Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Single channel properties of human alpha3 AChRs: impact of beta2, beta4 and alpha5 subunits.
Nelson ME
,
Lindstrom J
.
???displayArticle.abstract???
1. We performed single channel analysis on human alpha3 acetylcholine receptors (AChRs) in Xenopus oocytes and native AChRs from the human neuroblastoma cell line IMR-32. alpha3 AChRs exhibit channel properties that reflect subunit composition. 2. alpha3beta2 AChR open times were 0.71 +/- 0.14 and 3.5 +/- 0.4 ms with a predominant conductance of 26 pS. alpha3beta4 AChRs had open times of 1.4 +/- 0.2 and 6.5 +/- 0.8 ms and a predominant conductance of 31 pS. Burst times were 0.82 +/- 0.12 and 5.3 +/- 0.7 ms for alpha3beta2 and 1.7 +/- 0.1 and 16 +/- 1 ms for alpha3beta4. Desensitization was faster for AChRs with the beta2 subunit than for those with the beta4 subunit. 3. One open time for alpha3alpha5beta2 AChRs (5.5 +/- 0.3 ms) was different from those of alpha3beta2 AChRs. For alpha3alpha5beta4 AChRs, an additional conductance, open time and burst time (36 pS, 22 +/- 3 ms and 43 +/- 4 ms, respectively) were different from those for alpha3beta4 AChRs. 4. alpha3 AChRs were inhibited by hexamethonium or mecamylamine. The rate constants for block of alpha3beta4 by hexamethonium and of alpha3beta2 by mecamylamine were 1.2 x 107 and 4.6 x 107 M-1 s-1, respectively. 5. AChRs from IMR-32 cells had a predominant conductance of 32 pS and open times of 1.5 +/- 0.3 and 9.6 +/- 1.2 ms. These properties were most similar to those of alpha3beta4 AChRs expressed in oocytes. Antibodies revealed that 5 +/- 2 % of IMR-32 alpha3 AChRs contained alpha5 subunits and 6 +/- 2 % contained beta2 subunits. IMR-32 alpha3 AChRs are primarily alpha3beta4 AChRs.
Alkondon,
Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. II. The rundown and inward rectification of agonist-elicited whole-cell currents and identification of receptor subunits by in situ hybridization.
1994, Pubmed
Alkondon,
Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. II. The rundown and inward rectification of agonist-elicited whole-cell currents and identification of receptor subunits by in situ hybridization.
1994,
Pubmed
Anand,
Nucleotide sequence of the human nicotinic acetylcholine receptor beta 2 subunit gene.
1990,
Pubmed
Cachelin,
Beta subunits determine the time course of desensitization in rat alpha 3 neuronal nicotinic acetylcholine receptors.
1991,
Pubmed
,
Xenbase
Chavez-Noriega,
Pharmacological characterization of recombinant human neuronal nicotinic acetylcholine receptors h alpha 2 beta 2, h alpha 2 beta 4, h alpha 3 beta 2, h alpha 3 beta 4, h alpha 4 beta 2, h alpha 4 beta 4 and h alpha 7 expressed in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Chini,
Neuronal-type alpha-bungarotoxin receptors and the alpha 5-nicotinic receptor subunit gene are expressed in neuronal and nonneuronal human cell lines.
1992,
Pubmed
Conroy,
Neurons can maintain multiple classes of nicotinic acetylcholine receptors distinguished by different subunit compositions.
1995,
Pubmed
Conroy,
The alpha 5 gene product assembles with multiple acetylcholine receptor subunits to form distinctive receptor subtypes in brain.
1992,
Pubmed
Covernton,
Comparison of neuronal nicotinic receptors in rat sympathetic neurones with subunit pairs expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Elliott,
Comparative structure of human neuronal alpha 2-alpha 7 and beta 2-beta 4 nicotinic acetylcholine receptor subunits and functional expression of the alpha 2, alpha 3, alpha 4, alpha 7, beta 2, and beta 4 subunits.
1996,
Pubmed
,
Xenbase
Fenwick,
A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine.
1982,
Pubmed
Figl,
Voltage-jump relaxation kinetics for wild-type and chimeric beta subunits of neuronal nicotinic receptors.
1996,
Pubmed
,
Xenbase
Forsayeth,
Formation of oligomers containing the beta3 and beta4 subunits of the rat nicotinic receptor.
1997,
Pubmed
Fucile,
The neuronal alpha6 subunit forms functional heteromeric acetylcholine receptors in human transfected cells.
1998,
Pubmed
Gerzanich,
"Orphan" alpha6 nicotinic AChR subunit can form a functional heteromeric acetylcholine receptor.
1997,
Pubmed
,
Xenbase
Gerzanich,
alpha 5 Subunit alters desensitization, pharmacology, Ca++ permeability and Ca++ modulation of human neuronal alpha 3 nicotinic receptors.
1998,
Pubmed
,
Xenbase
Gotti,
Native nicotinic acetylcholine receptors in human Imr32 neuroblastoma cells: functional, immunological and pharmacological properties.
