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EMBO J
1989 Oct 01;810:3059-69. doi: 10.1002/j.1460-2075.1989.tb08457.x.
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A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
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A cell-free extract prepared from activated Xenopus eggs by high-speed centrifugation displays one spontaneous cycle of activation and inactivation of histone H1 kinase and MPF activity that is largely attributable to Xenopus p32cdc2. The timing of the oscillation closely follows that observed in intact eggs, is associated with large changes in endogenous protein phosphorylation and depends entirely on post-translational events. The extract can be fractionated into soluble and particulate material, both of which components are required for the oscillatory behaviour. Kinase activation does not require Mg+ ATP, but its rapid inactivation, which coincides with the destruction of cyclin, is inhibited both by EDTA and the protein kinase inhibitor 6-dimethylaminopurine. This suggests that protein phosphorylation is required for cyclin destruction and kinase inactivation.
Ajiro,
Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycle.
1981, Pubmed
Ajiro,
Phosphorylation states of different histone 1 subtypes and their relationship to chromatin functions during the HeLa S-3 cell cycle.
1981,
Pubmed
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase
Bradbury,
Phosphorylation of very-lysine-rich histone in Physarum polycephalum. Correlation with chromosome condensation.
1973,
Pubmed
Brizuela,
p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
1987,
Pubmed
Capoly,
Changes in the activity of the maturation-promoting factor during meiotic maturation and following activation of amphibian and starfish oocytes: their correlations with protein phosphorylation.
1986,
Pubmed
,
Xenbase
Dabauvalle,
Role of nuclear material in the early cell cycle of Xenopus embryos.
1988,
Pubmed
,
Xenbase
Dorée,
Activity of the maturation-promoting factor and the extent of protein phosphorylation oscillate simultaneously during meiotic maturation of starfish oocytes.
1983,
Pubmed
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Forbes,
Small nuclear RNA transcription and ribonucleoprotein assembly in early Xenopus development.
1983,
Pubmed
,
Xenbase
Ford,
Maturation promoting factor and cell cycle regulation.
1985,
Pubmed
,
Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Guerrier,
Hormonal control of meiosis in starfish: stimulation of protein phosphorylation induced by 1-methyladenine.
1977,
Pubmed
Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Hindley,
Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases.
1984,
Pubmed
Hindley,
Sucl+ encodes a predicted 13-kilodalton protein that is essential for cell viability and is directly involved in the division cycle of Schizosaccharomyces pombe.
1987,
Pubmed
Karsenti,
Phosphorylation changes associated with the early cell cycle in Xenopus eggs.
1987,
Pubmed
,
Xenbase
Karsenti,
Interconversion of metaphase and interphase microtubule arrays, as studied by the injection of centrosomes and nuclei into Xenopus eggs.
1984,
Pubmed
,
Xenbase
Labbe,
Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Xenbase
Labbé,
An M-phase-specific protein kinase of Xenopus oocytes: partial purification and possible mechanism of its periodic activation.
1988,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lohka,
Effects of Ca2+ ions on the formation of metaphase chromosomes and sperm pronuclei in cell-free preparations from unactivated Rana pipiens eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase
Maller,
Changes in protein phosphorylation accompanying maturation of Xenopus laevis oocytes.
1977,
Pubmed
,
Xenbase
Meijer,
Stimulation of protein phosphorylation during fertilization-induced maturation of Urechis caupo oocytes.
1982,
Pubmed
Miake-Lye,
Induction of early mitotic events in a cell-free system.
1985,
Pubmed
,
Xenbase
Miake-Lye,
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Neant,
6-Dimethylaminopurine blocks starfish oocyte maturation by inhibiting a relevant protein kinase activity.
1988,
Pubmed
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Peaucellier,
Rise and fall of protein phosphorylation during meiotic maturation in oocytes of Sabellaria alveolata (polychaete annelid).
1984,
Pubmed
Picard,
Role of protein synthesis and proteases in production and inactivation of maturation-promoting activity during meiotic maturation of starfish oocytes.
1985,
Pubmed
Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
,
Xenbase
Rebhun,
Cleavage inhibition in marine eggs by puromycin and 6-dimethylaminopurine.
1973,
Pubmed
Reed,
Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.
1985,
Pubmed
Russell,
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.
1987,
Pubmed
Simanis,
The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation.
1986,
Pubmed
Standart,
Cyclin synthesis, modification and destruction during meiotic maturation of the starfish oocyte.
1987,
Pubmed
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Wagenaar,
The timing of synthesis of proteins required for mitosis in the cell cycle of the sea urchin embryo.
1983,
Pubmed
