Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Exploring the phosphoproteome profiles during Xenopus egg activation by calcium stimulation using a fully automated phosphopeptide purification system.
Kanno T
,
Furukawa K
,
Horigome T
.
???displayArticle.abstract???
To explore the phosphoproteome profiles duringXenopusegg activation by Ca(2+)-stimulation, an automated phosphopeptide purification system involving a titania column was improved by introducing 4-step elution with phosphate buffers. The number of detected phosphopeptides in the tryptic digest of aXenopusegg cytosol fraction on mass spectrometry (MS) was increased 1.5-fold and the percentage of multiply phosphorylated peptides increased from 17 to 24% with introduction of the 4-step elution method. Phosphopeptides were purified by the improved method from tryptic digests of cytosol fractions ofXenopuseggs without and with a Ca(2+)-stimulus, and then, analysed by MS. One thousand three hundred and seventy-five and 994 phosphopeptides were reproducibly detected on duplicate MS, respectively. They included 818 and 437 phosphopeptides specific to each digest, respectively. A method involving isobaric tags for relative and absolute quantitation (iTRAQ) was also applied to compare the phosphorylation levels inXenopuseggs without and with a Ca(2+)-stimulus, the ratios for 112 phosphopeptides in tryptic digests of these egg cytosol fractions being obtained. It was suggested from all the results that the phosphorylation sites and levels change duringXenopusegg activation for many known and unknown sites on structural proteins, signalling related proteins, cell cycle-related proteins and others.
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010, Pubmed
Backs,
The gamma isoform of CaM kinase II controls mouse egg activation by regulating cell cycle resumption.
2010,
Pubmed
Bidinosti,
Postnatal deamidation of 4E-BP2 in brain enhances its association with raptor and alters kinetics of excitatory synaptic transmission.
2010,
Pubmed
Cau,
Regulation of Xenopus p21-activated kinase (X-PAK2) by Cdc42 and maturation-promoting factor controls Xenopus oocyte maturation.
2000,
Pubmed
,
Xenbase
Cohen,
Protein phosphatase 4--from obscurity to vital functions.
2005,
Pubmed
Fan,
Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization in mammals.
2004,
Pubmed
Ferguson,
Ser-64 and Ser-111 in PHAS-I are dispensable for insulin-stimulated dissociation from eIF4E.
2003,
Pubmed
Gietzen,
Identification of inhibitory autophosphorylation sites in casein kinase I epsilon.
1999,
Pubmed
Gruhler,
Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.
2005,
Pubmed
Heesom,
Cell cycle-dependent phosphorylation of the translational repressor eIF-4E binding protein-1 (4E-BP1).
2001,
Pubmed
Herbert,
The extracellular signal-regulated kinase pathway regulates the phosphorylation of 4E-BP1 at multiple sites.
2002,
Pubmed
Hörmanseder,
Modulation of cell cycle control during oocyte-to-embryo transitions.
2013,
Pubmed
,
Xenbase
Hornbeck,
PhosphoSitePlus: a comprehensive resource for investigating the structure and function of experimentally determined post-translational modifications in man and mouse.
2012,
Pubmed
Huang,
Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists.
2009,
Pubmed
Iwase,
A fully automated phosphopeptide purification system for large-scale phosphoproteome analysis.
2010,
Pubmed
,
Xenbase
Kanemitsu,
Cdc2-mediated phosphorylation of the gap junction protein, connexin43, during mitosis.
1998,
Pubmed
Kim,
Pathological roles of MAPK signaling pathways in human diseases.
2010,
Pubmed
Krauchunas,
Molecular changes during egg activation.
2013,
Pubmed
Kumano,
Calcium-mediated inactivation of the MAP kinase pathway in sea urchin eggs at fertilization.
2001,
Pubmed
Kyono,
Successive and selective release of phosphorylated peptides captured by hydroxy acid-modified metal oxide chromatography.
2008,
Pubmed
Lampe,
Formation of a distinct connexin43 phosphoisoform in mitotic cells is dependent upon p34cdc2 kinase.
1998,
Pubmed
Liu,
Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism.
2002,
Pubmed
,
Xenbase
Maloverjan,
Dual function of UNC-51-like kinase 3 (Ulk3) in the Sonic hedgehog signaling pathway.
2010,
Pubmed
Maloverjan,
Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway.
2010,
Pubmed
Maudsley,
Gonadotropin-releasing hormone (GnRH) antagonists promote proapoptotic signaling in peripheral reproductive tumor cells by activating a Galphai-coupling state of the type I GnRH receptor.
2004,
Pubmed
McGivern,
Toward defining the phosphoproteome of Xenopus laevis embryos.
2009,
Pubmed
,
Xenbase
Mothe-Satney,
Mammalian target of rapamycin-dependent phosphorylation of PHAS-I in four (S/T)P sites detected by phospho-specific antibodies.
2000,
Pubmed
Nakamura,
Ethylenediaminetetraacetic acid increases identification rate of phosphoproteomics in real biological samples.
2010,
Pubmed
Park,
Discontinuous pH gradient-mediated separation of TiO2-enriched phosphopeptides.
2011,
Pubmed
Pinkse,
Highly robust, automated, and sensitive online TiO2-based phosphoproteomics applied to study endogenous phosphorylation in Drosophila melanogaster.
2008,
Pubmed
Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Sakanaka,
Casein kinase iepsilon in the wnt pathway: regulation of beta-catenin function.
1999,
Pubmed
,
Xenbase
Simpson,
Trafficking, development and hedgehog.
2009,
Pubmed
Sugiyama,
Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications.
2007,
Pubmed
Takano,
The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region.
2002,
Pubmed
,
Xenbase
Tape,
Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC.
2014,
Pubmed
Thingholm,
Analytical strategies for phosphoproteomics.
2009,
Pubmed
Tsai,
Sequential phosphoproteomic enrichment through complementary metal-directed immobilized metal ion affinity chromatography.
2014,
Pubmed
Tunquist,
Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs.
2003,
Pubmed
,
Xenbase
Warn-Cramer,
Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase.
1998,
Pubmed
Warn-Cramer,
Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein.
1996,
Pubmed
