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XB-ART-4084
Mol Cell 2004 Jan 16;131:137-47. doi: 10.1016/s1097-2765(03)00480-5.
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Cell cycle-regulated recognition of the destruction box of cyclin B by the APC/C in Xenopus egg extracts.

Yamano H , Gannon J , Mahbubani H , Hunt T .


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Substrates for mitotic proteolysis such as cyclin B have a 9 residue destruction motif, the destruction box (D-box). To identify the receptor that specifically binds the D-box, we used affinity chromatography with immobilized D-box matrices. We find that the APC/C from Xenopus egg extracts binds to the D-box of cyclin B, whereas Fizzy (Cdc20) does not. Mutations in the D-box abolished this interaction. We show that this binding is regulated in the cell cycle, such that the APC/C from egg extracts in interphase does not bind to the D-box matrix. Our results suggest that the APC/C forms a stable interaction with the D-box of its substrates in a cell cycle-dependent manner.

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Species referenced: Xenopus
Genes referenced: ccnb1.2

References :
Zachariae, Destruction with a box: substrate recognition by the anaphase-promoting complex. 2004, Pubmed, Xenbase