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Channels (Austin)
2011 Jan 01;51:43-55. doi: 10.4161/chan.5.1.13963.
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K(V)4.3 N-terminal deletion mutant Δ2-39: effects on inactivation and recovery characteristics in both the absence and presence of KChIP2b.
Hovind LJ
,
Skerritt MR
,
Campbell DL
.
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Gating transitions in the K(V)4.3 N-terminal deletion mutant Δ2-39 were characterized in the absence and presence of KChIP2b. We particularly focused on gating characteristics of macroscopic (open state) versus closed state inactivation (CSI) and recovery. In the absence of KChIP2b Δ2-39 did not significantly alter the steady-state activation "a(4)" relationship or general CSI characteristics, but it did slow the kinetics of deactivation, macroscopic inactivation, and macroscopic recovery. Recovery kinetics (for both WT K(V)4.3 and Δ2-39) were complicated and displayed sigmoidicity, a process which was enhanced by Δ2-39. Deletion of the proximal N-terminal domain therefore appeared to specifically slow mechanisms involved in regulating gating transitions occurring after the channel open state(s) had been reached. In the presence of KChIP2b Δ2-39 recovery kinetics (from both macroscopic and CSI) were accelerated, with an apparent reduction in initial sigmoidicity. Hyperpolarizing shifts in both "a(4)" and isochronal inactivation "i" were also produced. KChIP2b-mediated remodeling of K(V)4.3 gating transitions was therefore not obligatorily dependent upon an intact N-terminus. To account for these effects we propose that KChIP2 regulatory domains exist in K(V)4.3 a subunit regions outside of the proximal N-terminal. In addition to regulating macroscopic inactivation, we also propose that the K(V)4.3 N-terminus may act as a novel regulator of deactivation-recovery coupling.
Aldrich,
Fifty years of inactivation.
2001, Pubmed
Aldrich,
Fifty years of inactivation.
2001,
Pubmed
Amadi,
Regulation of Kv4.3 closed state inactivation and recovery by extracellular potassium and intracellular KChIP2b.
2007,
Pubmed
,
Xenbase
Amarillo,
Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons.
2008,
Pubmed
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Bähring,
Kinetic analysis of open- and closed-state inactivation transitions in human Kv4.2 A-type potassium channels.
2001,
Pubmed
Bähring,
Conserved Kv4 N-terminal domain critical for effects of Kv channel-interacting protein 2.2 on channel expression and gating.
2001,
Pubmed
Barghaan,
Dynamic coupling of voltage sensor and gate involved in closed-state inactivation of kv4.2 channels.
2009,
Pubmed
,
Xenbase
Barghaan,
Role of N-terminal domain and accessory subunits in controlling deactivation-inactivation coupling of Kv4.2 channels.
2008,
Pubmed
Baukrowitz,
Modulation of K+ current by frequency and external [K+]: a tale of two inactivation mechanisms.
1995,
Pubmed
Bett,
Modification of K+ channel-drug interactions by ancillary subunits.
2008,
Pubmed
Bezanilla,
The voltage sensor in voltage-dependent ion channels.
2000,
Pubmed
Bezanilla,
Voltage-gated ion channels.
2005,
Pubmed
Birnbaum,
Structure and function of Kv4-family transient potassium channels.
2004,
Pubmed
Callsen,
Contribution of N- and C-terminal Kv4.2 channel domains to KChIP interaction [corrected].
2005,
Pubmed
Choi,
Tetraethylammonium blockade distinguishes two inactivation mechanisms in voltage-activated K+ channels.
1991,
Pubmed
Comer,
Cloning and characterization of an Ito-like potassium channel from ferret ventricle.
1994,
Pubmed
,
Xenbase
Covarrubias,
The neuronal Kv4 channel complex.
2008,
Pubmed
Decher,
Novel KChIP2 isoforms increase functional diversity of transient outward potassium currents.
2004,
Pubmed
,
Xenbase
Demo,
The inactivation gate of the Shaker K+ channel behaves like an open-channel blocker.
1991,
Pubmed
Deschênes,
Modulation of Kv4.3 current by accessory subunits.
2002,
Pubmed
Dougherty,
Gating charge immobilization in Kv4.2 channels: the basis of closed-state inactivation.
2008,
Pubmed
Dougherty,
A dipeptidyl aminopeptidase-like protein remodels gating charge dynamics in Kv4.2 channels.
2006,
Pubmed
,
Xenbase
Gebauer,
N-type inactivation features of Kv4.2 channel gating.
2004,
Pubmed
Han,
C-terminal domain of Kv4.2 and associated KChIP2 interactions regulate functional expression and gating of Kv4.2.
2006,
Pubmed
Hasdemir,
Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway.
2005,
Pubmed
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Hoshi,
Two types of inactivation in Shaker K+ channels: effects of alterations in the carboxy-terminal region.
1991,
Pubmed
,
Xenbase
Hu,
The kv4.2 potassium channel subunit is required for pain plasticity.
2006,
Pubmed
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Jerng,
Molecular physiology and modulation of somatodendritic A-type potassium channels.
2004,
Pubmed
Jerng,
Modulation of Kv4.2 channel expression and gating by dipeptidyl peptidase 10 (DPP10).
2004,
Pubmed
,
Xenbase
Kim,
Regulation of dendritic excitability by activity-dependent trafficking of the A-type K+ channel subunit Kv4.2 in hippocampal neurons.
