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We have examined the proteins ofXenopus whole oocytes and embryos that are accessible to surface iodination. These cell surface proteins appear to undergo stagespecific modulation during development. The most pronounced changes were observed between oocytes and the 32-cell stage when the number of labelled proteins doubled, and between early and late gastrula when the complexity declined by two thirds. The simplification of the pattern during gastrulation may reflect changes in cell position as endodermal cells move inside leaving ectodermal cells at the external surface. The lectin binding patterns for NP-40 solubilized proteins extracted from oocytes and embryos also changed in a stage-dependent manner. Con A and WGA recognized a complex pattern of glycoproteins containing glucose and mannose residues. In contrast, SAB and RCA binding to galactose residues recognized far fewer glycoproteins. Many of the observed changes occurred during cleavage stages before embryonic gene transcription is initiated and may be due to post-translational modifications.
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979, Pubmed,
Xenbase
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Bloodgood,
A flagellar surface glycoprotein mediating cell-substrate interaction in Chlamydomonas.
1984,
Pubmed
Cook,
Cell surface carbohydrates: molecules in search of a function?
1986,
Pubmed
Currie,
Developmentally regulated plasmalemmal glycoconjugates of the surface and neural ectoderm.
1984,
Pubmed
Darribere,
Cell surface proteins in the early embryogenesis of Pleurodeles waltlii.
1982,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Edelman,
Cell adhesion molecules.
1983,
Pubmed
Gimlich,
Early cellular interactions promote embryonic axis formation in Xenopus laevis.
1984,
Pubmed
,
Xenbase
Gualandris,
Membrane changes in neural target cells studied with fluorescent lectin probes.
1983,
Pubmed
Guthrie,
Patterns of junctional communication in the early amphibian embryo.
,
Pubmed
,
Xenbase
Johnson,
The binding of concanavalin A to dissociated embryonic amphibian cells.
1976,
Pubmed
Kalt,
The relationship between cleavage and blastocoel formation in Xenopus laevis. II. Electron microscopic observations.
1971,
Pubmed
,
Xenbase
Kao,
Lithium-induced respecification of pattern in Xenopus laevis embryos.
,
Pubmed
,
Xenbase
King,
Do Xenopus oocytes have a heat shock response?
1987,
Pubmed
,
Xenbase
Moran,
The inhibitory effects of concanavalin A on the development of the amphibian embryo.
1974,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
O'Dell,
The pattern of concanavalin A-binding sites during the early development of Xenopus laevis.
1974,
Pubmed
,
Xenbase
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Riou,
Cell surface glycoproteins change during gastrulation in Pleurodeles waltlii.
1986,
Pubmed
Ruoslahti,
New perspectives in cell adhesion: RGD and integrins.
1987,
Pubmed
Slack,
Peanut lectin receptors in the early amphibian embryo: regional markers for the study of embryonic induction.
1985,
Pubmed
,
Xenbase
Smith,
Measurement of protein using bicinchoninic acid.
1985,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Wessel,
Sequential expression of germ-layer specific molecules in the sea urchin embryo.
1985,
Pubmed
Yamada,
Cell surface interactions with extracellular materials.
1983,
Pubmed
