Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression and functional characterization of four aquaporin water channels from the European eel (Anguilla anguilla).
MacIver B
,
Cutler CP
,
Yin J
,
Hill MG
,
Zeidel ML
,
Hill WG
.
???displayArticle.abstract???
The European eel is a euryhaline teleost which has been shown to differentially up- and downregulate aquaporin (AQP) water channels in response to changes in environmental salinity. We have characterized the transport properties of four aquaporins localized to osmoregulatory organs - gill, esophagus, intestine and kidney. By sequence comparison these four AQP orthologs resemble human AQP1 (eel AQP1), AQP3 (eel AQP3) and AQP10 (AQPe). The fourth member is a duplicate form of AQP1 (AQP1dup) thought to arise from a duplication of the teleost genome. Using heterologous expression in Xenopus oocytes we demonstrate that all four eel orthologs transport water and are mercury inhibitable. Eel AQP3 and AQPe also transport urea and glycerol, making them aquaglyceroporins. Eel AQP3 is dramatically inhibited by extracellular acidity (91% and 69% inhibition of water and glycerol transport respectively at pH 6.5) consistent with channel gating by protons. Maximal water flux of eel AQP3 occurred around pH 8.2 - close to the physiological pH of plasma in the eel. Exposure of AQP-expressing oocytes to heavy metals revealed that eel AQP3 is highly sensitive to extracellular nickel and zinc (88.3% and 86.3% inhibition, respectively) but less sensitive to copper (56.4% inhibition). Surprisingly, copper had a stimulatory effect on eel AQP1 (153.7% activity of control). Copper, nickel and zinc did not affect AQP1dup or AQPe. We establish that all four eel AQP orthologs have similar transport profiles to their human counterparts, with eel AQP3 exhibiting some differences in its sensitivity to metals. This is the first investigation of the transport properties and inhibitor sensitivity of salinity-regulated aquaporins from a euryhaline species. Our results indicate a need to further investigate the deleterious effects of metal pollutants on AQP-containing epithelial cells of the gill and gastrointestinal tract at environmentally appropriate concentrations.
Ando,
Water metabolism in the eel acclimated to sea water: from mouth to intestine.
2003, Pubmed
Ando,
Water metabolism in the eel acclimated to sea water: from mouth to intestine.
2003,
Pubmed
Beyenbach,
Kidneys sans glomeruli.
2004,
Pubmed
Blanchard,
Copper toxicity across salinities from freshwater to seawater in the euryhaline fish Fundulus heteroclitus: is copper an ionoregulatory toxicant in high salinities?
2006,
Pubmed
Bucking,
The alkaline tide and ammonia excretion after voluntary feeding in freshwater rainbow trout.
2008,
Pubmed
Calamita,
The Escherichia coli aquaporin-Z water channel.
2000,
Pubmed
Cooper,
Post-prandial alkaline tide in freshwater rainbow trout: effects of meal anticipation on recovery from acid-base and ion regulatory disturbances.
2008,
Pubmed
Cutler,
Cortisol regulates eel (Anguilla anguilla) aquaporin 3 (AQP3) mRNA expression levels in gill.
2007,
Pubmed
Cutler,
The role of aquaporin 3 in teleost fish.
2007,
Pubmed
Cutler,
Branchial expression of an aquaporin 3 (AQP-3) homologue is downregulated in the European eel Anguilla anguilla following seawater acclimation.
2002,
Pubmed
Dean,
Glucocorticoid receptor upregulation during seawater adaptation in a euryhaline teleost, the tilapia (Oreochromis mossambicus).
2003,
Pubmed
Evans,
The fish gill: site of action and model for toxic effects of environmental pollutants.
1987,
Pubmed
Evans,
The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste.
2005,
Pubmed
Froger,
Functional characterization of a microbial aquaglyceroporin.
2001,
Pubmed
,
Xenbase
Grosell,
Effects of prolonged copper exposure in the marine gulf toadfish (Opsanus beta) II: copper accumulation, drinking rate and Na+/K+ -ATPase activity in osmoregulatory tissues.
2004,
Pubmed
Grosell,
Physiology is pivotal for interactions between salinity and acute copper toxicity to fish and invertebrates.
