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Bioassay for components regulating eukaryotic gene expression: a chromosomal factor involved in the generation of histone mRNA 3' termini.
Stunnenberg HG
,
Birnstiel ML
.
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We have adapted the oocyte injection procedure for the detection of regulatory components involved in the transcription of a eukaryotic mRNA gene. Injection of the histone gene repeat h22 DNA of Psammechinus miliaris into the Xenopus oocyte nucleus results in correct initiation of the histone mRNAs, but readthrough by RNA polymerase occurs at the 3' end of the H3 histone gene (Hentschel, C. C., Probst, E. & Birnstiel, M. L. (1980) Nature (London) 288, 100-102). Coinjection into the oocyte of a chromosomal salt wash fraction derived from sea urchin embryos results in the generation of authentic 3' termini of the histone H3 mRNA. We have partially purified the protein component by column chromatography and density gradient centrifugation. The regulatory factor binds to heparin columns and, hence, has the properties anticipated of an RNA- or DNA-binding protein. The sedimentation coefficient of the active component was determined to be about 12 S, suggesting a molecular weight of 200,000-250,000.
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977, Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
Chatterjee,
Proteins in nucleocytoplasmic interactions. V. Intranuclear localization of proteins in Amoeba proteus.
1971,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Diaz,
Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus.
1981,
Pubmed
Grosschedl,
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
1980,
Pubmed
,
Xenbase
Grosschedl,
Delimitation of far upstream sequences required for maximal in vitro transcription of an H2A histone gene.
1982,
Pubmed
Hentschel,
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Kedes,
Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
1971,
Pubmed
Kressmann,
Transcription of xenopus tDNAmet1 and sea urchin histone DNA injected into the Xenopus oocyte nucleus.
1978,
Pubmed
,
Xenbase
Levy,
Sea urchin nuclei use RNA polymerase II to transcribe discrete histone RNAs larger than messengers.
1978,
Pubmed
Platt,
Termination of transcription and its regulation in the tryptophan operon of E. coli.
1981,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Rosenberg,
Regulatory sequences involved in the promotion and termination of RNA transcription.
1979,
Pubmed
Schaffner,
Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.
1976,
Pubmed
Spelsberg,
Deoxyribonucleoproteins and the tissue-specific restriction of the deoxyribonucleic acid in chromatin.
1970,
Pubmed
Stunnenberg,
RNA polymerase from the fungus, Aspergillus nidulans. Large-scale purification of DNA-dependent RNA polymerase I (or A).
1979,
Pubmed
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Weinberg,
Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence.
1972,
Pubmed
