Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2010 Feb 03;293:532-45. doi: 10.1038/emboj.2009.379.
Show Gene links
Show Anatomy links
Identification of a novel Bves function: regulation of vesicular transport.
Hager HA
,
Roberts RJ
,
Cross EE
,
Proux-Gillardeaux V
,
Bader DM
.
???displayArticle.abstract??? Blood vessel/epicardial substance (Bves) is a transmembrane protein that influences cell adhesion and motility through unknown mechanisms. We have discovered that Bves directly interacts with VAMP3, a SNARE protein that facilitates vesicular transport and specifically recycles transferrin and beta-1-integrin. Two independent assays document that cells expressing a mutated form of Bves are severely impaired in the recycling of these molecules, a phenotype consistent with disruption of VAMP3 function. Using Morpholino knockdown in Xenopus laevis, we demonstrate that elimination of Bves function specifically inhibits transferrin receptor recycling, and results in gastrulation defects previously reported with impaired integrin-dependent cell movements. Kymographic analysis of Bves-depleted primary and cultured cells reveals severe impairment of cell spreading and adhesion on fibronectin, indicative of disruption of integrin-mediated adhesion. Taken together, these data demonstrate that Bves interacts with VAMP3 and facilitates receptor recycling both in vitro and during early development. Thus, this study establishes a newly identified role for Bves in vesicular transport and reveals a novel, broadly applied mechanism governing SNARE protein function.
Andrée,
Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart.
2000, Pubmed
Andrée,
Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart.
2000,
Pubmed
Andrée,
Mouse Pop1 is required for muscle regeneration in adult skeletal muscle.
2002,
Pubmed
Bajno,
Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation.
2000,
Pubmed
Bhattacharya,
Members of the synaptobrevin/vesicle-associated membrane protein (VAMP) family in Drosophila are functionally interchangeable in vivo for neurotransmitter release and cell viability.
2002,
Pubmed
Borisovska,
v-SNAREs control exocytosis of vesicles from priming to fusion.
2005,
Pubmed
Brand,
The Popeye domain-containing gene family.
2005,
Pubmed
Breton,
Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract.
2000,
Pubmed
Brunger,
Structure and function of SNARE and SNARE-interacting proteins.
2005,
Pubmed
Bryan,
GEFT, a Rho family guanine nucleotide exchange factor, regulates neurite outgrowth and dendritic spine formation.
2004,
Pubmed
Bryan,
The Rho-family guanine nucleotide exchange factor GEFT enhances retinoic acid- and cAMP-induced neurite outgrowth.
2006,
Pubmed
Bryant,
The ins and outs of E-cadherin trafficking.
2004,
Pubmed
Cai,
Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle.
2007,
Pubmed
Caswell,
Integrin trafficking and the control of cell migration.
2006,
Pubmed
Caswell,
Endocytic transport of integrins during cell migration and invasion.
2008,
Pubmed
Davidson,
Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension.
2006,
Pubmed
,
Xenbase
Deák,
Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion.
2006,
Pubmed
DeMali,
Integrin signaling to the actin cytoskeleton.
2003,
Pubmed
Desclozeaux,
Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis.
2008,
Pubmed
DeSimone,
The Xenopus embryo as a model system for studies of cell migration.
2005,
Pubmed
,
Xenbase
Dünnwald,
Detection of transient in vivo interactions between substrate and transporter during protein translocation into the endoplasmic reticulum.
1999,
Pubmed
Etienne-Manneville,
Rho GTPases in cell biology.
2002,
Pubmed
Fackler,
Cell motility through plasma membrane blebbing.
2008,
Pubmed
Feng,
DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients.
2008,
Pubmed
Fields,
v-SNARE cellubrevin is required for basolateral sorting of AP-1B-dependent cargo in polarized epithelial cells.
2007,
Pubmed
Galli,
Tetanus toxin-mediated cleavage of cellubrevin impairs exocytosis of transferrin receptor-containing vesicles in CHO cells.
1994,
Pubmed
Grosshans,
Rabs and their effectors: achieving specificity in membrane traffic.
2006,
Pubmed
Guo,
A Rac/Cdc42-specific exchange factor, GEFT, induces cell proliferation, transformation, and migration.
2003,
Pubmed
Hager,
Bves: ten years after.
2009,
Pubmed
Hirohashi,
Inactivation of the E-cadherin-mediated cell adhesion system in human cancers.
1998,
Pubmed
Holly,
Multiple roles of integrins in cell motility.
2000,
Pubmed
Jahn,
SNAREs--engines for membrane fusion.
2006,
Pubmed
Johnson,
Circus movements and blebbing locomotion in dissociated embryonic cells of an amphibian, Xenopus laevis.
1976,
Pubmed
,
Xenbase
Johnson,
Mesodermal cell adhesion to fibronectin-rich fibrillar extracellular matrix is required for normal Rana pipiens gastrulation.
