protein that associates with Bruno and regulates oskar mRNA translation in oogenesis.
2004,
Pubmed
Otero,
A 250-nucleotide UA-rich element in the 3' untranslated region of Xenopus laevis Vg1 mRNA represses translation both in vivo and in vitro.
2001,
Pubmed
,
Xenbase
Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase
Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Philips,
Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy.
1998,
Pubmed
Piqué,
A combinatorial code for CPE-mediated translational control.
2008,
Pubmed
,
Xenbase
Rouhana,
Autoregulation of GLD-2 cytoplasmic poly(A) polymerase.
2007,
Pubmed
,
Xenbase
Schier,
Nodal signaling in vertebrate development.
2003,
Pubmed
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Sonoda,
Recruitment of Nanos to hunchback mRNA by Pumilio.
1999,
Pubmed
Stebbins-Boaz,
Maskin is a CPEB-associated factor that transiently interacts with elF-4E.
1999,
Pubmed
,
Xenbase
Tao,
Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos.
2005,
Pubmed
,
Xenbase
Timchenko,
CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.
1999,
Pubmed
Timchenko,
Age-specific CUGBP1-eIF2 complex increases translation of CCAAT/enhancer-binding protein beta in old liver.
2006,
Pubmed
Vardy,
Regulating translation of maternal messages: multiple repression mechanisms.
2007,
Pubmed
Vasudevan,
Metazoan oocyte and early embryo development program: a progression through translation regulatory cascades.
2006,
Pubmed
,
Xenbase
Vlasova,
Conserved GU-rich elements mediate mRNA decay by binding to CUG-binding protein 1.
2008,
Pubmed
Wharton,
mRNA regulation by Puf domain proteins.
2006,
Pubmed
Wickens,
A PUF family portrait: 3'UTR regulation as a way of life.
2002,
Pubmed
Yabe,
FRL-1, a member of the EGF-CFC family, is essential for neural differentiation in Xenopus early development.
2003,
Pubmed
,
Xenbase
Yost,
The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.
1996,
Pubmed
,
Xenbase
Zhang,
The RNA-binding protein CUGBP1 regulates stability of tumor necrosis factor mRNA in muscle cells: implications for myotonic dystrophy.
2008,
Pubmed
