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A cap binding protein that may mediate nuclear export of RNA polymerase II-transcribed RNAs.
Izaurralde E
,
Stepinski J
,
Darzynkiewicz E
,
Mattaj IW
.
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It has previously been shown that efficient export of U1 snRNA or of microinjected, in vitro synthesized, RNA transcripts from the nucleus of Xenopus oocytes is facilitated by their monomethyl guanosine cap structures. Nuclear exit of these transcripts could be competitively inhibited by microinjection of an excess of a cap analog, the dinucleotide m7GpppG (Hamm, J., and I. W. Mattaj. 1990. Cell. 63:109-118). We have now analyzed the ability of several other related cap analogs to inhibit the export of U1 snRNA from the nucleus. The results define the recognition specificity of a factor(s) involved in RNA transport, and indicate that the cap binding activity (CBA) involved in RNA export is different from cap binding proteins (CBPs) involved in the initiation of translation. A CBP, whose specificity for different analogs correlates with the ability of the analogs to inhibit U1 snRNA export, is identified in nuclear extracts prepared from HeLa cells. We propose that this protein may have a role in the export of capped RNAs from the nucleus.
Carberry,
A spectroscopic study of the binding of m7GTP and m7GpppG to human protein synthesis initiation factor 4E.
1989, Pubmed
Carberry,
A spectroscopic study of the binding of m7GTP and m7GpppG to human protein synthesis initiation factor 4E.
1989,
Pubmed
Carberry,
A spectroscopic study of the binding of N-7-substituted cap analogues to human protein synthesis initiation factor 4E.
1990,
Pubmed
Chang,
Regulation by HIV Rev depends upon recognition of splice sites.
1989,
Pubmed
Chang,
Messenger RNA transport and HIV rev regulation.
1990,
Pubmed
Darzynkiewicz,
Inhibition of eukaryotic translation by nucleoside 5'-monophosphate analogues of mRNA 5'-cap: changes in N7 substituent affect analogue activity.
1989,
Pubmed
Darzynkiewicz,
Beta-globin mRNAs capped with m7G, m2.7(2)G or m2.2.7(3)G differ in intrinsic translation efficiency.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Dreyfuss,
Structure and function of nuclear and cytoplasmic ribonucleoprotein particles.
1986,
Pubmed
Eckner,
Mature mRNA 3' end formation stimulates RNA export from the nucleus.
1991,
Pubmed
Edery,
Cap-dependent RNA splicing in a HeLa nuclear extract.
1985,
Pubmed
Guddat,
Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes.
1990,
Pubmed
,
Xenbase
Hamm,
Multiple domains of U1 snRNA, including U1 specific protein binding sites, are required for splicing.
1990,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase
Inoue,
Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei.
1989,
Pubmed
,
Xenbase
Konarska,
Recognition of cap structure in splicing in vitro of mRNA precursors.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Legrain,
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
1989,
Pubmed
Leibold,
Cytoplasmic protein binds in vitro to a highly conserved sequence in the 5' untranslated region of ferritin heavy- and light-subunit mRNAs.
1988,
Pubmed
Maquat,
Nuclear mRNA export.
1991,
Pubmed
Martin,
Nuclear transport of influenza virus ribonucleoproteins: the viral matrix protein (M1) promotes export and inhibits import.
1991,
Pubmed
Mehlin,
Transport of Balbiani ring granules through nuclear pores in Chironomus tentans.
1991,
Pubmed
Ohno,
A nuclear cap binding protein from HeLa cells.
1990,
Pubmed
Patzelt,
CAP binding proteins associated with the nucleus.
1983,
Pubmed
Patzelt,
Assembly of pre-mRNA splicing complex is cap dependent.
1987,
Pubmed
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed
Rhoads,
Cap recognition and the entry of mRNA into the protein synthesis initiation cycle.
1988,
Pubmed
Rozen,
Identification of nuclear cap specific proteins in HeLa cells.
1987,
Pubmed
Scherly,
Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.
1989,
Pubmed
,
Xenbase
Sonenberg,
Cap-binding proteins of eukaryotic messenger RNA: functions in initiation and control of translation.
1988,
Pubmed
Sonenberg,
ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.
1981,
Pubmed
Sonenberg,
A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.
1978,
Pubmed
