Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We show that microRNA-427 (miR-427) mediates the rapid deadenylation of maternal mRNAs after the midblastula transition (MBT) of Xenopus laevis embryogenesis. By MBT, the stage when the embryonic cell cycle is remodeled and zygotic transcription of mRNAs is initiated, each embryo has accumulated approximately 10(9) molecules of miR-427 processed from multimeric pri-miR-427 transcripts synthesized after fertilization. We demonstrate that the maternal mRNAs for cyclins A1 and B2 each contain a single miR-427 target sequence, spanning less than 30 nucleotides, that is both necessary and sufficient for deadenylation, and that inactivation of miR-427 leads to stabilization of the mRNAs. Although this deadenylation normally takes place after MBT, exogenous miRNAs produced prematurely in vivo can promote deadenylation prior to MBT, indicating that turnover of the maternal mRNAs is limited by the amount of accumulated miR-427. Injected transcripts comprised solely of the cyclin mRNA 3' untranslated regions or bearing a 5' ApppG cap undergo deadenylation, showing that translation of the targeted RNA is not required. miR-427 is not unique in promoting deadenylation, as an unrelated miRNA, let-7, can substitute for miR-427 if the reporter RNA contains an appropriate let-7 target site. We propose that miR-427, like the orthologous miR-430 of zebrafish, functions to down-regulate expression of maternal mRNAs early in development.
Audic,
Zygotic control of maternal cyclin A1 translation and mRNA stability.
2002, Pubmed,
Xenbase
Audic,
Zygotic control of maternal cyclin A1 translation and mRNA stability.
2002,
Pubmed
,
Xenbase
Audic,
Zygotic regulation of maternal cyclin A1 and B2 mRNAs.
2001,
Pubmed
,
Xenbase
Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase
Bartel,
MicroRNAs: genomics, biogenesis, mechanism, and function.
2004,
Pubmed
Behm-Ansmant,
mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes.
2006,
Pubmed
Bentwich,
Identification of hundreds of conserved and nonconserved human microRNAs.
2005,
Pubmed
Bortolin-Cavaillé,
C19MC microRNAs are processed from introns of large Pol-II, non-protein-coding transcripts.
2009,
Pubmed
Bushati,
Temporal reciprocity of miRNAs and their targets during the maternal-to-zygotic transition in Drosophila.
2008,
Pubmed
Cao,
Evolutionary emergence of microRNAs in human embryonic stem cells.
2008,
Pubmed
Chen,
The developmental miRNA profiles of zebrafish as determined by small RNA cloning.
2005,
Pubmed
Chen,
Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.
2009,
Pubmed
Choi,
Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430.
2007,
Pubmed
Dehlin,
Cap-dependent deadenylation of mRNA.
2000,
Pubmed
,
Xenbase
Didiano,
Molecular architecture of a miRNA-regulated 3' UTR.
2008,
Pubmed
Eulalio,
Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing.
2007,
Pubmed
Eulalio,
The GW182 protein family in animal cells: new insights into domains required for miRNA-mediated gene silencing.
2009,
Pubmed
Eulalio,
Deadenylation is a widespread effect of miRNA regulation.
2009,
Pubmed
Ferjentsik,
Expression patterns of Src-family tyrosine kinases during Xenopus laevis development.
2009,
Pubmed
,
Xenbase
Filipowicz,
Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?
2008,
Pubmed
Galgano,
Comparative analysis of mRNA targets for human PUF-family proteins suggests extensive interaction with the miRNA regulatory system.
2008,
Pubmed
Giraldez,
Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs.
2006,
Pubmed
Giraldez,
MicroRNAs regulate brain morphogenesis in zebrafish.
2005,
Pubmed
Goldstrohm,
PUF proteins bind Pop2p to regulate messenger RNAs.
2006,
Pubmed
Guo,
ElrA and AUF1 differentially bind cyclin B2 mRNA.
2008,
Pubmed
,
Xenbase
Hake,
CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation.
