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Electron microscopic study of crystals of the Xenopus laevis transcription factor IIIA-5S ribosomal RNA complex.
Brown RS
,
Ferguson C
,
Kingswell A
,
Winkler FK
,
Leonard KR
.
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A novel method has been developed to grow crystals of the Xenopus laevis transcription factor IIIA-5S RNA complex directly on grids for examination by electron microscopy. Microcrystals were examined in negative stain and in thin sections to reveal a hexagonal lattice with unit-cell dimensions a = b = 87.1 +/- 4.4 A and c = 143.8 +/- 12.7 A. Optical diffraction patterns from micrographs were obtained about the major crystal axes extending to about 40-A resolution. A packing scheme is proposed for which there are three or six transcription factor IIIA-5S RNA complexes in the unit cell related by 3(1) symmetry along the long cell axis. This would require that the 5S RNA molecules are arranged end-to-end, with the terminal loops of adjacent molecules overlapping.
Abdel-Meguid,
Crystallization of a ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA and its complex with protein L25.
1983, Pubmed
Abdel-Meguid,
Crystallization of a ribonuclease-resistant fragment of Escherichia coli 5 S ribosomal RNA and its complex with protein L25.
1983,
Pubmed
Barrett,
Specific interaction of proteins with 5 S RNA and tRNA in the 42 S storage particle of Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Berriman,
Methods for specimen thickness determination in electron microscopy. II. Changes in thickness with dose.
1986,
Pubmed
Bieker,
Physical properties and DNA-binding stoichiometry of a 5 S gene-specific transcription factor.
1984,
Pubmed
,
Xenbase
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Brown,
Fingers and helices.
,
Pubmed
,
Xenbase
Chothia,
Principles that determine the structure of proteins.
1984,
Pubmed
Chowdhury,
A multigene family encoding several "finger" structures is present and differentially active in mammalian genomes.
1987,
Pubmed
,
Xenbase
Christiansen,
Xenopus transcription factor IIIA binds primarily at junctions between double helical stems and internal loops in oocyte 5S RNA.
1987,
Pubmed
,
Xenbase
Denis,
Thesaurisomes, a novel kind of nucleoprotein particle.
1983,
Pubmed
,
Xenbase
Denis,
Biochemical research on oogenesis: oocytes of Xenopus laevis synthesize but do not accumulate 5S RNA of somatic type.
1977,
Pubmed
,
Xenbase
Denis,
Biochemical research on oogenesis. The storage particles of the teleost fish Tinca tinca.
1980,
Pubmed
,
Xenbase
Diakun,
EXAFS study of the zinc-binding sites in the protein transcription factor IIIA.
,
Pubmed
,
Xenbase
Fairall,
Mapping of the sites of protection on a 5 S RNA gene by the Xenopus transcription factor IIIA. A model for the interaction.
1986,
Pubmed
,
Xenbase
Ford,
Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Frankel,
Metal-dependent folding of a single zinc finger from transcription factor IIIA.
1987,
Pubmed
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase
Jésior,
The grid sectioning technique: a study of catalase platelets.
1982,
Pubmed
Leonard,
Three-dimensional structure of ubiquinol:cytochrome c reductase from Neurospora mitochondria determined by electron microscopy of membrane crystals.
1981,
Pubmed
Mattaj,
Nuclear exclusion of transcription factor IIIA and the 42s particle transfer RNA-binding protein in Xenopus oocytes: a possible mechanism for gene control?
1983,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Morikawa,
Electron microscopic study of 5S rRNA crystals from Thermus thermophilus HB8.
1986,
Pubmed
Morikawa,
Crystallization and preliminary X-ray diffraction study of 5 S rRNA from Thermus thermophilus HB8.
1982,
Pubmed
Morikawa,
Single crystals of 5S rRNA from Thermus thermophilus HB8 and preliminary X-ray diffraction data.
1982,
Pubmed
Müller,
Comparison of the structure of ribosomal 5S RNA from E. coli and from rat liver using X-ray scattering and dynamic light scattering.
1986,
Pubmed
Muriel,
Molecular analysis of ouabain-resistant mutants of the mouse lymphoma cell line L5178Y.
1987,
Pubmed
Pays,
DNA-binding fingers encoded by a trypanosome retroposon.
1987,
Pubmed
,
Xenbase
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase
Ruiz i Altaba,
Xfin: an embryonic gene encoding a multifingered protein in Xenopus.
1987,
Pubmed
,
Xenbase
Schuh,
A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.
1986,
Pubmed
,
Xenbase
Taylor,
The developmental expression of the gene for TFIIIA in Xenopus laevis.
1986,
Pubmed
,
Xenbase
Tso,
Structure of the gene for Xenopus transcription factor TFIIIA.
1986,
Pubmed
,
Xenbase
Vincent,
Drosophila maternal and embryo mRNAs transcribed from a single transcription unit use alternate combinations of exons.
1984,
Pubmed
Vincent,
TFIIIA and homologous genes. The 'finger' proteins.
1986,
Pubmed
,
Xenbase
Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase
Westhof,
Crystallographic refinement of yeast aspartic acid transfer RNA.
1985,
Pubmed
