Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1992 Jun 01;116:2025-32. doi: 10.1002/j.1460-2075.1992.tb05259.x.
Show Gene links
Show Anatomy links
Agonist pharmacology of neonatal and adult glycine receptor alpha subunits: identification of amino acid residues involved in taurine activation.
Schmieden V
,
Kuhse J
,
Betz H
.
???displayArticle.abstract???
The inhibitory glycine receptor (GlyR) is a pentameric chloride channel protein which mediates postsynaptic inhibition in the mammalian central nervous system. In spinal cord, different GlyR isoforms originate from the sequential expression of developmentally regulated variants of the ligand binding alpha subunit. Here, neonatal alpha 2 and adult alpha 1 subunits are shown to generate GlyRs with distinct agonist activation profiles upon heterologous expression in Xenopus oocytes. Whereas alpha 1 receptors are efficiently gated by beta-alanine and taurine, alpha 2 GlyRs show only a low relative response to these agonists, which also display a reduced sensitivity to inhibition by the glycinergic antagonist strychnine. Construction of an alpha 2/alpha 1 subunit chimera and site-directed mutagenesis of the extracellular region of the alpha 1 sequence identified amino acid positions 111 and 212 as important determinants of taurine activation. Our results indicate the existence of distinct subsites for agonists on alpha 1 and alpha 2 GlyRs and suggest that the ligand binding pocket of these receptor proteins is formed from discontinuous domains of their extracellular region.
Abramson,
An analog of lophotoxin reacts covalently with Tyr190 in the alpha-subunit of the nicotinic acetylcholine receptor.
1989, Pubmed
Abramson,
An analog of lophotoxin reacts covalently with Tyr190 in the alpha-subunit of the nicotinic acetylcholine receptor.
1989,
Pubmed
Akagi,
Discrimination of heterogenous mRNAs encoding strychnine-sensitive glycine receptors in Xenopus oocytes by antisense oligonucleotides.
1989,
Pubmed
,
Xenbase
Akagi,
Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord.
1988,
Pubmed
,
Xenbase
Akagi,
Cloning of a glycine receptor subtype expressed in rat brain and spinal cord during a specific period of neuronal development.
1991,
Pubmed
,
Xenbase
Becker,
Glycine receptor heterogeneity in rat spinal cord during postnatal development.
1988,
Pubmed
Betz,
Ligand-gated ion channels in the brain: the amino acid receptor superfamily.
1990,
Pubmed
Changeux,
The acetylcholine receptor: an "allosteric" membrane protein.
,
Pubmed
Cutler,
Regional changes in amino acid content in developing rat brain.
1974,
Pubmed
Dennis,
Amino acids of the Torpedo marmorata acetylcholine receptor alpha subunit labeled by a photoaffinity ligand for the acetylcholine binding site.
1988,
Pubmed
Galzi,
Identification of a novel amino acid alpha-tyrosine 93 within the cholinergic ligands-binding sites of the acetylcholine receptor by photoaffinity labeling. Additional evidence for a three-loop model of the cholinergic ligands-binding sites.
1990,
Pubmed
Graham,
Purification and characterization of the glycine receptor of pig spinal cord.
1985,
Pubmed
Graham,
Photoaffinity-labelling of the glycine receptor of rat spinal cord.
1983,
Pubmed
Graham,
UV light-induced cross-linking of strychnine to the glycine receptor of rat spinal cord membranes.
1981,
Pubmed
Grenningloh,
Cloning and expression of the 58 kd beta subunit of the inhibitory glycine receptor.
1990,
Pubmed
,
Xenbase
Grenningloh,
The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors.
,
Pubmed
Grenningloh,
Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes.
1990,
Pubmed
,
Xenbase
Hoch,
Primary cultures of mouse spinal cord express the neonatal isoform of the inhibitory glycine receptor.
1989,
Pubmed
Kao,
Acetylcholine receptor binding site contains a disulfide cross-link between adjacent half-cystinyl residues.
1986,
Pubmed
Kuhse,
Alternative splicing generates two isoforms of the alpha 2 subunit of the inhibitory glycine receptor.
1991,
Pubmed
,
Xenbase
Kuhse,
Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor.
1990,
Pubmed
,
Xenbase
Kuhse,
A single amino acid exchange alters the pharmacology of neonatal rat glycine receptor subunit.
1990,
Pubmed
,
Xenbase
Langosch,
Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer.
1988,
Pubmed
Langosch,
The inhibitory glycine receptor: a ligand-gated chloride channel of the central nervous system.
1990,
Pubmed
Malosio,
Widespread expression of glycine receptor subunit mRNAs in the adult and developing rat brain.
1991,
Pubmed
Malosio,
Alternative splicing generates two variants of the alpha 1 subunit of the inhibitory glycine receptor.
1991,
Pubmed
,
Xenbase
Pfeiffer,
Purification by affinity chromatography of the glycine receptor of rat spinal cord.
1982,
Pubmed
Pfeiffer,
Monoclonal antibodies and peptide mapping reveal structural similarities between the subunits of the glycine receptor of rat spinal cord.
1984,
Pubmed
Pritchett,
gamma-Aminobutyric acid type A receptor point mutation increases the affinity of compounds for the benzodiazepine site.
1991,
Pubmed
Ruiz-Gómez,
Localization of the strychnine binding site on the 48-kilodalton subunit of the glycine receptor.
1990,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmieden,
Functional expression in Xenopus oocytes of the strychnine binding 48 kd subunit of the glycine receptor.
1989,
Pubmed
,
Xenbase
Schröder,
Mapping of antigenic epitopes on the alpha 1 subunit of the inhibitory glycine receptor.
1991,
Pubmed
Sontheimer,
Functional chloride channels by mammalian cell expression of rat glycine receptor subunit.
1989,
Pubmed
Tokutomi,
What confers specificity on glycine for its receptor site?
1989,
Pubmed
Trautmann,
A comparative study of the activation of the cholinergic receptor by various agonists.
1983,
Pubmed