1993,
Pubmed
Kawaguchi,
Identification of a novel intracellular interaction domain essential for Bves function.
2008,
Pubmed
Keller,
The cellular basis of epiboly: an SEM study of deep-cell rearrangement during gastrulation in Xenopus laevis.
1980,
Pubmed
,
Xenbase
Knight,
Membrane topology of Bves/Pop1A, a cell adhesion molecule that displays dynamic changes in cellular distribution during development.
2003,
Pubmed
Kumano,
Revisions to the Xenopus gastrula fate map: implications for mesoderm induction and patterning.
2002,
Pubmed
,
Xenbase
Lanzetti,
Actin in membrane trafficking.
2007,
Pubmed
Leabu,
Membrane fusion in cells: molecular machinery and mechanisms.
2006,
Pubmed
Lin,
Blood vessel/epicardial substance (bves) expression, essential for embryonic development, is down regulated by Grk/EFGR signalling.
2007,
Pubmed
Luftman,
Silencing of VAMP3 inhibits cell migration and integrin-mediated adhesion.
2009,
Pubmed
Marsden,
Integrin-ECM interactions regulate cadherin-dependent cell adhesion and are required for convergent extension in Xenopus.
2003,
Pubmed
,
Xenbase
Marsden,
Regulation of cell polarity, radial intercalation and epiboly in Xenopus: novel roles for integrin and fibronectin.
2001,
Pubmed
,
Xenbase
McCarthy,
Allen Brain Atlas maps 21,000 genes of the mouse brain.
2006,
Pubmed
McMahon,
Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein.
1993,
Pubmed
Mellman,
Coordinated protein sorting, targeting and distribution in polarized cells.
2008,
Pubmed
Na,
Differential regulation of cell adhesive functions by integrin alpha subunit cytoplasmic tails in vivo.
2003,
Pubmed
,
Xenbase
Osler,
Bves expression during avian embryogenesis.
2004,
Pubmed
Osler,
Bves modulates epithelial integrity through an interaction at the tight junction.
2005,
Pubmed
Osler,
Bves, a member of the Popeye domain-containing gene family.
2006,
Pubmed
,
Xenbase
Pfeffer,
Unsolved mysteries in membrane traffic.
2007,
Pubmed
Polgár,
Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion.
2002,
Pubmed
Proux-Gillardeaux,
Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion.
2005,
Pubmed
Ramos,
Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation.
1996,
Pubmed
,
Xenbase
Ramos,
Integrin-dependent adhesive activity is spatially controlled by inductive signals at gastrulation.
1996,
Pubmed
,
Xenbase
Ren,
Migrating anterior mesoderm cells and intercalating trunk mesoderm cells have distinct responses to Rho and Rac during Xenopus gastrulation.
2006,
Pubmed
,
Xenbase
Ridley,
Rho proteins: linking signaling with membrane trafficking.
2001,
Pubmed
Ripley,
Bves is expressed in the epithelial components of the retina, lens, and cornea.
2004,
Pubmed
Ripley,
Xbves is a regulator of epithelial movement during early Xenopus laevis development.
2006,
Pubmed
,
Xenbase
Schwartz,
Signaling networks linking integrins and rho family GTPases.
2000,
Pubmed
Segev,
Ypt/rab gtpases: regulators of protein trafficking.
2001,
Pubmed
Selchow,
Structure and cytoskeletal organization of migratory mesoderm cells from the Xenopus gastrula.
1997,
Pubmed
,
Xenbase
Shook,
Mechanisms, mechanics and function of epithelial-mesenchymal transitions in early development.
2003,
Pubmed
,
Xenbase
Skalski,
SNARE-mediated trafficking of alpha5beta1 integrin is required for spreading in CHO cells.
2005,
Pubmed
Smith,
Bves directly interacts with GEFT, and controls cell shape and movement through regulation of Rac1/Cdc42 activity.
2008,
Pubmed
Smith,
Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins.
1990,
Pubmed
,
Xenbase
Smith,
Characterization of Bves expression during mouse development using newly generated immunoreagents.
2006,
Pubmed
Symons,
Control of vesicular trafficking by Rho GTPases.
2003,
Pubmed
Tajika,
VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration.
2007,
Pubmed
Tayeb,
Inhibition of SNARE-mediated membrane traffic impairs cell migration.
2005,
Pubmed
Torlopp,
Comparative analysis of mRNA and protein expression of Popdc1 (Bves) during early development in the chick embryo.
2006,
Pubmed
Vasavada,
Developmental expression of Pop1/Bves.
2004,
Pubmed
Wada,
Bves: prototype of a new class of cell adhesion molecules expressed during coronary artery development.
2001,
Pubmed
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase
Winklbauer,
Directional mesoderm cell migration in the Xenopus gastrula.
1991,
Pubmed
,
Xenbase
Yap,
Making and breaking contacts: the cellular biology of cadherin regulation.
2007,
Pubmed