2007,
Pubmed
Kim,
Three-dimensional structure of I(to); Kv4.2-KChIP2 ion channels by electron microscopy at 21 Angstrom resolution.
2004,
Pubmed
Kim,
Kv4 potassium channel subunits control action potential repolarization and frequency-dependent broadening in rat hippocampal CA1 pyramidal neurones.
2005,
Pubmed
Kim,
Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons.
2008,
Pubmed
Korn,
Potassium channels.
2005,
Pubmed
Kuo,
A defect in the Kv channel-interacting protein 2 (KChIP2) gene leads to a complete loss of I(to) and confers susceptibility to ventricular tachycardia.
2001,
Pubmed
Levy,
The membrane protein MiRP3 regulates Kv4.2 channels in a KChIP-dependent manner.
2010,
Pubmed
López-Barneo,
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.
1993,
Pubmed
,
Xenbase
Lundby,
KCNE3 is an inhibitory subunit of the Kv4.3 potassium channel.
2006,
Pubmed
,
Xenbase
Ma,
ER transport signals and trafficking of potassium channels and receptors.
2002,
Pubmed
McCrossan,
The MinK-related peptides.
2004,
Pubmed
,
Xenbase
Minor,
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
2000,
Pubmed
,
Xenbase
Nadal,
The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels.
2003,
Pubmed
,
Xenbase
Niwa,
Molecular determinants of cardiac transient outward potassium current (I(to)) expression and regulation.
2010,
Pubmed
Patel,
Elucidating KChIP effects on Kv4.3 inactivation and recovery kinetics with a minimal KChIP2 isoform.
2002,
Pubmed
,
Xenbase
Patel,
Regulation of Kv4.3 voltage-dependent gating kinetics by KChIP2 isoforms.
2004,
Pubmed
,
Xenbase
Patel,
Heterogeneous expression of KChIP2 isoforms in the ferret heart.
2002,
Pubmed
,
Xenbase
Patel,
Transient outward potassium current, 'Ito', phenotypes in the mammalian left ventricle: underlying molecular, cellular and biophysical mechanisms.
2005,
Pubmed
Pérez-García,
Kvbeta1.2 subunit coexpression in HEK293 cells confers O2 sensitivity to kv4.2 but not to Shaker channels.
1999,
Pubmed
Pioletti,
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
2006,
Pubmed
,
Xenbase
Pourrier,
The Kv4.2 N-terminal restores fast inactivation and confers KChlP2 modulatory effects on N-terminal-deleted Kv1.4 channels.
2004,
Pubmed
Radicke,
Expression and function of dipeptidyl-aminopeptidase-like protein 6 as a putative beta-subunit of human cardiac transient outward current encoded by Kv4.3.
2005,
Pubmed
Rasmusson,
Inactivation of voltage-gated cardiac K+ channels.
1998,
Pubmed
Rasmusson,
C-type inactivation controls recovery in a fast inactivating cardiac K+ channel (Kv1.4) expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Sah,
Regulation of cardiac excitation-contraction coupling by action potential repolarization: role of the transient outward potassium current (I(to)).
2003,
Pubmed
Schrader,
PKA modulation of Kv4.2-encoded A-type potassium channels requires formation of a supramolecular complex.
2002,
Pubmed
,
Xenbase
Shahidullah,
The link between ion permeation and inactivation gating of Kv4 potassium channels.
2003,
Pubmed
,
Xenbase
Shibata,
A fundamental role for KChIPs in determining the molecular properties and trafficking of Kv4.2 potassium channels.
2003,
Pubmed
Skerritt,
Non-native R1 substitution in the s4 domain uniquely alters Kv4.3 channel gating.
2008,
Pubmed
,
Xenbase
Skerritt,
Contribution of electrostatic and structural properties of Kv4.3 S4 arginine residues to the regulation of channel gating.
2009,
Pubmed
,
Xenbase
Skerritt,
Role of S4 positively charged residues in the regulation of Kv4.3 inactivation and recovery.
2007,
Pubmed
,
Xenbase
Skerritt,
KV4.3 expression and gating: S2 and S3 acidic and S4 innermost basic residues.
2009,
Pubmed
Thompson,
IA in play.
2007,
Pubmed
Thomsen,
Transcriptional and electrophysiological consequences of KChIP2-mediated regulation of CaV1.2.
2009,
Pubmed
Thomsen,
Accessory subunit KChIP2 modulates the cardiac L-type calcium current.
2009,
Pubmed
Tombola,
How does voltage open an ion channel?
2006,
Pubmed
Vacher,
Localization and targeting of voltage-dependent ion channels in mammalian central neurons.
2008,
Pubmed
Wang,
Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits.
2007,
Pubmed
,
Xenbase
Wang,
Voltage-dependent gating rearrangements in the intracellular T1-T1 interface of a K+ channel.
2006,
Pubmed
,
Xenbase
Yang,
Kvbeta subunits increase expression of Kv4.3 channels by interacting with their C termini.
2001,
Pubmed
Yellen,
The moving parts of voltage-gated ion channels.
1998,
Pubmed
Zagotta,
Restoration of inactivation in mutants of Shaker potassium channels by a peptide derived from ShB.
1990,
Pubmed
,
Xenbase
Zhang,
minK-related peptide 1 associates with Kv4.2 and modulates its gating function: potential role as beta subunit of cardiac transient outward channel?
2001,
Pubmed
,
Xenbase
Zhou,
Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.
2001,
Pubmed
,
Xenbase
