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Expression cloning of TMEM16A as a calcium-activated chloride channel subunit.
Schroeder BC
,
Cheng T
,
Jan YN
,
Jan LY
.
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Calcium-activated chloride channels (CaCCs) are major regulators of sensory transduction, epithelial secretion, and smooth muscle contraction. Other crucial roles of CaCCs include action potential generation in Characean algae and prevention of polyspermia in frog egg membrane. None of the known molecular candidates share properties characteristic of most CaCCs in native cells. Using Axolotl oocytes as an expression system, we have identified TMEM16A as the Xenopus oocyteCaCC. The TMEM16 family of "transmembrane proteins with unknown function" is conserved among eukaryotes, with family members linked to tracheomalacia (mouse TMEM16A), gnathodiaphyseal dysplasia (human TMEM16E), aberrant X segregation (a Drosophila TMEM16 family member), and increased sodium tolerance (yeast TMEM16). Moreover, mouse TMEM16A and TMEM16B yield CaCCs in Axolotl oocytes and mammalian HEK293 cells and recapitulate the broad CaCC expression. The identification of this new family of ion channels may help the development of CaCC modulators for treating diseases including hypertension and cystic fibrosis.
Anderson,
Chloride channels in the apical membrane of normal and cystic fibrosis airway and intestinal epithelia.
1992, Pubmed
Anderson,
Chloride channels in the apical membrane of normal and cystic fibrosis airway and intestinal epithelia.
1992,
Pubmed
Arreola,
Inhibition of Ca(2+)-dependent Cl- channels from secretory epithelial cells by low internal pH.
1995,
Pubmed
Bader,
Voltage-activated and calcium-activated currents studied in solitary rod inner segments from the salamander retina.
1982,
Pubmed
Barish,
A voltage-gated hydrogen ion current in the oocyte membrane of the axolotl, Ambystoma.
1984,
Pubmed
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
Barro-Soria,
Bestrophin 1 and 2 are components of the Ca(2+) activated Cl(-) conductance in mouse airways.
2008,
Pubmed
Bera,
NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate.
2004,
Pubmed
Blaug,
P2 purinoceptors regulate calcium-activated chloride and fluid transport in 31EG4 mammary epithelia.
2003,
Pubmed
Boton,
Two calcium-activated chloride conductances in Xenopus laevis oocytes permeabilized with the ionophore A23187.
1989,
Pubmed
,
Xenbase
Boucher,
Chloride secretory response of cystic fibrosis human airway epithelia. Preservation of calcium but not protein kinase C- and A-dependent mechanisms.
1989,
Pubmed
Callamaras,
Ca(2+)-dependent activation of Cl(-) currents in Xenopus oocytes is modulated by voltage.
2000,
Pubmed
,
Xenbase
Carles,
Head and neck squamous cell carcinoma transcriptome analysis by comprehensive validated differential display.
2006,
Pubmed
Clarke,
Relationship of a non-cystic fibrosis transmembrane conductance regulator-mediated chloride conductance to organ-level disease in Cftr(-/-) mice.
1994,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Decoursey,
Voltage-gated proton channels and other proton transfer pathways.
2003,
Pubmed
Eggermont,
Calcium-activated chloride channels: (un)known, (un)loved?
2004,
Pubmed
Entian,
Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.
1999,
Pubmed
Evans,
Calcium-dependent chloride currents in isolated cells from rat lacrimal glands.
1986,
Pubmed
Frings,
Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species.
2000,
Pubmed
Fromm,
Electrical signals and their physiological significance in plants.
2007,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Hartzell,
Calcium-activated chloride channels.
2005,
Pubmed
Huang,
Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma.
2006,
Pubmed
Jego,
Urodele egg jelly and fertilization.
1986,
Pubmed
Jüschke,
SEC18/NSF-independent, protein-sorting pathway from the yeast cortical ER to the plasma membrane.
2005,
Pubmed
Katoh,
Recent developments in the MAFFT multiple sequence alignment program.
2008,
Pubmed
Khakh,
Dynamic selectivity filters in ion channels.
1999,
Pubmed
Kramer,
The spindle-associated transmembrane protein Axs identifies a membranous structure ensheathing the meiotic spindle.
2003,
Pubmed
Kuruma,
Dynamics of calcium regulation of chloride currents in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Large,
Characteristics and physiological role of the Ca(2+)-activated Cl- conductance in smooth muscle.
1996,
Pubmed
Lennon,
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
1996,
Pubmed
Lupu-Meiri,
Dual regulation by protein kinase C of the muscarinic response in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Melvin,
Regulation of fluid and electrolyte secretion in salivary gland acinar cells.
2005,
Pubmed
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Mizuta,
Molecular characterization of GDD1/TMEM16E, the gene product responsible for autosomal dominant gnathodiaphyseal dysplasia.
2007,
Pubmed
Nilius,
Calcium-activated chloride channels in bovine pulmonary artery endothelial cells.
1997,
Pubmed
Perez-Cornejo,
Permeant anions control gating of calcium-dependent chloride channels.
2004,
Pubmed
Pifferi,
Bestrophin-2 is a candidate calcium-activated chloride channel involved in olfactory transduction.
2006,
Pubmed
Pusch,
Ca(2+)-activated chloride channels go molecular.
2004,
Pubmed
Qu,
Anion permeation in Ca(2+)-activated Cl(-) channels.
2000,
Pubmed
,
Xenbase
Ravin,
Bidirectional replication from an internal ori site of the linear N15 plasmid prophage.
2003,
Pubmed
Rock,
The transmembrane protein TMEM16A is required for normal development of the murine trachea.
2008,
Pubmed
Runft,
Calcium release at fertilization of Xenopus eggs requires type I IP(3) receptors, but not SH2 domain-mediated activation of PLCgamma or G(q)-mediated activation of PLCbeta.
1999,
Pubmed
,
Xenbase
Schaeren-Wiemers,
A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes.
1993,
Pubmed
Sun,
The vitelliform macular dystrophy protein defines a new family of chloride channels.
2002,
Pubmed
Takahashi,
Rat brain serotonin receptors in Xenopus oocytes are coupled by intracellular calcium to endogenous channels.
1987,
Pubmed
,
Xenbase
Takizawa,
Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier.
2000,
Pubmed
Tarran,
Regulation of normal and cystic fibrosis airway surface liquid volume by phasic shear stress.
2006,
Pubmed
Tsien,
Measurement of cytosolic free Ca2+ with quin2.
1989,
Pubmed
Tsutsumi,
The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia (GDD).
2004,
Pubmed
West,
The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFRA mutation status.
2004,
Pubmed
Widdicombe,
Regulation of the depth and composition of airway surface liquid.
2002,
Pubmed
Yang,
TMEM16A confers receptor-activated calcium-dependent chloride conductance.
2008,
Pubmed
,
Xenbase
