Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-5755
Nat Cell Biol 2003 Mar 01;53:205-10. doi: 10.1038/ncb939.
Show Gene links Show Anatomy links

Reversible disassembly of somatic nucleoli by the germ cell proteins FRGY2a and FRGY2b.

Gonda K , Fowler J , Katoku-Kikyo N , Haroldson J , Wudel J , Kikyo N .


???displayArticle.abstract???
Egg cytoplasm has the capability to reprogramme differentiated somatic nuclei, as shown by nuclear transplantation in animal cloning. The nucleoli of donor nuclei are rapidly disassembled on injection into interphase eggs and are correctly reassembled when donor transcription initiates in the early embryos of frogs and mammals, recapitulating the physiological nucleolar dynamics of early embryogenesis. This is one of the most remarkable structural reorganizations of somatic nuclei in nuclear cloning. Despite the long history of nuclear cloning, almost nothing is known about the molecular mechanism of nucleolar disassembly in egg cytoplasm. Here we show that the Xenopus germ cell proteins FRGY2a and FRGY2b reversibly disassemble somatic nucleoli in egg cytoplasm, independently of continuing ribosomal RNA transcription. The carboxy-terminal domain of FRGY2a, which localizes to the nucleoli, is sufficient for nucleolar disassembly in transfected cells. Our results show that a single protein fragment can trigger reversible disassembly of the complex nucleolar structure.

???displayArticle.pubmedLink??? 12589397
???displayArticle.link??? Nat Cell Biol



References :
Misteli, A nucleolar disappearing act in somatic cloning. 2003, Pubmed, Xenbase