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Nucleotide sequence encoding the 5' end of Xenopus laevis 18S rRNA.
Salim M
,
Maden BE
.
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We have sequenced a region of cloned Xenopus laevis ribosomal DNA encompassing the last 24 nucleotides of the external transcribed spacer and the first 275 nucleotides of the 18S gene. The start of the 18S gene was identified by correlating the results obtained from RNA hybridization and fingerprinting with the DNA sequence. This 5' region of 18S rRNA contains five 2'-O-methyl groups and at least six pseudouridine residues. Several of these modified nucleotides are clustered into a relatively short region from nucleotides 99-124. Nucleotides 227-250 constitute a distinctive sequence of 24 consecutive G and C residues. Comparison with the first 160 nucleotides of a yeast 18S gene (25) reveals three blocks of high sequence homology separated by two short tracts where homology is low or absent. The external transcribed spacer sequences diverge widely from within a few nucleotides of the start of the 18S gene.
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979, Pubmed,
Xenbase
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Botchan,
Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA.
1977,
Pubmed
,
Xenbase
Brand,
Fine structure of ribosomal RNA. II. Distribution of methylated sequences within Xenopus laevis rRNA.
1979,
Pubmed
,
Xenbase
Brosius,
Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
1978,
Pubmed
Carbon,
The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methods.
1978,
Pubmed
Cox,
Distribution of sequences common to the 25--28S-ribonucleic acid genes of Xenopus laevis and Neurospora crassa.
1980,
Pubmed
,
Xenbase
Eladari,
Sequence determination of 5'-terminal and 3'-terminal T1 oligonucleotides of 18-S ribosomal RNA of a mouse cell line (L 5178 Y).
1975,
Pubmed
Ford,
The nucleotide sequences of 5.8-S ribosomal RNA from Xenopus laevis and Xenopus borealis.
1978,
Pubmed
,
Xenbase
Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed
Hughes,
The pseudouridine contents of the ribosomal ribonucleic acids of three vertebrate species. Numerical correspondence between pseudouridine residues and 2'-O-methyl groups is not always conserved.
1978,
Pubmed
,
Xenbase
Khan,
Extensive homologies between the methylated nucleotide sequences in several vertebrate ribosomal ribonucleic acids.
1978,
Pubmed
,
Xenbase
Khan,
Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs.
1977,
Pubmed
,
Xenbase
Loening,
Molecular weights of ribosomal RNA in relation to evolution.
1968,
Pubmed
Maden,
The methylated nucleotide sequences in HELA cell ribosomal RNA and its precursors.
1974,
Pubmed
Maden,
Standard and non standard products in combined T(1) plus pancreatic RNAase fingerprints of HeLa cell rRNA and its precursors.
1972,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Moss,
More ribosomal spacer sequences from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Nazar,
Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid.
1975,
Pubmed
Petermann,
The subunits and structural ribonucleic acids of Jensen sarcoma ribosomes.
1966,
Pubmed
Sakuma,
Conservation of the 5'-terminal nucleotide sequences of ribosomal 18-S RNA in eukaryotes. Differential evolution of large and small ribosomal RNA.
1976,
Pubmed
,
Xenbase
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Sutcliffe,
pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
1978,
Pubmed
Trendelenburg,
Transcription of cloned Xenopus ribosomal genes visualised after injection into oocyte nuclei.
1978,
Pubmed
,
Xenbase
