Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Am J Physiol Regul Integr Comp Physiol
2010 Mar 01;2983:R642-60. doi: 10.1152/ajpregu.00729.2009.
Show Gene links
Show Anatomy links
A SLC4-like anion exchanger from renal tubules of the mosquito (Aedes aegypti): evidence for a novel role of stellate cells in diuretic fluid secretion.
Piermarini PM
,
Grogan LF
,
Lau K
,
Wang L
,
Beyenbach KW
.
???displayArticle.abstract???
Transepithelial fluid secretion across the renal (Malpighian) tubule epithelium of the mosquito (Aedes aegypti) is energized by the vacuolar-type (V-type) H(+)-ATPase and not the Na(+)-K(+)-ATPase. Located at the apical membrane of principal cells, the V-type H(+)-ATPase translocates protons from the cytoplasm to the tubule lumen. Secreted protons are likely to derive from metabolic H(2)CO(3), which raises questions about the handling of HCO(3)(-) by principal cells. Accordingly, we tested the hypothesis that a Cl/HCO(3) anion exchanger (AE) related to the solute-linked carrier 4 (SLC4) superfamily mediates the extrusion of HCO(3)(-) across the basal membrane of principal cells. We began by cloning from Aedes Malpighian tubules a full-length cDNA encoding an SLC4-like AE, termed AeAE. When expressed heterologously in Xenopus oocytes, AeAE is both N- and O-glycosylated and mediates Na(+)-independent intracellular pH changes that are sensitive to extracellular Cl(-) concentration and to DIDS. In Aedes Malpighian tubules, AeAE is expressed as two distinct forms: one is O-glycosylated, and the other is N-glycosylated. Significantly, AeAE immunoreactivity localizes to the basal regions of stellate cells but not principal cells. Concentrations of DIDS that inhibit AeAE activity in Xenopus oocytes have no effects on the unstimulated rates of fluid secretion mediated by Malpighian tubules as measured by the Ramsay assay. However, in Malpighian tubules stimulated with kinin or calcitonin-like diuretic peptides, DIDS reduces the diuretic rates of fluid secretion to basal levels. In conclusion, Aedes Malpighian tubules express AeAE in the basal region of stellate cells, where this transporter may participate in producing diuretic rates of transepithelial fluid secretion.
Alper,
Molecular physiology and genetics of Na+-independent SLC4 anion exchangers.
2009, Pubmed
Alper,
Molecular physiology and genetics of Na+-independent SLC4 anion exchangers.
2009,
Pubmed
Bertram,
Carbonic anhydrase supports electrolyte transport in Drosophila Malpighian tubules. Evidence by X-ray microanalysis of cryosections.
1997,
Pubmed
Beyenbach,
Regulation of tight junction permeability with switch-like speed.
2003,
Pubmed
Beyenbach,
Membrane conductances of principal cells in Malpighian tubules of Aedes aegypti.
2002,
Pubmed
Beyenbach,
Energizing epithelial transport with the vacuolar H(+)-ATPase.
2001,
Pubmed
Beyenbach,
Signaling to the apical membrane and to the paracellular pathway: changes in the cytosolic proteome of Aedes Malpighian tubules.
2009,
Pubmed
Beyenbach,
Transport mechanisms of diuresis in Malpighian tubules of insects.
2003,
Pubmed
Boron,
Modular structure of sodium-coupled bicarbonate transporters.
2009,
Pubmed
Brown,
Mitochondria-rich, proton-secreting epithelial cells.
1996,
Pubmed
Brown,
Role of V-ATPase-rich cells in acidification of the male reproductive tract.
1997,
Pubmed
Casey,
Enzymatic deglycosylation of human Band 3, the anion transport protein of the erythrocyte membrane. Effect on protein structure and transport properties.
1992,
Pubmed
Chen,
Use of a new polyclonal antibody to study the distribution and glycosylation of the sodium-coupled bicarbonate transporter NCBE in rodent brain.
2008,
Pubmed
,
Xenbase
Cheung,
Topology of transmembrane segments 1-4 in the human chloride/bicarbonate anion exchanger 1 (AE1) by scanning N-glycosylation mutagenesis.
2005,
Pubmed
Choi,
Role of glycosylation in the renal electrogenic Na+-HCO3- cotransporter (NBCe1).
2003,
Pubmed
,
Xenbase
Coast,
Mosquito natriuretic peptide identified as a calcitonin-like diuretic hormone in Anopheles gambiae (Giles).
2005,
Pubmed
Combet,
NPS@: network protein sequence analysis.
2000,
Pubmed
Ehrenfeld,
The key role of the H+ V-ATPase in acid-base balance and Na+ transport processes in frog skin.
1997,
Pubmed
Espiritu,
A central role for Pyk2-Src interaction in coupling diverse stimuli to increased epithelial NBC activity.
2002,
Pubmed
Fiévet,
Expression of band 3 anion exchanger induces chloride current and taurine transport: structure-function analysis.
1995,
Pubmed
,
Xenbase
Grichtchenko,
Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.
