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microRNAs (miRNAs) are short, non-coding RNAs that regulate gene expression and have prominent roles during early embryo development and organogenesis. We set out to determine the expression pattern of miRNAs in the developmental model system, Xenopus tropicalis. We made probes to predicted primary-miRNA transcripts and performed in situ hybridization. Our data show conserved and novel tissue-specific expression patterns during embryogenesis that suggest functional roles during development.
Fig. 1. Expression patterns of X. tropicalis miRNAs at neurula (A, dorsal view) and early tadpole stages (A′′, lateral view, anterior to the left).
Fig. 2. Xenopus miRNAs exhibit tissue-specific expression patterns. (A), (G), (M), and (S) are lateral views, anterior to the left. All others are 100 μM coronal sections, dorsal at the top. (A) Stage 35 tadpole showing miR-9a-1 expression in sub-ventricular cells of the brain. (G) Stage 24tailbudembryo showing miR-23b expression in the eyeprimordia and the posterior mesenchyme. (M) Stage 35 tadpole showing miR-124 expression in non-proliferative neural tissue. (S) Stage 35 tadpole showing miR-133b staining in head mesoderm, migrating hypaxial muscles, and somites.
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