Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos.
Platonova O
,
Akey IV
,
Head JF
,
Akey CW
.
???displayArticle.abstract???
Human Npm2 is an ortholog of Xenopus nucleoplasmin (Np), a chaperone that binds histones. We have determined the crystal structure of a truncated Npm2-core at 1.9 Å resolution and show that the N-terminal domains of Npm2 and Np form similar pentamers. This allowed us to model an Npm2 decamer which may be formed by hydrogen bonds between quasi-conserved residues in the interface between two pentamers. Interestingly, the Npm2 pentamer lacks a prototypical A1-acidic tract in each of its subunits. This feature may be responsible for the inability of Npm2-core to bind histones. However, Npm2 with a large acidic tract in its C-terminal tail (Npm2-A2) is able to bind histones and form large complexes. Fluorescence resonance energy transfer experiments and biochemical analysis of loop mutations support the premise that nucleoplasmins form decamers when they bind H2A-H2B dimers and H3-H4 tetramers simultaneously. In the absence of histone tetramers, these chaperones bind H2A-H2B dimers with a single pentamer forming the central hub. When taken together, our data provide insights into the mechanism of histone binding by nucleoplasmins.
Adams,
PHENIX: building new software for automated crystallographic structure determination.
2002, Pubmed
Adams,
PHENIX: building new software for automated crystallographic structure determination.
2002,
Pubmed
Akey,
Histone chaperones and nucleosome assembly.
2003,
Pubmed
Bañuelos,
Phosphorylation of both nucleoplasmin domains is required for activation of its chromatin decondensation activity.
2007,
Pubmed
,
Xenbase
Bañuelos,
Activation mechanism of the nuclear chaperone nucleoplasmin: role of the core domain.
2003,
Pubmed
,
Xenbase
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Burns,
Roles of NPM2 in chromatin and nucleolar organization in oocytes and embryos.
2003,
Pubmed
,
Xenbase
Cotten,
Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs.
1986,
Pubmed
,
Xenbase
De La Fuente,
Major chromatin remodeling in the germinal vesicle (GV) of mammalian oocytes is dispensable for global transcriptional silencing but required for centromeric heterochromatin function.
2004,
Pubmed
Dutta,
The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.
2001,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
Goddard,
Software extensions to UCSF chimera for interactive visualization of large molecular assemblies.
2005,
Pubmed
Hierro,
Electrostatic interactions at the C-terminal domain of nucleoplasmin modulate its chromatin decondensation activity.
2002,
Pubmed
,
Xenbase
Hierro,
Structural and functional properties of Escherichia coli-derived nucleoplasmin. A comparative study of recombinant and natural proteins.
2001,
Pubmed
,
Xenbase
Ito,
ATP-facilitated chromatin assembly with a nucleoplasmin-like protein from Drosophila melanogaster.
1996,
Pubmed
,
Xenbase
Kissinger,
Rapid automated molecular replacement by evolutionary search.
1999,
Pubmed
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Kleinschmidt,
Nucleosome assembly in vitro: separate histone transfer and synergistic interaction of native histone complexes purified from nuclei of Xenopus laevis oocytes.
1990,
Pubmed
,
Xenbase
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Lee,
Crystal structure of human nucleophosmin-core reveals plasticity of the pentamer-pentamer interface.
2007,
Pubmed
Leno,
Hyperphosphorylation of nucleoplasmin facilitates Xenopus sperm decondensation at fertilization.
1996,
Pubmed
,
Xenbase
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Namboodiri,
The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding.
2003,
Pubmed
Namboodiri,
The structure and function of Xenopus NO38-core, a histone chaperone in the nucleolus.
2004,
Pubmed
,
Xenbase
Okuwaki,
Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone.
2001,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Philpott,
Nuclear chaperones.
2000,
Pubmed
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Ramos,
Nucleoplasmin-mediated unfolding of chromatin involves the displacement of linker-associated chromatin proteins.
2005,
Pubmed
,
Xenbase
Ramos,
Nucleoplasmin binds histone H2A-H2B dimers through its distal face.
2010,
Pubmed
,
Xenbase
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Salvany,
Mutation of the small acidic tract A1 drastically reduces nucleoplasmin activity.
2004,
Pubmed
,
Xenbase
Studier,
Protein production by auto-induction in high density shaking cultures.
2005,
Pubmed
Taneva,
Activation of nucleoplasmin, an oligomeric histone chaperone, challenges its stability.
2008,
Pubmed
Taneva,
A mechanism for histone chaperoning activity of nucleoplasmin: thermodynamic and structural models.
2009,
Pubmed
,
Xenbase
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Ye,
Defective S phase chromatin assembly causes DNA damage, activation of the S phase checkpoint, and S phase arrest.
2003,
Pubmed
Zucker,
The histone H3/H4.N1 complex supplemented with histone H2A-H2B dimers and DNA topoisomerase I forms nucleosomes on circular DNA under physiological conditions.
1990,
Pubmed
,
Xenbase