2001,
Pubmed
,
Xenbase
Gross,
Phosphorylation of Ser(982) in the sodium bicarbonate cotransporter kNBC1 shifts the HCO(3)(-) : Na(+) stoichiometry from 3 : 1 to 2 : 1 in murine proximal tubule cells.
2001,
Pubmed
Groves,
Role of N-glycosylation in the expression of human band 3-mediated anion transport.
1994,
Pubmed
,
Xenbase
Gutiérrez,
A model for fluid secretion in Rhodnius upper Malpighian tubules (UMT).
2004,
Pubmed
Hegarty,
Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules.
1991,
Pubmed
Helman,
Interpretation and use of electrical equivalent circuits in studies of epithelial tissues.
1982,
Pubmed
Hübner,
Amino acid sequence of band-3 protein from rainbow trout erythrocytes derived from cDNA.
1992,
Pubmed
Humphreys,
Functional characterization and regulation by pH of murine AE2 anion exchanger expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Ianowski,
Electrochemical gradients for Na+, K+, Cl- and H+ across the apical membrane in Malpighian (renal) tubule cells of Rhodnius prolixus.
2006,
Pubmed
Jay,
Glycosylation site of band 3, the human erythrocyte anion-exchange protein.
1986,
Pubmed
Jiang,
pHi and serum regulate AE2-mediated Cl-/HCO3- exchange in CHOP cells of defined transient transfection status.
1994,
Pubmed
Kietz,
pH-dependence of inhibition by H2DIDS of mouse erythroid band 3-mediated Cl- transport in Xenopus oocytes. The effect of oligonucleotide-directed replacement of Lys-558 by an Asn residue.
1991,
Pubmed
,
Xenbase
Kopito,
Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger.
1989,
Pubmed
Kurita,
Identification of intestinal bicarbonate transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish.
2008,
Pubmed
,
Xenbase
Kurschat,
Alkaline-shifted pHo sensitivity of AE2c1-mediated anion exchange reveals novel regulatory determinants in the AE2 N-terminal cytoplasmic domain.
2006,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Landolt-Marticorena,
Asparagine-linked oligosaccharides are localized to single extracytosolic segments in multi-span membrane glycoproteins.
1994,
Pubmed
Larkin,
Clustal W and Clustal X version 2.0.
2007,
Pubmed
Lee,
Functional differences among nonerythroid anion exchangers expressed in a transfected human cell line.
1991,
Pubmed
Lindsey,
Functional expression and subcellular localization of an anion exchanger cloned from choroid plexus.
1990,
Pubmed
Lu,
Reversible and irreversible interactions of DIDS with the human electrogenic Na/HCO3 cotransporter NBCe1-A: role of lysines in the KKMIK motif of TM5.
2007,
Pubmed
,
Xenbase
Medina,
Molecular cloning and characterization of the human AE2 anion exchanger (SLC4A2) gene.
1997,
Pubmed
Nazeer,
Changes in protein profiles during course of experimental glomerulonephritis.
2009,
Pubmed
Nene,
Genome sequence of Aedes aegypti, a major arbovirus vector.
2007,
Pubmed
O'Connor,
Chloride channels in apical membrane patches of stellate cells of Malpighian tubules of Aedes aegypti.
2001,
Pubmed
O'Donnell,
Hormonally controlled chloride movement across Drosophila tubules is via ion channels in stellate cells.
1998,
Pubmed
Okubo,
Red blood cell band 3. Lysine 539 and lysine 851 react with the same H2DIDS (4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid) molecule.
1994,
Pubmed
Pannabecker,
Regulation of epithelial shunt conductance by the peptide leucokinin.
1993,
Pubmed
Parker,
Cloning and characterization of novel human SLC4A8 gene products encoding Na+-driven Cl-/HCO3(-) exchanger variants NDCBE-A, -C, and -D.
2008,
Pubmed
,
Xenbase
Parker,
The disruption of the third extracellular loop of the red cell anion exchanger AE1 does not affect electroneutral Cl-/HCO3- exchange activity.
2004,
Pubmed
,
Xenbase
Parker,
A conductive pathway generated from fragments of the human red cell anion exchanger AE1.
2007,
Pubmed
,
Xenbase
Patrick,
P-type Na+/K+-ATPase and V-type H+-ATPase expression patterns in the osmoregulatory organs of larval and adult mosquito Aedes aegypti.
2006,
Pubmed
Petzel,
Na(+)/H(+) exchange in mosquito Malpighian tubules.
2000,
Pubmed
Petzel,
Intracellular and luminal pH measurements of Malpighian tubules of the mosquito Aedes aegypti: the effects of cAMP.
1999,
Pubmed
Piermarini,
Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe.
2007,
Pubmed
,
Xenbase
Piermarini,
NHE8 is an intracellular cation/H+ exchanger in renal tubules of the yellow fever mosquito Aedes aegypti.
2009,
Pubmed
,
Xenbase
Pullikuth,
Molecular characterization of sodium/proton exchanger 3 (NHE3) from the yellow fever vector, Aedes aegypti.
2006,
Pubmed
Rath,
Detergent binding explains anomalous SDS-PAGE migration of membrane proteins.
2009,
Pubmed
Romero,
Cloning and characterization of a Na+-driven anion exchanger (NDAE1). A new bicarbonate transporter.
