XB-ART-4907
Genes Dev
2003 Aug 01;1715:1894-908. doi: 10.1101/gad.1084203.
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Sequence-independent DNA binding and replication initiation by the human origin recognition complex.
Vashee S
,
Cvetic C
,
Lu W
,
Simancek P
,
Kelly TJ
,
Walter JC
.
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We report that a highly purified human origin recognition complex (HsORC) has intrinsic DNA-binding activity, and that this activity is modestly stimulated by ATP. HsORC binds preferentially to synthetic AT-rich polydeoxynucleotides, but does not effectively discriminate between natural DNA fragments that contain known human origins and control fragments. The complex fully restores DNA replication to ORC-depleted Xenopus egg extracts, providing strong evidence for its initiator function. Strikingly, HsORC stimulates initiation from any DNA sequence, and it does not preferentially replicate DNA containing human origin sequences. These data provide a biochemical explanation for the observation that in metazoans, initiation of DNA replication often occurs in a seemingly random pattern, and they have important implications for the nature of human origins of DNA replication.
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References [+] :
Abdurashidova,
Start sites of bidirectional DNA synthesis at the human lamin B2 origin.
2000, Pubmed
Abdurashidova, Start sites of bidirectional DNA synthesis at the human lamin B2 origin. 2000, Pubmed
Aladjem, Participation of the human beta-globin locus control region in initiation of DNA replication. 1995, Pubmed
Altman, The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity. 2001, Pubmed
Antequera, CpG islands as genomic footprints of promoters that are associated with replication origins. 1999, Pubmed
Austin, Drosophila ORC specifically binds to ACE3, an origin of DNA replication control element. 1999, Pubmed
Bell, ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. 1992, Pubmed
Bell, DNA replication in eukaryotic cells. 2002, Pubmed
Blow, Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. 1986, Pubmed , Xenbase
Blow, A role for the nuclear envelope in controlling DNA replication within the cell cycle. 1988, Pubmed , Xenbase
Caddle, Intramolecular DNA triplexes, bent DNA and DNA unwinding elements in the initiation region of an amplified dihydrofolate reductase replicon. 1990, Pubmed
Challberg, Adenovirus DNA replication in vitro. 1979, Pubmed
Chaudhuri, Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus. 2001, Pubmed
Chesnokov, Functional analysis of mutant and wild-type Drosophila origin recognition complex. 2001, Pubmed
Chuang, The fission yeast homologue of Orc4p binds to replication origin DNA via multiple AT-hooks. 1999, Pubmed
Chuang, Purification and characterization of the Schizosaccharomyces pombe origin recognition complex: interaction with origin DNA and Cdc18 protein. 2002, Pubmed
Cimbora, Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region. 2000, Pubmed
Connelly, The promoters for human DNA-PKcs (PRKDC) and MCM4: divergently transcribed genes located at chromosome 8 band q11. 1998, Pubmed
Dhar, Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin. 2001, Pubmed
Dhar, Architecture of the human origin recognition complex. 2001, Pubmed
Dijkwel, Dispersive initiation of replication in the Chinese hamster rhodopsin locus. 2000, Pubmed
Dijkwel, Initiation sites are distributed at frequent intervals in the Chinese hamster dihydrofolate reductase origin of replication but are used with very different efficiencies. 2002, Pubmed
Dijkwel, The Chinese hamster dihydrofolate reductase origin consists of multiple potential nascent-strand start sites. 1995, Pubmed
Edwards, MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts. 2002, Pubmed , Xenbase
Frappier, Overproduction, purification, and characterization of EBNA1, the origin binding protein of Epstein-Barr virus. 1991, Pubmed
Fujita, Cell cycle regulation of DNA replication initiation proteins in mammalian cells. 1999, Pubmed
Gilbert, Making sense of eukaryotic DNA replication origins. 2001, Pubmed
Gilbert, Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure. 1995, Pubmed , Xenbase
Gossen, A Drosophila homolog of the yeast origin recognition complex. 1995, Pubmed
Hua, Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2. 1998, Pubmed , Xenbase
Huth, The solution structure of an HMG-I(Y)-DNA complex defines a new architectural minor groove binding motif. 1997, Pubmed
Hyrien, Plasmid replication in Xenopus eggs and egg extracts: a 2D gel electrophoretic analysis. 1992, Pubmed , Xenbase
