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Mol Cell Biol
1999 Jan 01;191:777-87. doi: 10.1128/MCB.19.1.777.
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C-Terminal binding protein is a transcriptional repressor that interacts with a specific class of vertebrate Polycomb proteins.
Sewalt RG
,
Gunster MJ
,
van der Vlag J
,
Satijn DP
,
Otte AP
.
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Polycomb (Pc) is part of a Pc group (PcG) protein complex that is involved in repression of gene activity during Drosophila and vertebrate development. To identify proteins that interact with vertebrate Pc homologs, we performed two-hybrid screens with Xenopus Pc (XPc) and human Pc 2 (HPC2). We find that the C-terminal binding protein (CtBP) interacts with XPc and HPC2, that CtBP and HPC2 coimmunoprecipitate, and that CtBP and HPC2 partially colocalize in large PcG domains in interphase nuclei. CtBP is a protein with unknown function that binds to a conserved 6-amino-acid motif in the C terminus of the adenovirus E1A protein. Also, the Drosophila CtBP homolog interacts, through this conserved amino acid motif, with several segmentation proteins that act as repressors. Similarly, we find that CtBP binds with HPC2 and XPc through the conserved 6-amino-acid motif. Importantly, CtBP does not interact with another vertebrate Pc homolog, M33, which lacks this amino acid motif, indicating specificity among vertebrate Pc homologs. Finally, we show that CtBP is a transcriptional repressor. The results are discussed in terms of a model that brings together PcG-mediated repression and repression systems that require corepressors such as CtBP.
Alkema,
Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.
1997, Pubmed
Alkema,
Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.
1997,
Pubmed
Boyd,
A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and important for the negative modulation of T24-ras mediated transformation, tumorigenesis and metastasis.
1993,
Pubmed
Bunker,
Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.
1994,
Pubmed
Durfee,
The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.
1993,
Pubmed
Franke,
Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster.
1992,
Pubmed
Gecz,
Assignment of a Polycomb-like chromobox gene (CBX2) to human chromosome 17q25.
1995,
Pubmed
Gould,
Functions of mammalian Polycomb group and trithorax group related genes.
1997,
Pubmed
Gunster,
Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic.
1997,
Pubmed
Jones,
Transcriptional modulation by the adenovirus E1A gene.
1995,
Pubmed
Katsanis,
A novel C-terminal binding protein (CTBP2) is closely related to CTBP1, an adenovirus E1A-binding protein, and maps to human chromosome 21q21.3.
1998,
Pubmed
Messmer,
Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster.
1992,
Pubmed
Müller,
Transcriptional silencing by the Polycomb protein in Drosophila embryos.
1995,
Pubmed
Nibu,
Interaction of short-range repressors with Drosophila CtBP in the embryo.
1998,
Pubmed
Pearce,
The mouse has a Polycomb-like chromobox gene.
1992,
Pubmed
Pirrotta,
PcG complexes and chromatin silencing.
1997,
Pubmed
Poortinga,
Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression.
1998,
Pubmed
Rastelli,
Related chromosome binding sites for zeste, suppressors of zeste and Polycomb group proteins in Drosophila and their dependence on Enhancer of zeste function.
1993,
Pubmed
Reijnen,
Polycomb and bmi-1 homologs are expressed in overlapping patterns in Xenopus embryos and are able to interact with each other.
1995,
Pubmed
,
Xenbase
Satijn,
Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
1997,
Pubmed
,
Xenbase
Satijn,
RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
1997,
Pubmed
Schaeper,
Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation.
1995,
Pubmed
Schoorlemmer,
Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain.
1997,
Pubmed
Schumacher,
Murine Polycomb- and trithorax-group genes regulate homeotic pathways and beyond.
1997,
Pubmed
Sewalt,
Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes.
1998,
Pubmed
Sollerbrant,
The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.
1996,
Pubmed
van Lohuizen,
Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes.
1998,
Pubmed
Yoshida,
Transcriptional regulation of the adenovirus E1A gene.
1995,
Pubmed
Zink,
In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster.
1989,
Pubmed