2007,
Pubmed
Grosell,
Sodium turnover rate determines sensitivity to acute copper and silver exposure in freshwater animals.
2002,
Pubmed
Hogstrand,
Covariation in regulation of affinity for branchial zinc and calcium uptake in freshwater rainbow trout.
1998,
Pubmed
Hwang,
New insights into fish ion regulation and mitochondrion-rich cells.
2007,
Pubmed
Kieffer,
EFFECTS OF ENVIRONMENTAL TEMPERATURE ON THE METABOLIC AND ACID-BASE RESPONSES OF RAINBOW TROUT TO EXHAUSTIVE EXERCISE.
1994,
Pubmed
King,
From structure to disease: the evolving tale of aquaporin biology.
2004,
Pubmed
Kozono,
Functional expression and characterization of an archaeal aquaporin. AqpM from methanothermobacter marburgensis.
2003,
Pubmed
,
Xenbase
Kuwahara,
Mercury-sensitive residues and pore site in AQP3 water channel.
1997,
Pubmed
,
Xenbase
Lazowski,
Evidence for the presence of a Hg-inhibitable water-permeability pathway and aquaporin 1 in A5 salivary epithelial cells.
1995,
Pubmed
Lee,
Structural basis for conductance by the archaeal aquaporin AqpM at 1.68 A.
2005,
Pubmed
Lignot,
Immunolocalisation of aquaporin 3 in the gill and the gastrointestinal tract of the European eel Anguilla anguilla (L.).
2002,
Pubmed
Martinez,
Regulation of expression of two aquaporin homologs in the intestine of the European eel: effects of seawater acclimation and cortisol treatment.
2005,
Pubmed
Martinez,
Cloning and expression of three aquaporin homologues from the European eel (Anguilla anguilla): effects of seawater acclimation and cortisol treatment on renal expression.
2005,
Pubmed
Masoni,
Urea, inulin and para-amino-hippuric acid (PAH) excretion by the gills of the eel, Anguilla anguilla L.
1974,
Pubmed
Maurel,
Plant aquaporins: membrane channels with multiple integrated functions.
2008,
Pubmed
Mulders,
Importance of the mercury-sensitive cysteine on function and routing of AQP1 and AQP2 in oocytes.
1997,
Pubmed
,
Xenbase
Niyogi,
Interaction between dietary calcium supplementation and chronic waterborne zinc exposure in juvenile rainbow trout, Oncorhynchus mykiss.
2006,
Pubmed
Pandey,
Effects of exposure to multiple trace metals on biochemical, histological and ultrastructural features of gills of a freshwater fish, Channa punctata Bloch.
2008,
Pubmed
Pane,
Renal function in the freshwater rainbow trout (Oncorhynchus mykiss) following acute and prolonged exposure to waterborne nickel.
2005,
Pubmed
Pane,
Hydromineral balance in the marine gulf toadfish (Opsanus beta) exposed to waterborne or infused nickel.
2006,
Pubmed
Richards,
Exercise and recovery metabolism in the Pacific spiny dogfish (Squalus acanthias).
2003,
Pubmed
Savage,
Structural basis of aquaporin inhibition by mercury.
2007,
Pubmed
Terreros,
Nickel inhibition of the osmotic-sensitive ionic cellular channels.
1988,
Pubmed
Verkman,
More than just water channels: unexpected cellular roles of aquaporins.
2005,
Pubmed
Yang,
Water and glycerol permeabilities of aquaporins 1-5 and MIP determined quantitatively by expression of epitope-tagged constructs in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Zardoya,
A phylogenetic framework for the aquaporin family in eukaryotes.
2001,
Pubmed
Zelenina,
Copper inhibits the water and glycerol permeability of aquaporin-3.
2004,
Pubmed
Zelenina,
Nickel and extracellular acidification inhibit the water permeability of human aquaporin-3 in lung epithelial cells.
2003,
Pubmed
Zeuthen,
Transport of water and glycerol in aquaporin 3 is gated by H(+).
1999,
Pubmed
,
Xenbase
Zhang,
Gastrointestinal uptake of cadmium and zinc by a marine teleost Acanthopagrus schlegeli.
2007,
Pubmed