1995,
Pubmed
Gotti,
Human neuronal nicotinic receptors.
1997,
Pubmed
Groot Kormelink,
Cloning and sequence of full-length cDNAs encoding the human neuronal nicotinic acetylcholine receptor (nAChR) subunits beta3 and beta4 and expression of seven nAChR subunits in the human neuroblastoma cell line SH-SY5Y and/or IMR-32.
1997,
Pubmed
Groot-Kormelink,
A reporter mutation approach shows incorporation of the "orphan" subunit beta3 into a functional nicotinic receptor.
1998,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Huganir,
Regulation of neurotransmitter receptor desensitization by protein phosphorylation.
1990,
Pubmed
Jaramillo,
Kinetic differences between embryonic- and adult-type acetylcholine receptors in rat myotubes.
1988,
Pubmed
Karlin,
Toward a structural basis for the function of nicotinic acetylcholine receptors and their cousins.
1995,
Pubmed
Kuryatov,
Mutation causing autosomal dominant nocturnal frontal lobe epilepsy alters Ca2+ permeability, conductance, and gating of human alpha4beta2 nicotinic acetylcholine receptors.
1997,
Pubmed
,
Xenbase
Lewis,
The ion channel properties of a rat recombinant neuronal nicotinic receptor are dependent on the host cell type.
1997,
Pubmed
,
Xenbase
Lindstrom,
Neuronal nicotinic acetylcholine receptors.
1996,
Pubmed
Luetje,
Neurotoxins distinguish between different neuronal nicotinic acetylcholine receptor subunit combinations.
1990,
Pubmed
,
Xenbase
Luetje,
Both alpha- and beta-subunits contribute to the agonist sensitivity of neuronal nicotinic acetylcholine receptors.
1991,
Pubmed
,
Xenbase
Lukas,
Expression of ganglia-type nicotinic acetylcholine receptors and nicotinic ligand binding sites by cells of the IMR-32 human neuroblastoma clonal line.
1993,
Pubmed
Magleby,
Burst kinetics of single calcium-activated potassium channels in cultured rat muscle.
1983,
Pubmed
Mandelzys,
The developmental increase in ACh current densities on rat sympathetic neurons correlates with changes in nicotinic ACh receptor alpha-subunit gene expression and occurs independent of innervation.
1994,
Pubmed
Margiotta,
Changes in the number, function, and regulation of nicotinic acetylcholine receptors during neuronal development.
1989,
Pubmed
McGehee,
Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons.
1995,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Moss,
Enhanced ACh sensitivity is accompanied by changes in ACh receptor channel properties and segregation of ACh receptor subtypes on sympathetic neurons during innervation in vivo.
1993,
Pubmed
Neher,
Correction for liquid junction potentials in patch clamp experiments.
1992,
Pubmed
Papke,
Single-channel currents of rat neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Papke,
The kinetic properties of neuronal nicotinic receptors: genetic basis of functional diversity.
1993,
Pubmed
Papke,
Partial agonist properties of cytisine on neuronal nicotinic receptors containing the beta 2 subunit.
1994,
Pubmed
,
Xenbase
Papke,
The role of the beta 4-subunit in determining the kinetic properties of rat neuronal nicotinic acetylcholine alpha 3-receptors.
1991,
Pubmed
,
Xenbase
Ramirez-Latorre,
Functional contributions of alpha5 subunit to neuronal acetylcholine receptor channels.
1996,
Pubmed
,
Xenbase
Sargent,
The diversity of neuronal nicotinic acetylcholine receptors.
1993,
Pubmed
Sivilotti,
Recombinant nicotinic receptors, expressed in Xenopus oocytes, do not resemble native rat sympathetic ganglion receptors in single-channel behaviour.
1997,
Pubmed
,
Xenbase
Sivilotti,
Acetylcholine receptors: too many channels, too few functions.
1995,
Pubmed
Tumilowicz,
Definition of a continuous human cell line derived from neuroblastoma.
1970,
Pubmed
Ullian,
Rapid synaptic transmission in the avian ciliary ganglion is mediated by two distinct classes of nicotinic receptors.
1997,
Pubmed
Unwin,
Acetylcholine receptor channel imaged in the open state.
1995,
Pubmed
Vernallis,
Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes.
1993,
Pubmed
Wang,
Chronic nicotine treatment up-regulates human alpha3 beta2 but not alpha3 beta4 acetylcholine receptors stably transfected in human embryonic kidney cells.
1998,
Pubmed
,
Xenbase
Wang,
Assembly of human neuronal nicotinic receptor alpha5 subunits with alpha3, beta2, and beta4 subunits.
1996,
Pubmed
,
Xenbase
Whiting,
Characterization of bovine and human neuronal nicotinic acetylcholine receptors using monoclonal antibodies.
1988,
Pubmed
Wilson Horch,
Synaptic and extrasynaptic distribution of two distinct populations of nicotinic acetylcholine receptor clusters in the frog cardiac ganglion.
1996,
Pubmed
