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Phorbol 12-myristate 13-acetate down-regulates Na,K-ATPase independent of its protein kinase C site: decrease in basolateral cell surface area.
Beron J
,
Forster I
,
Beguin P
,
Geering K
,
Verrey F
.
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The effect of protein kinase C (PKC) stimulation on the pump current (Ip) generated by the Na,K-ATPase was measured in A6 epithelia apically permeabilized with amphotericin B. Phorbol 12-myristate 13-acetate (PMA) produced a decrease in Ip carried by sodium pumps containing the endogenous Xenopus laevis or transfected Bufo marinus alpha 1 subunits (approximately 30% reduction within 25 min, maximum after 40 min) independent of the PKC phosphorylation site (T15A/S16A). In addition to this major effect of PMA, which was independent of the intracellular sodium concentration and was prevented by the PKC inhibitor bisindolylmaleimide GF 109203X (BIM), another BIM-resistant, PKC site-independent decrease was observed when the Ip was measured at low sodium concentrations (total reduction approximately 50% at 5 mM sodium). Using ouabain binding and cell surface biotinylation, stimulation of PKC was shown to reduce surface Na,K-ATPase by 14 to 20% within 25 min. The same treatment stimulated fluid phase endocytosis sevenfold and decreased by 16.5% the basolateral cell surface area measured by transepithelial capacitance measurements. In conclusion, PKC stimulation produces a decrease in sodium pump function which can be attributed, to a large extent, to a withdrawal of sodium pumps from the basolateral cell surface independent of their PKC site. This reduction of the number of sodium pumps is parallel to a decrease in basolateral membrane area.
Beguin,
Phosphorylation of the Na,K-ATPase alpha-subunit by protein kinase A and C in vitro and in intact cells. Identification of a novel motif for PKC-mediated phosphorylation.
1994, Pubmed,
Xenbase
Beguin,
Phosphorylation of the Na,K-ATPase alpha-subunit by protein kinase A and C in vitro and in intact cells. Identification of a novel motif for PKC-mediated phosphorylation.
1994,
Pubmed
,
Xenbase
Beron,
Aldosterone modulates sodium kinetics of Na,K-ATPase containing an alpha 1 subunit in A6 kidney cell epithelia.
1995,
Pubmed
Beron,
Aldosterone induces early activation and late accumulation of Na-K-ATPase at surface of A6 cells.
1994,
Pubmed
,
Xenbase
Bertorello,
Phosphorylation of the catalytic subunit of Na+,K(+)-ATPase inhibits the activity of the enzyme.
1991,
Pubmed
Bertorello,
Na+-K+-ATPase is an effector protein for protein kinase C in renal proximal tubule cells.
1989,
Pubmed
Chibalin,
Phosphorylation of Na,K-ATPase alpha-subunits in microsomes and in homogenates of Xenopus oocytes resulting from the stimulation of protein kinase A and protein kinase C.
1992,
Pubmed
,
Xenbase
Eaton,
Renal sodium channels: regulation and single channel properties.
1995,
Pubmed
Ewart,
Hormonal regulation of the Na(+)-K(+)-ATPase: mechanisms underlying rapid and sustained changes in pump activity.
1995,
Pubmed
Féraille,
Protein kinase C-dependent stimulation of Na(+)-K(+)-ATP epsilon in rat proximal convoluted tubules.
1995,
Pubmed
Feschenko,
Structural basis for species-specific differences in the phosphorylation of Na,K-ATPase by protein kinase C.
1995,
Pubmed
Frindt,
Feedback regulation of Na channels in rat CCT. IV. Mediation by activation of protein kinase C.
1996,
Pubmed
Gottardi,
Biotinylation and assessment of membrane polarity: caveats and methodological concerns.
1995,
Pubmed
Hays,
Effects of protein kinase C activation on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule.
1987,
Pubmed
Jaisser,
Primary sequence and functional expression of a novel ouabain-resistant Na,K-ATPase. The beta subunit modulates potassium activation of the Na,K-pump.
1992,
Pubmed
,
Xenbase
Ling,
Effects of luminal Na+ on single Na+ channels in A6 cells, a regulatory role for protein kinase C.
1989,
Pubmed
Ling,
Inhibition of apical Na+ channels in rabbit cortical collecting tubules by basolateral prostaglandin E2 is modulated by protein kinase C.
1992,
Pubmed
Lowndes,
Kinetics of phosphorylation of Na+/K(+)-ATPase by protein kinase C.
1990,
Pubmed
Martiny-Baron,
Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976.
1993,
Pubmed
Middleton,
Heterogeneity of protein kinase C-mediated rapid regulation of Na/K-ATPase in kidney epithelial cells.
1993,
Pubmed
Satoh,
Different mechanisms of renal Na-K-ATPase regulation by protein kinases in proximal and distal nephron.
1993,
Pubmed
Shahedi,
Protein kinase C activation causes inhibition of Na/K-ATPase activity in Madin-Darby canine kidney epithelial (MDCK) cells.
1992,
Pubmed
Takeda,
Inhibition of amiloride-sensitive apical Na+ conductance by acetylcholine in rabbit cortical collecting duct perfused in vitro.
1994,
Pubmed
Toullec,
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
1991,
Pubmed
Vasilets,
Activation of protein kinase C by phorbol ester induces downregulation of the Na+/K(+)-ATPase in oocytes of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Verrey,
Polarized membrane movements in A6 kidney cells are regulated by aldosterone and vasopressin/vasotocin.
1993,
Pubmed
,
Xenbase
Verrey,
Corticosteroid regulation of renal Na,K-ATPase.
1996,
Pubmed
Verrey,
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
1994,
Pubmed
,
Xenbase
Wilborn,
Differential expression of PKC isoforms in fresh and cultured rabbit CCD.
1996,
Pubmed
Wilkinson,
Protein kinase C: is its pivotal role in cellular activation over-stated?
1994,
Pubmed
Yanase,
Activators of protein kinase C inhibit sodium transport in A6 epithelia.
1986,
Pubmed
,
Xenbase