1994,
Pubmed
,
Xenbase
Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase
Heasman,
Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.
2000,
Pubmed
,
Xenbase
Houbaviy,
Embryonic stem cell-specific MicroRNAs.
2003,
Pubmed
Houston,
A Xenopus DAZ-like gene encodes an RNA component of germ plasm and is a functional homologue of Drosophila boule.
1998,
Pubmed
,
Xenbase
Hutvágner,
Sequence-specific inhibition of small RNA function.
2004,
Pubmed
Kappas,
Dissection of the XChk1 signaling pathway in Xenopus laevis embryos.
2000,
Pubmed
,
Xenbase
Keller,
Early embryonic development of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Krieg,
Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs.
1985,
Pubmed
,
Xenbase
Kwak,
Mammalian GLD-2 homologs are poly(A) polymerases.
2004,
Pubmed
,
Xenbase
Lee,
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
1993,
Pubmed
Lund,
Substrate selectivity of exportin 5 and Dicer in the biogenesis of microRNAs.
2006,
Pubmed
,
Xenbase
MacArthur,
DEADSouth is a germ plasm specific DEAD-box RNA helicase in Xenopus related to eIF4A.
2000,
Pubmed
,
Xenbase
Mayr,
Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation.
2007,
Pubmed
Meister,
Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing.
2004,
Pubmed
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Mishima,
Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430.
2006,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Nilsen,
Mechanisms of microRNA-mediated gene regulation in animal cells.
2007,
Pubmed
Nolde,
The Caenorhabditis elegans pumilio homolog, puf-9, is required for the 3'UTR-mediated repression of the let-7 microRNA target gene, hbl-1.
2007,
Pubmed
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Pasquinelli,
Reverse 5' caps in RNAs made in vitro by phage RNA polymerases.
1995,
Pubmed
,
Xenbase
Piqué,
A combinatorial code for CPE-mediated translational control.
2008,
Pubmed
,
Xenbase
Price,
Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies.
1995,
Pubmed
Rosa,
The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection.
2009,
Pubmed
,
Xenbase
Rouhana,
Autoregulation of GLD-2 cytoplasmic poly(A) polymerase.
2007,
Pubmed
,
Xenbase
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Suh,
Human embryonic stem cells express a unique set of microRNAs.
2004,
Pubmed
Tang,
Xenopus microRNA genes are predominantly located within introns and are differentially expressed in adult frog tissues via post-transcriptional regulation.
2008,
Pubmed
,
Xenbase
Tian,
Post-transcriptional regulation of Xwnt-8 expression is required for normal myogenesis during vertebrate embryonic development.
1999,
Pubmed
,
Xenbase
Uto,
Two structural variants of Nek2 kinase, termed Nek2A and Nek2B, are differentially expressed in Xenopus tissues and development.
1999,
Pubmed
,
Xenbase
Vella,
Architecture of a validated microRNA::target interaction.
2004,
Pubmed
Watanabe,
Stage-specific expression of microRNAs during Xenopus development.
2005,
Pubmed
,
Xenbase
Weigel,
A tiny helper lightens the maternal load.
2006,
Pubmed
Wickens,
A PUF family portrait: 3'UTR regulation as a way of life.
2002,
Pubmed
Wu,
MicroRNAs direct rapid deadenylation of mRNA.
2006,
Pubmed
Wu,
Structural basis of m(7)GpppG binding to poly(A)-specific ribonuclease.
2009,
Pubmed
Wu,
Importance of translation and nonnucleolytic ago proteins for on-target RNA interference.
2008,
Pubmed
Yang,
Beta-catenin/Tcf-regulated transcription prior to the midblastula transition.
2002,
Pubmed
,
Xenbase
Zamore,
The Pumilio protein binds RNA through a conserved domain that defines a new class of RNA-binding proteins.
1997,
Pubmed
Zhang,
Single processing center models for human Dicer and bacterial RNase III.
2004,
Pubmed
