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XB-ART-44475
Vet Immunol Immunopathol 2012 Jan 15;1451-2:493-8. doi: 10.1016/j.vetimm.2011.10.019.
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Oral immunization of the African clawed frog (Xenopus laevis) upregulates the mucosal immunoglobulin IgX.

Du CC , Mashoof SM , Criscitiello MF .


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The frog Xenopus laevis is a model species for developmental biology but is also of significant interest to comparative immunologists. Amphibians are the oldest group of organisms in which both the B lymphocytes of some species undergo immunoglobulin (Ig) class switch recombination and also have a dedicated mucosal Ig isotype. The purpose of this study was to test the hypothesis that frog IgX would be produced in response to oral immunization. In order to facilitate studies of humoral, and especially mucosal immunity, in this model species, we developed a gavage technique for oral immunization. The result of this oral administration of antigen to frogs was assayed by the induction of the mucosal antibody isotype, IgX, in plasma by enzyme linked immunosorbant assay (ELISA), and a significant IgX upregulation was detected compared to frogs receiving systemic immunization into the coelom. These data are consistent with the view that IgX is the functional analog of mammalian IgA and mandate further studies of the relationship between IgX and IgA. Additionally, the gavage technique should be adaptable for functional studies of gut-associated immunology in other small aquatic vertebrates.

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Species referenced: Xenopus laevis
Genes referenced: XB5798883

References [+] :
Atcha, Alternative method of oral dosing for rats. 2010, Pubmed