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XB-ART-32709
Proc Natl Acad Sci U S A 1975 Aug 01;728:3065-7.
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Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes.

Marbaix G , Huez G , Burny A , Cleuter Y , Hubert E , Leclercq M , Chantrenne H , Soreq H , Nudel U , Littauer UZ .


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Rabbit globin poly(A)-free and native mRNA preparations were microinjected into Xenopus oocytes. The amount of globin message remaining after incubation of injected oocytes was determined by molecular hybridization with a radioactive complementary DNA probe, synthesized by copying globin mRNA with purified RNA-dependent DNA polymerase (reverse transcriptase) from avian myeloblastosis virus. The results show that 56 hr after injection about 85% of the poly(A)-free mRNA molecules are degraded, while native poly(A)-containing mRNA chains are almost completely preserved during the same period of time.

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References [+] :
Allende, The degradation of ribonucleic acids injected into Xenopus laevis oocytes. 1974, Pubmed, Xenbase