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Proc Natl Acad Sci U S A
2014 Jun 03;11122:8281-6. doi: 10.1073/pnas.1324060111.
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Acid-sensing ion channel (ASIC) 1a/2a heteromers have a flexible 2:1/1:2 stoichiometry.
Bartoi T
,
Augustinowski K
,
Polleichtner G
,
Gründer S
,
Ulbrich MH
.
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Acid-sensing ion channels (ASICs) are widely expressed proton-gated Na(+) channels playing a role in tissue acidosis and pain. A trimeric composition of ASICs has been suggested by crystallization. Upon coexpression of ASIC1a and ASIC2a in Xenopus oocytes, we observed the formation of heteromers and their coexistence with homomers by electrophysiology, but could not determine whether heteromeric complexes have a fixed subunit stoichiometry or whether certain stoichiometries are preferred over others. We therefore imaged ASICs labeled with green and red fluorescent proteins on a single-molecule level, counted bleaching steps from GFP and colocalized them with red tandem tetrameric mCherry for many individual complexes. Combinatorial analysis suggests a model of random mixing of ASIC1a and ASIC2a subunits to yield both 2:1 and 1:2 ASIC1a:ASIC2a heteromers together with ASIC1a and ASIC2a homomers.
Anantharam,
Determination of epithelial Na+ channel subunit stoichiometry from single-channel conductances.
2007, Pubmed,
Xenbase
Anantharam,
Determination of epithelial Na+ channel subunit stoichiometry from single-channel conductances.
2007,
Pubmed
,
Xenbase
Askwith,
Acid-sensing ion channel 2 (ASIC2) modulates ASIC1 H+-activated currents in hippocampal neurons.
2004,
Pubmed
Baconguis,
Structural plasticity and dynamic selectivity of acid-sensing ion channel-spider toxin complexes.
2012,
Pubmed
Baron,
Acid sensing ion channels in dorsal spinal cord neurons.
2008,
Pubmed
Baron,
ASIC-like, proton-activated currents in rat hippocampal neurons.
2002,
Pubmed
Barrera,
The subunit arrangement and assembly of ionotropic receptors.
2008,
Pubmed
Bassilana,
The acid-sensitive ionic channel subunit ASIC and the mammalian degenerin MDEG form a heteromultimeric H+-gated Na+ channel with novel properties.
1997,
Pubmed
,
Xenbase
Carnally,
Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging.
2008,
Pubmed
Chen,
Interaction of acid-sensing ion channel (ASIC) 1 with the tarantula toxin psalmotoxin 1 is state dependent.
2006,
Pubmed
,
Xenbase
Dawson,
Structure of the acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1.
2012,
Pubmed
Dijkink,
The epithelial sodium channel (ENaC) is intracellularly located as a tetramer.
2002,
Pubmed
,
Xenbase
Diochot,
Black mamba venom peptides target acid-sensing ion channels to abolish pain.
2012,
Pubmed
,
Xenbase
Eskandari,
Number of subunits comprising the epithelial sodium channel.
1999,
Pubmed
,
Xenbase
Firsov,
The heterotetrameric architecture of the epithelial sodium channel (ENaC).
1998,
Pubmed
,
Xenbase
Friese,
Acid-sensing ion channel-1 contributes to axonal degeneration in autoimmune inflammation of the central nervous system.
2007,
Pubmed
Gao,
Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel.
2007,
Pubmed
Gonzales,
Pore architecture and ion sites in acid-sensing ion channels and P2X receptors.
2009,
Pubmed
Gründer,
Structure, function, and pharmacology of acid-sensing ion channels (ASICs): focus on ASIC1a.
2010,
Pubmed
Gründer,
Toxin binding reveals two open state structures for one acid-sensing ion channel.
2012,
Pubmed
Hines,
Inferring subunit stoichiometry from single molecule photobleaching.
2013,
Pubmed
Jain,
Probing cellular protein complexes using single-molecule pull-down.
2011,
Pubmed
Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed
Kosari,
Subunit stoichiometry of the epithelial sodium channel.
1998,
Pubmed
,
Xenbase
Kumar,
Structure and assembly mechanism for heteromeric kainate receptors.
2011,
Pubmed
,
Xenbase
Li,
Developmental change in the electrophysiological and pharmacological properties of acid-sensing ion channels in CNS neurons.
2010,
Pubmed
Lingueglia,
A modulatory subunit of acid sensing ion channels in brain and dorsal root ganglion cells.
1997,
Pubmed
Sherwood,
Heteromeric acid-sensing ion channels (ASICs) composed of ASIC2b and ASIC1a display novel channel properties and contribute to acidosis-induced neuronal death.
2011,
Pubmed
,
Xenbase
Snyder,
Electrophysiological and biochemical evidence that DEG/ENaC cation channels are composed of nine subunits.
1998,
Pubmed
,
Xenbase
Staruschenko,
Fluorescence resonance energy transfer analysis of subunit stoichiometry of the epithelial Na+ channel.
2004,
Pubmed
Sutherland,
Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons.
2001,
Pubmed
Ulbrich,
Subunit counting in membrane-bound proteins.
2007,
Pubmed
,
Xenbase
Vukicevic,
Modulatory effects of acid-sensing ion channels on action potential generation in hippocampal neurons.
2004,
Pubmed
Wemmie,
The acid-activated ion channel ASIC contributes to synaptic plasticity, learning, and memory.
2002,
Pubmed
Wu,
Characterization of acid-sensing ion channels in dorsal horn neurons of rat spinal cord.
2004,
Pubmed
Wu,
Acid-sensing ion channel-1a is not required for normal hippocampal LTP and spatial memory.
2013,
Pubmed
Xiong,
Neuroprotection in ischemia: blocking calcium-permeable acid-sensing ion channels.
2004,
Pubmed
Zha,
ASIC2 subunits target acid-sensing ion channels to the synapse via an association with PSD-95.
2009,
Pubmed