Hyrien, Chromosomal replication initiates and terminates at random sequences but at regular intervals in the ribosomal DNA of Xenopus early embryos. 1993, Pubmed , Xenbase
Ikeda, Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription. 1986, Pubmed
Ina, A broad replication origin of Drosophila melanogaster, oriDalpha, consists of AT-rich multiple discrete initiation sites. 2001, Pubmed
Keller, The origin recognition complex marks a replication origin in the human TOP1 gene promoter. 2002, Pubmed
Kelly, Regulation of chromosome replication. 2000, Pubmed
Kim, Multiple orientation-dependent, synergistically interacting, similar domains in the ribosomal DNA replication origin of the fission yeast, Schizosaccharomyces pombe. 1998, Pubmed
Klemm, Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex. 1997, Pubmed
Kong, Site-specific DNA binding of the Schizosaccharomyces pombe origin recognition complex is determined by the Orc4 subunit. 2001, Pubmed
Kreitz, The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells. 2001, Pubmed , Xenbase
Krysan, Autonomous replication in human cells of multimers of specific human and bacterial DNA sequences. 1993, Pubmed
Labib, Is the MCM2-7 complex the eukaryotic DNA replication fork helicase? 2001, Pubmed
Ladenburger, Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication. 2002, Pubmed
Lee, Essential lysine residues in the RNA polymerase domain of the gene 4 primase-helicase of bacteriophage T7. 2001, Pubmed
Lee, The Schizosaccharomyces pombe origin recognition complex interacts with multiple AT-rich regions of the replication origin DNA by means of the AT-hook domains of the spOrc4 protein. 2001, Pubmed
Little, Initiation and termination of DNA replication in human rRNA genes. 1993, Pubmed
Mahbubani, DNA replication initiates at multiple sites on plasmid DNA in Xenopus egg extracts. 1992, Pubmed , Xenbase
Méndez, Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis. 2000, Pubmed , Xenbase
Moon, Identification and reconstitution of the origin recognition complex from Schizosaccharomyces pombe. 1999, Pubmed , Xenbase
Natale, Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals. 2000, Pubmed , Xenbase
Newport, Nuclear reconstitution in vitro: stages of assembly around protein-free DNA. 1987, Pubmed , Xenbase
Okuno, Clustered adenine/thymine stretches are essential for function of a fission yeast replication origin. 1999, Pubmed
Okuno, Stability, chromatin association and functional activity of mammalian pre-replication complex proteins during the cell cycle. 2001, Pubmed , Xenbase
Prasanth, Orc6 involved in DNA replication, chromosome segregation, and cytokinesis. 2002, Pubmed
Romanowski, The Xenopus origin recognition complex is essential for DNA replication and MCM binding to chromatin. 1996, Pubmed , Xenbase
Rowles, Changes in association of the Xenopus origin recognition complex with chromatin on licensing of replication origins. 1999, Pubmed , Xenbase
Rowles, Interaction between the origin recognition complex and the replication licensing system in Xenopus. 1996, Pubmed , Xenbase
Royzman, ORC localization in Drosophila follicle cells and the effects of mutations in dE2F and dDP. 1999, Pubmed
Sasaki, Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster. 1999, Pubmed
Schepers, Human origin recognition complex binds to the region of the latent origin of DNA replication of Epstein-Barr virus. 2001, Pubmed
Simpson, Nucleosome positioning can affect the function of a cis-acting DNA element in vivo. 1990, Pubmed
Sun, Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin. 2002, Pubmed , Xenbase
Takahashi, Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins. 2001, Pubmed
Takisawa, Eukaryotic DNA replication: from pre-replication complex to initiation complex. 2000, Pubmed
Todorovic, Replication origins of mammalian chromosomes: the happy few. 1999, Pubmed
Vashee, Assembly of the human origin recognition complex. 2001, Pubmed
von Hippel, Non-specific DNA binding of genome regulating proteins as a biological control mechanism: I. The lac operon: equilibrium aspects. 1974, Pubmed
Walter, Regulation of replicon size in Xenopus egg extracts. 1997, Pubmed , Xenbase
Walter, Evidence for sequential action of cdc7 and cdk2 protein kinases during initiation of DNA replication in Xenopus egg extracts. 2000, Pubmed , Xenbase
Walter, Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. 2000, Pubmed , Xenbase
Walter, Regulated chromosomal DNA replication in the absence of a nucleus. 1998, Pubmed , Xenbase
Wu, A distinct G1 step required to specify the Chinese hamster DHFR replication origin. 1996, Pubmed , Xenbase