Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1990 Feb 01;102:458-63. doi: 10.1128/mcb.10.2.458-463.1990.
Show Gene links
Show Anatomy links
Effect of microinjection of a low-Mr human placenta protein tyrosine phosphatase on induction of meiotic cell division in Xenopus oocytes.
Tonks NK
,
Cicirelli MF
,
Diltz CD
,
Krebs EG
,
Fischer EH
.
???displayArticle.abstract???
Homogeneous preparations of a protein phosphatase that is specific for phosphotyrosyl residues (protein tyrosine phosphatase [PTPase] 1B) were isolated from human placenta and microinjected into Xenopus oocytes. This resulted in an increase in activity of up to 10-fold over control levels, as measured in homogenates with use of an artificial substrate (reduced carboxamidomethylated and maleylated lysozyme). Microinjected PTPase was stable for at least 18 h. It is distributed within the oocyte in a manner similar to the endogenous activity and is suggestive of an interaction with cellular structures or molecules located predominantly in the animal hemisphere. The phosphatase markedly retarded (by up to 5 h) maturation induced by insulin. This, in conjunction with the demonstration that PTPase 1B abolished insulin stimulation of an S6 peptide (RRLSSLRA) kinase concomitant with a decrease in the phosphorylation of tyrosyl residues in a protein with the same apparent Mr as the beta subunit of the insulin and insulinlike growth factor 1 receptors (M. F. Cicirelli, N. K. Tonks, C. D. Diltz, E. H. Fischer, and E. G. Krebs, submitted for publication), provides further support for an essential role of protein tyrosine phosphorylation in insulin action. Furthermore, maturation was significantly retarded even when the PTPase was injected 2 to 4 h after exposure of the cells to insulin. PTPase 1B also retarded maturation induced by progesterone and maturation-promoting factor, which presumably do not act through the insulin receptor. These data point to a second site of action of the PTPase in the pathway of meiotic cell division, downstream of the insulin receptor and following the appearance of active maturation-promoting factor.
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988, Pubmed,
Xenbase
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase
Charbonneau,
Human placenta protein-tyrosine-phosphatase: amino acid sequence and relationship to a family of receptor-like proteins.
1989,
Pubmed
Charbonneau,
The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.
1988,
Pubmed
Chou,
Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin.
1987,
Pubmed
Cicirelli,
Insulin and progesterone activate a common synthetic ribosomal protein S6 peptide kinase in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Cicirelli,
Internal pH of Xenopus oocytes: a study of the mechanism and role of pH changes during meiotic maturation.
1983,
Pubmed
,
Xenbase
Cicirelli,
Calmodulin synthesis and accumulation during oogenesis and maturation of Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase
Cool,
cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family.
1989,
Pubmed
Debant,
Replacement of insulin receptor tyrosine residues 1162 and 1163 does not alter the mitogenic effect of the hormone.
1988,
Pubmed
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Ebina,
Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin- and antibody-stimulated glucose uptake and receptor kinase activity.
1987,
Pubmed
Ellis,
Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose.
1986,
Pubmed
Forsayeth,
Monoclonal antibodies to the human insulin receptor that activate glucose transport but not insulin receptor kinase activity.
1987,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gherzi,
Reevaluation of the evidence that an antibody to the insulin receptor is insulinmimetic without activating the protein tyrosine kinase activity of the receptor.
1987,
Pubmed
Gurdon,
Methods for nuclear transplantation in amphibia.
1977,
Pubmed
Huchon,
Germinal vesicle breakdown in the Xenopus laevis oocyte: description of a transient microtubular structure.
1981,
Pubmed
,
Xenbase
Hurkman,
Subcellular compartmentalization of maize storage proteins in Xenopus oocytes injected with zein messenger RNAs.
1981,
Pubmed
,
Xenbase
Janicot,
Activation of glucose uptake by insulin and insulin-like growth factor I in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Korn,
Ras p21 as a potential mediator of insulin action in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Lau,
Regional water changes during oocyte meiotic maturation: evidence of ooplasmic segregation.
1984,
Pubmed
Le Marchand-Brustel,
Insulin receptor tyrosine kinase is defective in skeletal muscle of insulin-resistant obese mice.
,
Pubmed
Lohka,
Metaphase protein phosphorylation in Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase
Maller,
A study of the induction of cell division in amphibian oocytes by insulin.
1981,
Pubmed
,
Xenbase
Maller,
Increased phosphorylation of ribosomal protein S6 following microinjection of insulin receptor-kinase into Xenopus oocytes.
,
Pubmed
,
Xenbase
McClain,
A mutant insulin receptor with defective tyrosine kinase displays no biologic activity and does not undergo endocytosis.
1987,
Pubmed
Morgan,
Acute insulin action requires insulin receptor kinase activity: introduction of an inhibitory monoclonal antibody into mammalian cells blocks the rapid effects of insulin.
1987,
Pubmed
Morgan,
Insulin action is blocked by a monoclonal antibody that inhibits the insulin receptor kinase.
1986,
Pubmed
,
Xenbase
Morgan,
Mitosis-specific phosphorylation of p60c-src by p34cdc2-associated protein kinase.
1989,
Pubmed
,
Xenbase
Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed
Okamoto,
Autophosphorylation and kinase activity of insulin receptor in diabetic rats.
1986,
Pubmed
Rosen,
After insulin binds.
1987,
Pubmed
Sadler,
In vivo regulation of cyclic AMP phosphodiesterase in Xenopus oocytes. Stimulation by insulin and insulin-like growth factor 1.
1987,
Pubmed
,
Xenbase
Shenoy,
Purified maturation promoting factor phosphorylates pp60c-src at the sites phosphorylated during fibroblast mitosis.
1989,
Pubmed
,
Xenbase
Simpson,
Insulin receptor phosphorylation may not be a prerequisite for acute insulin action.
1984,
Pubmed
Tonks,
Purification of the major protein-tyrosine-phosphatases of human placenta.
1988,
Pubmed
Tonks,
Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase.
1988,
Pubmed
Tonks,
Characterization of the major protein-tyrosine-phosphatases of human placenta.
1988,
Pubmed
Wu,
Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Yarden,
Growth factor receptor tyrosine kinases.
1988,
Pubmed
Zick,
The role of antireceptor antibodies in stimulating phosphorylation of the insulin receptor.
1984,
Pubmed