2000,
Pubmed
,
Xenbase
Romero,
The SLC4 family of HCO 3 - transporters.
2004,
Pubmed
Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase
Schmitt,
Immunolocalization of the electrogenic Na+-HCO-3 cotransporter in mammalian and amphibian kidney.
1999,
Pubmed
,
Xenbase
Sciortino,
Localization of endogenous and recombinant Na(+)-driven anion exchanger protein NDAE1 from Drosophila melanogaster.
2001,
Pubmed
,
Xenbase
Sciortino,
Cation and voltage dependence of rat kidney electrogenic Na(+)-HCO(-)(3) cotransporter, rkNBC, expressed in oocytes.
1999,
Pubmed
,
Xenbase
Shmukler,
Zebrafish slc4a2/ae2 anion exchanger: cDNA cloning, mapping, functional characterization, and localization.
2005,
Pubmed
,
Xenbase
Smith,
Cloning and characterization of AgCA9, a novel alpha-carbonic anhydrase from Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) larvae.
2007,
Pubmed
Sterling,
Transport activity of AE3 chloride/bicarbonate anion-exchange proteins and their regulation by intracellular pH.
1999,
Pubmed
Stewart,
Putative re-entrant loop 1 of AE2 transmembrane domain has a major role in acute regulation of anion exchange by pH.
2009,
Pubmed
,
Xenbase
Stewart,
Acute regulation of mouse AE2 anion exchanger requires isoform-specific amino acid residues from most of the transmembrane domain.
2007,
Pubmed
,
Xenbase
Stewart,
Acute pH-dependent regulation of AE2-mediated anion exchange involves discrete local surfaces of the NH2-terminal cytoplasmic domain.
2004,
Pubmed
,
Xenbase
Stewart,
Regulation of AE2-mediated Cl- transport by intracellular or by extracellular pH requires highly conserved amino acid residues of the AE2 NH2-terminal cytoplasmic domain.
2002,
Pubmed
,
Xenbase
Stewart,
Role of nonconserved charged residues of the AE2 transmembrane domain in regulation of anion exchange by pH.
2007,
Pubmed
,
Xenbase
Stewart,
Transmembrane domain histidines contribute to regulation of AE2-mediated anion exchange by pH.
2007,
Pubmed
,
Xenbase
Stewart,
Regulation of AE2 anion exchanger by intracellular pH: critical regions of the NH(2)-terminal cytoplasmic domain.
2001,
Pubmed
,
Xenbase
Supanchart,
Ion channels and transporters in osteoclasts.
2008,
Pubmed
Tamura,
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.
2007,
Pubmed
Tanner,
The complete amino acid sequence of the human erythrocyte membrane anion-transport protein deduced from the cDNA sequence.
1988,
Pubmed
Terhzaz,
Differential gel electrophoresis and transgenic mitochondrial calcium reporters demonstrate spatiotemporal filtering in calcium control of mitochondria.
2006,
Pubmed
Toye,
Defective kidney anion-exchanger 1 (AE1, Band 3) trafficking in dominant distal renal tubular acidosis (dRTA).
2005,
Pubmed
Trudeau,
HERG, a human inward rectifier in the voltage-gated potassium channel family.
1995,
Pubmed
Velez,
Characterization of renin-angiotensin system enzyme activities in cultured mouse podocytes.
2007,
Pubmed
Virkki,
Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2).
2002,
Pubmed
,
Xenbase
Virkki,
Cloning of a Na+-driven Cl/HCO3 exchanger from squid giant fiber lobe.
2003,
Pubmed
,
Xenbase
Wagner,
Renal vacuolar H+-ATPase.
2004,
Pubmed
Weng,
The V-type H(+)-ATPase in Malpighian tubules of Aedes aegypti: localization and activity.
2003,
Pubmed
Weng,
Gap junctions in Malpighian tubules of Aedes aegypti.
2008,
Pubmed
Xu,
Effects of inhibitors and specific ion-free salines on segmental fluid secretion by the Malpighian tubules of the black field cricket Teleogryllus oceanicus.
1999,
Pubmed
Yu,
Effects of leucokinin-VIII on Aedes Malpighian tubule segments lacking stellate cells.
2004,
Pubmed
Yu,
Leucokinin activates Ca(2+)-dependent signal pathway in principal cells of Aedes aegypti Malpighian tubules.
2002,
Pubmed
Zhu,
The substrate anion selectivity filter in the human erythrocyte Cl-/HCO3- exchange protein, AE1.
2004,
Pubmed
Zhu,
Novel topology in C-terminal region of the human plasma membrane anion exchanger, AE1.
2003,
Pubmed
Zolotarev,
HCO3(-)-dependent conformational change in gastric parietal cell AE2, a glycoprotein naturally lacking sialic acid.
1996,
Pubmed
Zolotarev,
Proteolytic cleavage sites of native AE2 anion exchanger in gastric mucosal membranes.
1996,
Pubmed
Zubrzak,
Beta-amino acid analogs of an insect neuropeptide feature potent bioactivity and resistance to peptidase hydrolysis.
2007,
Pubmed
