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XB-ART-2531
Dev Cell 2005 Jan 01;81:53-64. doi: 10.1016/j.devcel.2004.12.003.
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POLO kinase regulates the Drosophila centromere cohesion protein MEI-S332.

Clarke AS , Tang TT , Ooi DL , Orr-Weaver TL .


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Accurate segregation of chromosomes is critical to ensure that each daughter cell receives the full genetic complement. Maintenance of cohesion between sister chromatids, especially at centromeres, is required to segregate chromosomes precisely during mitosis and meiosis. The Drosophila protein MEI-S332, the founding member of a conserved protein family, is essential in meiosis for maintaining cohesion at centromeres until sister chromatids separate at the metaphase II/anaphase II transition. MEI-S332 localizes onto centromeres in prometaphase of mitosis or meiosis I, remaining until sister chromatids segregate. We elucidated a mechanism for controlling release of MEI-S332 from centromeres via phosphorylation by POLO kinase. We demonstrate that POLO antagonizes MEI-S332 cohesive function and that full POLO activity is needed to remove MEI-S332 from centromeres, yet this delocalization is not required for sister chromatid separation. POLO phosphorylates MEI-S332 in vitro, POLO and MEI-S332 bind each other, and mutation of POLO binding sites prevents MEI-S332 dissociation from centromeres.

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Species referenced: Xenopus
Genes referenced: plk1

References :
Lake, A new target for POLO in meiotic centromere cohesion. 2004, Pubmed